Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2168

Experiment: KB with Sclareolide 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2167 and Psyr_2168 are separated by 3 nucleotidesPsyr_2168 and Psyr_2169 overlap by 20 nucleotidesPsyr_2169 and Psyr_RNA40 are separated by 88 nucleotidesPsyr_RNA40 and Psyr_2170 are separated by 158 nucleotides Psyr_2167: Psyr_2167 - phenylalanyl-tRNA synthetase beta subunit, at 2,519,998 to 2,522,376 _2167 Psyr_2168: Psyr_2168 - Integration host factor, alpha subunit, at 2,522,380 to 2,522,682 _2168 Psyr_2169: Psyr_2169 - transcriptional regulator, MerR family, at 2,522,663 to 2,523,019 _2169 Psyr_RNA40: Psyr_RNA40 - tRNA-Pro, at 2,523,108 to 2,523,184 _RNA40 Psyr_2170: Psyr_2170 - L-glutamate-binding protein / L-aspartate-binding protein, at 2,523,343 to 2,524,257 _2170 Position (kb) 2522 2523Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2522.420 kb on - strand, within Psyr_2168at 2522.662 kb on + strandat 2522.662 kb on + strandat 2522.662 kb on + strandat 2522.662 kb on + strandat 2522.662 kb on + strandat 2522.663 kb on - strandat 2522.663 kb on - strandat 2522.663 kb on - strandat 2522.663 kb on - strandat 2522.663 kb on - strandat 2522.714 kb on + strand, within Psyr_2169at 2522.714 kb on + strand, within Psyr_2169at 2522.715 kb on - strand, within Psyr_2169at 2522.715 kb on - strand, within Psyr_2169at 2522.828 kb on + strand, within Psyr_2169at 2522.877 kb on + strand, within Psyr_2169at 2522.877 kb on + strand, within Psyr_2169at 2522.879 kb on + strand, within Psyr_2169at 2522.879 kb on + strand, within Psyr_2169at 2522.880 kb on - strand, within Psyr_2169at 2522.880 kb on - strand, within Psyr_2169at 2522.920 kb on + strand, within Psyr_2169at 2522.955 kb on - strand, within Psyr_2169at 2522.955 kb on - strand, within Psyr_2169at 2522.998 kb on - strandat 2523.017 kb on + strandat 2523.017 kb on + strandat 2523.034 kb on + strandat 2523.060 kb on + strandat 2523.063 kb on - strandat 2523.097 kb on - strandat 2523.136 kb on + strand, within Psyr_RNA40at 2523.137 kb on - strand, within Psyr_RNA40at 2523.185 kb on + strandat 2523.188 kb on - strandat 2523.210 kb on + strandat 2523.288 kb on - strandat 2523.453 kb on + strand, within Psyr_2170at 2523.453 kb on + strand, within Psyr_2170at 2523.462 kb on + strand, within Psyr_2170at 2523.463 kb on - strand, within Psyr_2170at 2523.467 kb on + strand, within Psyr_2170at 2523.467 kb on + strand, within Psyr_2170at 2523.468 kb on - strand, within Psyr_2170at 2523.468 kb on - strand, within Psyr_2170at 2523.505 kb on - strand, within Psyr_2170at 2523.563 kb on + strand, within Psyr_2170at 2523.563 kb on + strand, within Psyr_2170at 2523.658 kb on - strand, within Psyr_2170at 2523.658 kb on - strand, within Psyr_2170

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Sclareolide 10 mM
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2,522,420 - Psyr_2168 0.13 +0.5
2,522,662 + +0.6
2,522,662 + -1.2
2,522,662 + +2.1
2,522,662 + -1.3
2,522,662 + +0.6
2,522,663 - -0.6
2,522,663 - -0.6
2,522,663 - -0.8
2,522,663 - +1.0
2,522,663 - +2.2
2,522,714 + Psyr_2169 0.14 +1.3
2,522,714 + Psyr_2169 0.14 +0.4
2,522,715 - Psyr_2169 0.15 -0.0
2,522,715 - Psyr_2169 0.15 +0.2
2,522,828 + Psyr_2169 0.46 +1.2
2,522,877 + Psyr_2169 0.60 -0.6
2,522,877 + Psyr_2169 0.60 +1.0
2,522,879 + Psyr_2169 0.61 +0.1
2,522,879 + Psyr_2169 0.61 +2.2
2,522,880 - Psyr_2169 0.61 +1.1
2,522,880 - Psyr_2169 0.61 +2.4
2,522,920 + Psyr_2169 0.72 +1.0
2,522,955 - Psyr_2169 0.82 -0.8
2,522,955 - Psyr_2169 0.82 +0.4
2,522,998 - +0.2
2,523,017 + +1.2
2,523,017 + -0.3
2,523,034 + +0.2
2,523,060 + +0.9
2,523,063 - +1.3
2,523,097 - -1.9
2,523,136 + Psyr_RNA40 0.36 +0.0
2,523,137 - Psyr_RNA40 0.38 -1.5
2,523,185 + +1.0
2,523,188 - -2.4
2,523,210 + -1.4
2,523,288 - -0.0
2,523,453 + Psyr_2170 0.12 -1.4
2,523,453 + Psyr_2170 0.12 -1.6
2,523,462 + Psyr_2170 0.13 -3.9
2,523,463 - Psyr_2170 0.13 +0.5
2,523,467 + Psyr_2170 0.14 +0.3
2,523,467 + Psyr_2170 0.14 +0.0
2,523,468 - Psyr_2170 0.14 +1.0
2,523,468 - Psyr_2170 0.14 -2.2
2,523,505 - Psyr_2170 0.18 +0.4
2,523,563 + Psyr_2170 0.24 -2.2
2,523,563 + Psyr_2170 0.24 -1.3
2,523,658 - Psyr_2170 0.34 -0.5
2,523,658 - Psyr_2170 0.34 -0.7

Or see this region's nucleotide sequence