Experiment: KB with Chaulmoogric 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1363 and Psyr_1364 are separated by 112 nucleotides Psyr_1364 and Psyr_1365 are separated by 13 nucleotides Psyr_1365 and Psyr_1366 are separated by 7 nucleotides
Psyr_1363: Psyr_1363 - enolase, at 1,546,984 to 1,548,270
_1363
Psyr_1364: Psyr_1364 - cell division protein FtsB, at 1,548,383 to 1,548,739
_1364
Psyr_1365: Psyr_1365 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,548,753 to 1,549,463
_1365
Psyr_1366: Psyr_1366 - transcriptional regulator, LysR family, at 1,549,471 to 1,550,367
_1366
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1547.756 kb on - strand, within Psyr_1363 at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.275 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.382 kb on + strand at 1548.472 kb on + strand, within Psyr_1364 at 1548.737 kb on + strand at 1549.432 kb on + strand at 1549.482 kb on - strand at 1549.515 kb on - strand at 1549.646 kb on + strand, within Psyr_1366 at 1549.646 kb on + strand, within Psyr_1366 at 1549.647 kb on - strand, within Psyr_1366 at 1549.649 kb on + strand, within Psyr_1366 at 1549.666 kb on + strand, within Psyr_1366 at 1549.666 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.714 kb on - strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1550.009 kb on + strand, within Psyr_1366 at 1550.010 kb on - strand, within Psyr_1366 at 1550.214 kb on - strand, within Psyr_1366 at 1550.364 kb on - strand at 1550.364 kb on - strand at 1550.380 kb on + strand at 1550.381 kb on - strand at 1550.431 kb on - strand at 1550.433 kb on + strand at 1550.433 kb on + strand at 1550.433 kb on + strand at 1550.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chaulmoogric 10 mM remove 1,547,756 - Psyr_1363 0.60 +1.8 1,548,275 + +1.3 1,548,275 + -0.2 1,548,275 + +0.2 1,548,275 + +0.8 1,548,275 + -1.7 1,548,275 + +0.2 1,548,275 + -0.4 1,548,275 + -1.3 1,548,275 + -0.5 1,548,275 + -0.7 1,548,275 + -0.8 1,548,382 + +1.9 1,548,382 + +1.5 1,548,382 + +0.1 1,548,382 + +1.0 1,548,382 + +1.0 1,548,382 + -1.8 1,548,472 + Psyr_1364 0.25 +0.0 1,548,737 + +0.3 1,549,432 + +0.0 1,549,482 - +0.5 1,549,515 - -1.1 1,549,646 + Psyr_1366 0.20 -0.3 1,549,646 + Psyr_1366 0.20 +2.4 1,549,647 - Psyr_1366 0.20 -0.8 1,549,649 + Psyr_1366 0.20 +0.4 1,549,666 + Psyr_1366 0.22 -1.2 1,549,666 + Psyr_1366 0.22 -0.2 1,549,713 + Psyr_1366 0.27 +1.4 1,549,713 + Psyr_1366 0.27 +1.0 1,549,713 + Psyr_1366 0.27 -1.0 1,549,714 - Psyr_1366 0.27 +1.6 1,549,850 + Psyr_1366 0.42 +0.6 1,549,850 + Psyr_1366 0.42 -0.8 1,549,850 + Psyr_1366 0.42 -0.6 1,549,851 - Psyr_1366 0.42 +0.1 1,549,851 - Psyr_1366 0.42 +0.9 1,549,851 - Psyr_1366 0.42 +2.3 1,549,851 - Psyr_1366 0.42 +0.7 1,549,851 - Psyr_1366 0.42 +0.5 1,549,871 + Psyr_1366 0.45 +1.4 1,549,871 + Psyr_1366 0.45 +0.4 1,549,871 + Psyr_1366 0.45 +0.6 1,549,871 + Psyr_1366 0.45 +0.7 1,549,871 + Psyr_1366 0.45 +0.2 1,549,871 + Psyr_1366 0.45 +0.9 1,549,871 + Psyr_1366 0.45 +2.0 1,549,872 - Psyr_1366 0.45 +0.6 1,549,872 - Psyr_1366 0.45 +2.6 1,549,872 - Psyr_1366 0.45 +0.7 1,550,009 + Psyr_1366 0.60 -0.5 1,550,010 - Psyr_1366 0.60 -0.8 1,550,214 - Psyr_1366 0.83 -0.2 1,550,364 - +1.1 1,550,364 - +2.9 1,550,380 + -0.2 1,550,381 - -0.5 1,550,431 - -0.3 1,550,433 + -0.7 1,550,433 + +0.1 1,550,433 + -0.8 1,550,434 - +0.1
Or see this region's nucleotide sequence