Experiment: KB with Chaulmoogric 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3000 and Psyr_3001 overlap by 32 nucleotides Psyr_3001 and Psyr_3002 are separated by 9 nucleotides Psyr_3002 and Psyr_3003 are separated by 376 nucleotides
Psyr_3000: Psyr_3000 - methyltransferase, putative, at 3,596,498 to 3,597,124
_3000
Psyr_3001: Psyr_3001 - Glycosyl transferase, family 2, at 3,597,093 to 3,597,746
_3001
Psyr_3002: Psyr_3002 - conserved hypothetical protein, at 3,597,756 to 3,598,796
_3002
Psyr_3003: Psyr_3003 - [Acyl-carrier protein] phosphodiesterase, at 3,599,173 to 3,599,781
_3003
Position (kb)
3597
3598
3599 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3596.846 kb on + strand, within Psyr_3000 at 3596.847 kb on - strand, within Psyr_3000 at 3597.158 kb on - strand at 3597.564 kb on + strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.757 kb on + strand at 3597.758 kb on - strand at 3597.971 kb on - strand, within Psyr_3002 at 3598.051 kb on + strand, within Psyr_3002 at 3598.051 kb on + strand, within Psyr_3002 at 3598.052 kb on - strand, within Psyr_3002 at 3598.052 kb on - strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.334 kb on - strand, within Psyr_3002 at 3598.706 kb on - strand at 3598.791 kb on + strand at 3598.791 kb on + strand at 3598.792 kb on - strand at 3598.792 kb on - strand at 3598.792 kb on - strand at 3598.894 kb on + strand at 3598.894 kb on + strand at 3598.894 kb on + strand at 3598.894 kb on + strand at 3598.895 kb on - strand at 3598.915 kb on + strand at 3598.916 kb on - strand at 3598.916 kb on - strand at 3598.934 kb on + strand at 3598.967 kb on - strand at 3598.967 kb on - strand at 3599.005 kb on - strand at 3599.010 kb on - strand at 3599.018 kb on - strand at 3599.035 kb on + strand at 3599.055 kb on + strand at 3599.055 kb on + strand at 3599.056 kb on - strand at 3599.148 kb on - strand at 3599.148 kb on - strand at 3599.201 kb on + strand at 3599.201 kb on + strand at 3599.202 kb on - strand at 3599.329 kb on + strand, within Psyr_3003 at 3599.495 kb on + strand, within Psyr_3003 at 3599.495 kb on + strand, within Psyr_3003 at 3599.495 kb on + strand, within Psyr_3003 at 3599.495 kb on + strand, within Psyr_3003 at 3599.496 kb on - strand, within Psyr_3003 at 3599.496 kb on - strand, within Psyr_3003 at 3599.496 kb on - strand, within Psyr_3003 at 3599.496 kb on - strand, within Psyr_3003 at 3599.496 kb on - strand, within Psyr_3003 at 3599.666 kb on + strand, within Psyr_3003 at 3599.699 kb on + strand, within Psyr_3003 at 3599.700 kb on - strand, within Psyr_3003 at 3599.774 kb on - strand at 3599.774 kb on - strand at 3599.786 kb on + strand at 3599.786 kb on + strand at 3599.787 kb on - strand at 3599.787 kb on - strand at 3599.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chaulmoogric 10 mM remove 3,596,846 + Psyr_3000 0.56 +0.9 3,596,847 - Psyr_3000 0.56 +1.7 3,597,158 - -0.0 3,597,564 + Psyr_3001 0.72 +0.3 3,597,564 + Psyr_3001 0.72 +0.1 3,597,564 + Psyr_3001 0.72 +1.8 3,597,565 - Psyr_3001 0.72 -1.8 3,597,565 - Psyr_3001 0.72 +0.2 3,597,565 - Psyr_3001 0.72 -0.0 3,597,565 - Psyr_3001 0.72 -0.6 3,597,565 - Psyr_3001 0.72 -1.5 3,597,757 + -1.6 3,597,758 - -0.4 3,597,971 - Psyr_3002 0.21 +0.4 3,598,051 + Psyr_3002 0.28 +0.1 3,598,051 + Psyr_3002 0.28 -1.1 3,598,052 - Psyr_3002 0.28 +0.4 3,598,052 - Psyr_3002 0.28 -0.7 3,598,329 + Psyr_3002 0.55 -1.8 3,598,329 + Psyr_3002 0.55 -1.9 3,598,329 + Psyr_3002 0.55 -0.5 3,598,329 + Psyr_3002 0.55 +1.5 3,598,330 - Psyr_3002 0.55 -0.0 3,598,330 - Psyr_3002 0.55 -0.6 3,598,330 - Psyr_3002 0.55 -0.0 3,598,330 - Psyr_3002 0.55 -0.2 3,598,330 - Psyr_3002 0.55 -0.5 3,598,330 - Psyr_3002 0.55 -0.5 3,598,330 - Psyr_3002 0.55 +1.0 3,598,334 - Psyr_3002 0.56 -0.6 3,598,706 - +0.4 3,598,791 + +0.1 3,598,791 + -0.2 3,598,792 - -0.7 3,598,792 - +3.0 3,598,792 - -0.3 3,598,894 + +0.5 3,598,894 + -0.9 3,598,894 + -1.2 3,598,894 + +0.0 3,598,895 - -0.2 3,598,915 + -0.0 3,598,916 - +0.6 3,598,916 - -3.8 3,598,934 + -0.3 3,598,967 - +0.5 3,598,967 - +1.8 3,599,005 - -0.6 3,599,010 - +0.8 3,599,018 - -2.3 3,599,035 + -2.1 3,599,055 + +1.3 3,599,055 + -3.0 3,599,056 - +0.4 3,599,148 - +1.0 3,599,148 - +1.2 3,599,201 + -1.1 3,599,201 + +0.4 3,599,202 - -0.0 3,599,329 + Psyr_3003 0.26 -0.2 3,599,495 + Psyr_3003 0.53 -0.8 3,599,495 + Psyr_3003 0.53 +1.4 3,599,495 + Psyr_3003 0.53 +1.0 3,599,495 + Psyr_3003 0.53 +0.1 3,599,496 - Psyr_3003 0.53 +1.4 3,599,496 - Psyr_3003 0.53 +1.5 3,599,496 - Psyr_3003 0.53 -1.7 3,599,496 - Psyr_3003 0.53 -0.0 3,599,496 - Psyr_3003 0.53 +0.5 3,599,666 + Psyr_3003 0.81 +0.7 3,599,699 + Psyr_3003 0.86 +1.4 3,599,700 - Psyr_3003 0.87 +0.3 3,599,774 - +0.2 3,599,774 - -1.3 3,599,786 + +1.4 3,599,786 + -1.0 3,599,787 - +3.3 3,599,787 - -2.5 3,599,787 - -0.4
Or see this region's nucleotide sequence