Experiment: KB with Lawsone 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1269 and Psyr_1270 are separated by 5 nucleotides Psyr_1270 and Psyr_1271 are separated by 121 nucleotides Psyr_1271 and Psyr_1272 are separated by 61 nucleotides Psyr_1272 and Psyr_1273 are separated by 150 nucleotides
Psyr_1269: Psyr_1269 - phosphoribosylformylglycinamidine synthase, at 1,433,337 to 1,437,233
_1269
Psyr_1270: Psyr_1270 - conserved hypothetical protein, at 1,437,239 to 1,437,550
_1270
Psyr_1271: Psyr_1271 - ErfK/YbiS/YcfS/YnhG, at 1,437,672 to 1,438,235
_1271
Psyr_1272: Psyr_1272 - NUDIX hydrolase, at 1,438,297 to 1,438,848
_1272
Psyr_1273: Psyr_1273 - NUDIX hydrolase, at 1,438,999 to 1,439,595
_1273
Position (kb)
1437
1438
1439 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1437.231 kb on + strand at 1437.231 kb on + strand at 1437.232 kb on - strand at 1437.232 kb on - strand at 1437.242 kb on + strand at 1437.242 kb on + strand at 1437.242 kb on + strand at 1437.243 kb on - strand at 1437.243 kb on - strand at 1437.243 kb on - strand at 1437.330 kb on - strand, within Psyr_1270 at 1437.398 kb on - strand, within Psyr_1270 at 1437.528 kb on + strand at 1437.528 kb on + strand at 1437.529 kb on - strand at 1437.529 kb on - strand at 1437.529 kb on - strand at 1437.529 kb on - strand at 1437.717 kb on + strand at 1437.717 kb on + strand at 1437.718 kb on - strand at 1437.782 kb on - strand, within Psyr_1271 at 1437.782 kb on - strand, within Psyr_1271 at 1437.910 kb on + strand, within Psyr_1271 at 1437.910 kb on + strand, within Psyr_1271 at 1437.910 kb on + strand, within Psyr_1271 at 1437.910 kb on + strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.911 kb on - strand, within Psyr_1271 at 1437.943 kb on + strand, within Psyr_1271 at 1437.943 kb on + strand, within Psyr_1271 at 1437.944 kb on - strand, within Psyr_1271 at 1438.253 kb on + strand at 1438.253 kb on + strand at 1438.254 kb on - strand at 1438.254 kb on - strand at 1438.254 kb on - strand at 1438.255 kb on - strand at 1438.338 kb on + strand at 1438.338 kb on + strand at 1438.379 kb on + strand, within Psyr_1272 at 1438.379 kb on + strand, within Psyr_1272 at 1438.379 kb on + strand, within Psyr_1272 at 1438.379 kb on + strand, within Psyr_1272 at 1438.379 kb on + strand, within Psyr_1272 at 1438.379 kb on + strand, within Psyr_1272 at 1438.380 kb on - strand, within Psyr_1272 at 1438.380 kb on - strand, within Psyr_1272 at 1438.380 kb on - strand, within Psyr_1272 at 1438.380 kb on - strand, within Psyr_1272 at 1438.380 kb on - strand, within Psyr_1272 at 1438.384 kb on + strand, within Psyr_1272 at 1438.384 kb on + strand, within Psyr_1272 at 1438.385 kb on - strand, within Psyr_1272 at 1438.385 kb on - strand, within Psyr_1272 at 1438.385 kb on - strand, within Psyr_1272 at 1438.385 kb on - strand, within Psyr_1272 at 1438.505 kb on + strand, within Psyr_1272 at 1438.505 kb on + strand, within Psyr_1272 at 1438.506 kb on - strand, within