Experiment: KB with Sclareolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2392 and Psyr_2393 are separated by 141 nucleotides Psyr_2393 and Psyr_2394 are separated by 233 nucleotides Psyr_2394 and Psyr_2395 are separated by 101 nucleotides Psyr_2395 and Psyr_2396 overlap by 4 nucleotides
Psyr_2392: Psyr_2392 - conserved hypothetical protein, at 2,774,106 to 2,774,729
_2392
Psyr_2393: Psyr_2393 - Flavin reductase-like protein, at 2,774,871 to 2,775,455
_2393
Psyr_2394: Psyr_2394 - succinate semialdehyde dehydrogenase, at 2,775,689 to 2,777,080
_2394
Psyr_2395: Psyr_2395 - transcriptional regulator, LysR family, at 2,777,182 to 2,778,057
_2395
Psyr_2396: Psyr_2396 - Tartrate dehydrogenase, at 2,778,054 to 2,779,175
_2396
Position (kb)
2775
2776
2777
2778 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2774.740 kb on + strand at 2774.740 kb on + strand at 2774.882 kb on + strand at 2774.883 kb on - strand at 2775.078 kb on + strand, within Psyr_2393 at 2775.282 kb on + strand, within Psyr_2393 at 2775.282 kb on + strand, within Psyr_2393 at 2775.282 kb on + strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.283 kb on - strand, within Psyr_2393 at 2775.390 kb on + strand, within Psyr_2393 at 2775.391 kb on - strand, within Psyr_2393 at 2775.412 kb on - strand at 2775.413 kb on + strand at 2775.414 kb on - strand at 2775.675 kb on + strand at 2775.675 kb on + strand at 2775.676 kb on - strand at 2775.676 kb on - strand at 2775.813 kb on + strand at 2775.886 kb on - strand, within Psyr_2394 at 2776.041 kb on + strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.042 kb on - strand, within Psyr_2394 at 2776.162 kb on - strand, within Psyr_2394 at 2776.162 kb on - strand, within Psyr_2394 at 2776.569 kb on + strand, within Psyr_2394 at 2776.583 kb on + strand, within Psyr_2394 at 2776.583 kb on + strand, within Psyr_2394 at 2776.584 kb on - strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.608 kb on + strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2776.609 kb on - strand, within Psyr_2394 at 2777.011 kb on - strand at 2777.074 kb on + strand at 2777.075 kb on - strand at 2777.075 kb on - strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.119 kb on - strand at 2777.119 kb on - strand at 2777.119 kb on - strand at 2777.380 kb on + strand, within Psyr_2395 at 2777.512 kb on + strand, within Psyr_2395 at 2777.513 kb on - strand, within Psyr_2395 at 2777.592 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.834 kb on - strand, within Psyr_2395 at 2777.834 kb on - strand, within Psyr_2395
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sclareolide 10 mM remove 2,774,740 + +1.9 2,774,740 + +0.9 2,774,882 + +0.3 2,774,883 - +2.0 2,775,078 + Psyr_2393 0.35 -1.8 2,775,282 + Psyr_2393 0.70 +0.8 2,775,282 + Psyr_2393 0.70 -0.3 2,775,282 + Psyr_2393 0.70 -0.6 2,775,283 - Psyr_2393 0.70 -0.5 2,775,283 - Psyr_2393 0.70 -0.7 2,775,283 - Psyr_2393 0.70 -1.8 2,775,283 - Psyr_2393 0.70 +0.1 2,775,390 + Psyr_2393 0.89 +0.5 2,775,391 - Psyr_2393 0.89 +2.1 2,775,412 - -1.3 2,775,413 + -0.2 2,775,414 - +0.3 2,775,675 + +0.7 2,775,675 + +0.9 2,775,676 - +1.7 2,775,676 - +0.4 2,775,813 + +0.1 2,775,886 - Psyr_2394 0.14 +0.9 2,776,041 + Psyr_2394 0.25 +1.3 2,776,042 - Psyr_2394 0.25 +0.1 2,776,042 - Psyr_2394 0.25 +0.5 2,776,042 - Psyr_2394 0.25 +0.2 2,776,042 - Psyr_2394 0.25 +0.6 2,776,162 - Psyr_2394 0.34 +0.4 2,776,162 - Psyr_2394 0.34 +0.3 2,776,569 + Psyr_2394 0.63 -1.5 2,776,583 + Psyr_2394 0.64 +0.7 2,776,583 + Psyr_2394 0.64 +0.5 2,776,584 - Psyr_2394 0.64 -0.8 2,776,608 + Psyr_2394 0.66 +0.5 2,776,608 + Psyr_2394 0.66 +0.8 2,776,608 + Psyr_2394 0.66 -0.5 2,776,608 + Psyr_2394 0.66 -0.8 2,776,608 + Psyr_2394 0.66 -1.0 2,776,609 - Psyr_2394 0.66 +3.2 2,776,609 - Psyr_2394 0.66 -2.1 2,776,609 - Psyr_2394 0.66 +2.5 2,776,609 - Psyr_2394 0.66 +3.3 2,776,609 - Psyr_2394 0.66 +0.6 2,776,609 - Psyr_2394 0.66 +1.9 2,776,609 - Psyr_2394 0.66 -1.0 2,777,011 - -0.1 2,777,074 + +0.4 2,777,075 - -0.1 2,777,075 - -1.1 2,777,116 + -2.3 2,777,116 + -0.6 2,777,118 + +0.6 2,777,118 + -0.5 2,777,118 + +0.1 2,777,118 + -1.1 2,777,119 - +4.1 2,777,119 - -0.1 2,777,119 - -0.9 2,777,380 + Psyr_2395 0.23 +2.4 2,777,512 + Psyr_2395 0.38 +2.4 2,777,513 - Psyr_2395 0.38 +1.8 2,777,592 + Psyr_2395 0.47 -0.3 2,777,733 + Psyr_2395 0.63 +0.8 2,777,733 + Psyr_2395 0.63 -1.6 2,777,733 + Psyr_2395 0.63 +0.6 2,777,733 + Psyr_2395 0.63 +0.5 2,777,733 + Psyr_2395 0.63 +0.3 2,777,734 - Psyr_2395 0.63 -0.2 2,777,734 - Psyr_2395 0.63 +0.9 2,777,734 - Psyr_2395 0.63 -0.9 2,777,734 - Psyr_2395 0.63 -2.0 2,777,734 - Psyr_2395 0.63 +2.5 2,777,734 - Psyr_2395 0.63 -2.9 2,777,738 - Psyr_2395 0.63 -0.1 2,777,738 - Psyr_2395 0.63 +0.8 2,777,738 - Psyr_2395 0.63 -1.6 2,777,834 - Psyr_2395 0.74 -1.5 2,777,834 - Psyr_2395 0.74 -0.4
Or see this region's nucleotide sequence