Experiment: D-Lysine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0383 and PP_0384 are separated by 253 nucleotides PP_0384 and PP_0385 are separated by 187 nucleotides PP_0385 and PP_5445 are separated by 343 nucleotides PP_5445 and PP_t71 are separated by 52 nucleotides PP_t71 and PP_0386 are separated by 16 nucleotides
PP_0383: PP_0383 - Lysine 2-monooxygenase, at 463,414 to 465,096
_0383
PP_0384: PP_0384 - Transcriptional regulator, AsnC family, at 465,350 to 465,811
_0384
PP_0385: PP_0385 - putative serine acetyltransferase, at 465,999 to 466,553
_0385
PP_5445: PP_5445 - conserved protein of unknown function, at 466,897 to 467,067
_5445
PP_t71: PP_t71 - tRNA-Met, at 467,120 to 467,196
_t71
PP_0386: PP_0386 - Sensory box protein, at 467,213 to 470,956
_0386
Position (kb)
465
466
467 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 465.141 kb on + strand at 465.143 kb on + strand at 465.143 kb on + strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.166 kb on + strand at 465.167 kb on - strand at 465.184 kb on + strand at 465.188 kb on + strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.214 kb on - strand at 465.241 kb on + strand at 465.242 kb on - strand at 465.271 kb on + strand at 465.271 kb on + strand at 465.271 kb on + strand at 465.272 kb on - strand at 465.307 kb on + strand at 465.308 kb on - strand at 465.486 kb on + strand, within PP_0384 at 465.486 kb on + strand, within PP_0384 at 465.487 kb on - strand, within PP_0384 at 465.487 kb on - strand, within PP_0384 at 465.487 kb on - strand at 465.504 kb on - strand, within PP_0384 at 465.591 kb on + strand, within PP_0384 at 465.591 kb on + strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.632 kb on + strand, within PP_0384 at 465.640 kb on - strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.823 kb on - strand at 465.841 kb on + strand at 465.874 kb on + strand at 465.874 kb on + strand at 465.875 kb on - strand at 465.875 kb on - strand at 465.875 kb on - strand at 466.067 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.218 kb on + strand, within PP_0385 at 466.299 kb on + strand, within PP_0385 at 466.300 kb on - strand, within PP_0385 at 466.374 kb on + strand, within PP_0385 at 466.375 kb on - strand, within PP_0385 at 466.456 kb on + strand, within PP_0385 at 466.457 kb on - strand, within PP_0385 at 466.512 kb on + strand at 466.564 kb on + strand at 466.565 kb on - strand at 466.565 kb on - strand at 466.616 kb on + strand at 466.616 kb on + strand at 466.688 kb on - strand at 466.728 kb on - strand at 466.756 kb on + strand at 466.865 kb on + strand at 466.865 kb on + strand at 466.903 kb on + strand at 466.904 kb on - strand at 466.904 kb on - strand at 466.922 kb on + strand, within PP_5445 at 466.923 kb on - strand, within PP_5445 at 467.006 kb on - strand, within PP_5445 at 467.051 kb on + strand at 467.051 kb on + strand at 467.052 kb on - strand at 467.070 kb on - strand at 467.202 kb on - strand at 467.202 kb on - strand at 467.215 kb on - strand at 467.215 kb on - strand at 467.217 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.520 kb on + strand at 467.521 kb on - strand at 467.521 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lysine 10 mM (C) remove 465,141 + +0.5 465,143 + -1.7 465,143 + -1.0 465,144 - -0.2 465,144 - -0.9 465,144 - +1.5 465,166 + -0.1 465,167 - -0.6 465,184 + -0.2 465,188 + +1.4 465,189 - -1.1 465,189 - -1.3 465,189 - -0.6 465,189 - +0.5 465,214 - +0.2 465,241 + -0.8 465,242 - -2.9 465,271 + -1.5 465,271 + -0.6 465,271 + -1.1 465,272 - +0.2 465,307 + +0.1 465,308 - -0.2 465,486 + PP_0384 0.29 -2.3 465,486 + PP_0384 0.29 +0.6 465,487 - PP_0384 0.30 -4.2 465,487 - PP_0384 0.30 +1.2 465,487 - -0.5 465,504 - PP_0384 0.33 -0.9 465,591 + PP_0384 0.52 -2.4 465,591 + PP_0384 0.52 -1.6 465,592 - PP_0384 0.52 +0.2 465,592 - PP_0384 0.52 -2.7 465,592 - PP_0384 0.52 -2.8 465,592 - PP_0384 0.52 -1.3 465,632 + PP_0384 0.61 -0.5 465,640 - PP_0384 0.63 -3.6 465,649 + PP_0384 0.65 -0.9 465,649 + PP_0384 0.65 -3.9 465,649 + PP_0384 0.65 +0.0 465,649 + PP_0384 0.65 -3.8 465,650 - PP_0384 0.65 -0.4 465,650 - PP_0384 0.65 -2.8 465,650 - PP_0384 0.65 -0.2 465,823 - +0.0 465,841 + -0.2 465,874 + +1.7 465,874 + +0.9 465,875 - -0.1 465,875 - -0.6 465,875 - +1.1 466,067 + PP_0385 0.12 -1.4 466,149 + PP_0385 0.27 -1.2 466,149 + PP_0385 0.27 -0.5 466,218 + PP_0385 0.39 +0.5 466,299 + PP_0385 0.54 -0.1 466,300 - PP_0385 0.54 +1.2 466,374 + PP_0385 0.68 -1.0 466,375 - PP_0385 0.68 -0.5 466,456 + PP_0385 0.82 +0.6 466,457 - PP_0385 0.83 +0.8 466,512 + +1.6 466,564 + -0.9 466,565 - +0.9 466,565 - -0.2 466,616 + -0.0 466,616 + -0.4 466,688 - -0.7 466,728 - +0.5 466,756 + +1.2 466,865 + -0.1 466,865 + +0.4 466,903 + +0.2 466,904 - -1.1 466,904 - +1.8 466,922 + PP_5445 0.15 -4.1 466,923 - PP_5445 0.15 -0.0 467,006 - PP_5445 0.64 +0.7 467,051 + +0.3 467,051 + +0.8 467,052 - +0.3 467,070 - +0.6 467,202 - -1.1 467,202 - -0.1 467,215 - +0.5 467,215 - +1.7 467,217 - -3.6 467,262 - +1.9 467,262 - +0.1 467,262 - +0.7 467,317 - -1.1 467,317 - +0.3 467,317 - -0.7 467,520 + -0.4 467,521 - +1.0 467,521 - -0.7
Or see this region's nucleotide sequence