Psyr_1272 at 1438.506 kb on - strand, within Psyr_1272 at 1438.527 kb on + strand, within Psyr_1272 at 1438.550 kb on + strand, within Psyr_1272 at 1438.550 kb on + strand, within Psyr_1272 at 1438.550 kb on + strand, within Psyr_1272 at 1438.551 kb on - strand, within Psyr_1272 at 1438.551 kb on - strand, within Psyr_1272 at 1438.749 kb on - strand, within Psyr_1272 at 1438.779 kb on - strand, within Psyr_1272 at 1438.779 kb on - strand, within Psyr_1272 at 1438.779 kb on - strand, within Psyr_1272 at 1438.779 kb on - strand, within Psyr_1272 at 1439.012 kb on + strand at 1439.012 kb on + strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand at 1439.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lawsone 10 mM remove 1,437,231 + +0.3 1,437,231 + +0.8 1,437,232 - +1.9 1,437,232 - +1.6 1,437,242 + +1.2 1,437,242 + -1.9 1,437,242 + +1.2 1,437,243 - +1.4 1,437,243 - +1.0 1,437,243 - +0.3 1,437,330 - Psyr_1270 0.29 +1.5 1,437,398 - Psyr_1270 0.51 -0.5 1,437,528 + -0.8 1,437,528 + -0.2 1,437,529 - -0.4 1,437,529 - +0.1 1,437,529 - -0.5 1,437,529 - -1.8 1,437,717 + +0.3 1,437,717 + -0.3 1,437,718 - +0.3 1,437,782 - Psyr_1271 0.20 -0.3 1,437,782 - Psyr_1271 0.20 -1.0 1,437,910 + Psyr_1271 0.42 -0.8 1,437,910 + Psyr_1271 0.42 -0.8 1,437,910 + Psyr_1271 0.42 -0.3 1,437,910 + Psyr_1271 0.42 -1.1 1,437,911 - Psyr_1271 0.42 +0.5 1,437,911 - Psyr_1271 0.42 -0.2 1,437,911 - Psyr_1271 0.42 +0.9 1,437,911 - Psyr_1271 0.42 +0.8 1,437,911 - Psyr_1271 0.42 +0.7 1,437,911 - Psyr_1271 0.42 +0.6 1,437,911 - Psyr_1271 0.42 -0.7 1,437,943 + Psyr_1271 0.48 +1.3 1,437,943 + Psyr_1271 0.48 +0.3 1,437,944 - Psyr_1271 0.48 -0.4 1,438,253 + +0.0 1,438,253 + -1.7 1,438,254 - -0.2 1,438,254 - -0.7 1,438,254 - +0.4 1,438,255 - -1.4 1,438,338 + -1.0 1,438,338 + -1.7 1,438,379 + Psyr_1272 0.15 +0.1 1,438,379 + Psyr_1272 0.15 -0.1 1,438,379 + Psyr_1272 0.15 +1.6 1,438,379 + Psyr_1272 0.15 -2.6 1,438,379 + Psyr_1272 0.15 +2.1 1,438,379 + Psyr_1272 0.15 +0.8 1,438,380 - Psyr_1272 0.15 +1.4 1,438,380 - Psyr_1272 0.15 -1.4 1,438,380 - Psyr_1272 0.15 +0.1 1,438,380 - Psyr_1272 0.15 +0.9 1,438,380 - Psyr_1272 0.15 -0.2 1,438,384 + Psyr_1272 0.16 +1.6 1,438,384 + Psyr_1272 0.16 -1.6 1,438,385 - Psyr_1272 0.16 +0.7 1,438,385 - Psyr_1272 0.16 -1.3 1,438,385 - Psyr_1272 0.16 +0.1 1,438,385 - Psyr_1272 0.16 -0.9 1,438,505 + Psyr_1272 0.38 +1.1 1,438,505 + Psyr_1272 0.38 -2.3 1,438,506 - Psyr_1272 0.38 -0.7 1,438,506 - Psyr_1272 0.38 -2.0 1,438,527 + Psyr_1272 0.42 -0.2 1,438,550 + Psyr_1272 0.46 -0.3 1,438,550 + Psyr_1272 0.46 -0.3 1,438,550 + Psyr_1272 0.46 +1.7 1,438,551 - Psyr_1272 0.46 -0.4 1,438,551 - Psyr_1272 0.46 -0.2 1,438,749 - Psyr_1272 0.82 -0.8 1,438,779 - Psyr_1272 0.87 -0.9 1,438,779 - Psyr_1272 0.87 -1.4 1,438,779 - Psyr_1272 0.87 -0.8 1,438,779 - Psyr_1272 0.87 +0.5 1,439,012 + -1.6 1,439,012 + +1.1 1,439,013 - -0.1 1,439,013 - -0.9 1,439,013 - -0.1 1,439,013 - +0.7 1,439,013 - +0.2
Or see this region's nucleotide sequence