Experiment: KB with Sclareolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1727 and Psyr_1728 are separated by 84 nucleotides Psyr_1728 and Psyr_1729 are separated by 149 nucleotides Psyr_1729 and Psyr_1730 are separated by 72 nucleotides
Psyr_1727: Psyr_1727 - Protein of unknown function DUF1289, at 1,961,923 to 1,962,393
_1727
Psyr_1728: Psyr_1728 - tRNA--hydroxylase, at 1,962,478 to 1,963,089
_1728
Psyr_1729: Psyr_1729 - transcriptional regulator, MarR family, at 1,963,239 to 1,963,727
_1729
Psyr_1730: Psyr_1730 - Secretion protein HlyD, at 1,963,800 to 1,965,008
_1730
Position (kb)
1963
1964 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1962.352 kb on + strand at 1962.352 kb on + strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.438 kb on + strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.441 kb on - strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.482 kb on - strand at 1962.482 kb on - strand at 1962.632 kb on - strand, within Psyr_1728 at 1962.744 kb on + strand, within Psyr_1728 at 1962.907 kb on + strand, within Psyr_1728 at 1962.907 kb on + strand, within Psyr_1728 at 1962.908 kb on - strand, within Psyr_1728 at 1962.908 kb on - strand, within Psyr_1728 at 1962.949 kb on + strand, within Psyr_1728 at 1962.964 kb on + strand, within Psyr_1728 at 1963.073 kb on + strand at 1963.150 kb on + strand at 1963.151 kb on - strand at 1963.153 kb on + strand at 1963.153 kb on + strand at 1963.154 kb on - strand at 1963.154 kb on - strand at 1963.165 kb on + strand at 1963.216 kb on + strand at 1963.224 kb on + strand at 1963.225 kb on - strand at 1963.332 kb on + strand, within Psyr_1729 at 1963.332 kb on + strand, within Psyr_1729 at 1963.332 kb on + strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.344 kb on + strand, within Psyr_1729 at 1963.344 kb on + strand, within Psyr_1729 at 1963.351 kb on + strand, within Psyr_1729 at 1963.351 kb on + strand, within Psyr_1729 at 1963.352 kb on - strand, within Psyr_1729 at 1963.718 kb on + strand at 1963.792 kb on + strand at 1963.793 kb on - strand at 1963.793 kb on - strand at 1963.941 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1964.030 kb on + strand, within Psyr_1730 at 1964.163 kb on + strand, within Psyr_1730 at 1964.164 kb on - strand, within Psyr_1730 at 1964.165 kb on + strand, within Psyr_1730 at 1964.166 kb on - strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.237 kb on + strand, within Psyr_1730 at 1964.337 kb on - strand, within Psyr_1730 at 1964.337 kb on - strand, within Psyr_1730 at 1964.437 kb on - strand, within Psyr_1730 at 1964.482 kb on - strand, within Psyr_1730 at 1964.482 kb on - strand, within Psyr_1730 at 1964.644 kb on - strand, within Psyr_1730 at 1964.644 kb on - strand, within Psyr_1730 at 1964.661 kb on + strand, within Psyr_1730 at 1964.661 kb on + strand, within Psyr_1730 at 1964.662 kb on - strand, within Psyr_1730 at 1964.662 kb on - strand, within Psyr_1730
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sclareolide 10 mM remove 1,962,352 + +0.9 1,962,352 + -0.3 1,962,353 - -0.5 1,962,353 - -0.6 1,962,353 - -0.9 1,962,438 + -0.3 1,962,439 - -1.1 1,962,439 - +0.9 1,962,439 - -0.8 1,962,441 - +1.4 1,962,477 + -0.2 1,962,477 + +0.5 1,962,477 + +0.0 1,962,477 + +1.5 1,962,477 + +0.2 1,962,478 - -0.3 1,962,478 - +1.3 1,962,482 - +0.9 1,962,482 - +0.3 1,962,632 - Psyr_1728 0.25 -0.4 1,962,744 + Psyr_1728 0.43 -1.9 1,962,907 + Psyr_1728 0.70 -0.5 1,962,907 + Psyr_1728 0.70 +0.5 1,962,908 - Psyr_1728 0.70 +0.1 1,962,908 - Psyr_1728 0.70 +0.2 1,962,949 + Psyr_1728 0.77 +0.8 1,962,964 + Psyr_1728 0.79 +0.9 1,963,073 + -1.3 1,963,150 + -1.1 1,963,151 - -1.2 1,963,153 + +1.2 1,963,153 + -0.1 1,963,154 - +0.8 1,963,154 - -2.4 1,963,165 + -1.2 1,963,216 + +0.6 1,963,224 + -0.7 1,963,225 - +0.8 1,963,332 + Psyr_1729 0.19 -1.4 1,963,332 + Psyr_1729 0.19 -0.1 1,963,332 + Psyr_1729 0.19 +1.2 1,963,333 - Psyr_1729 0.19 +0.7 1,963,333 - Psyr_1729 0.19 -2.1 1,963,333 - Psyr_1729 0.19 -0.0 1,963,333 - Psyr_1729 0.19 +1.3 1,963,344 + Psyr_1729 0.21 -1.4 1,963,344 + Psyr_1729 0.21 +1.0 1,963,351 + Psyr_1729 0.23 -0.6 1,963,351 + Psyr_1729 0.23 -0.7 1,963,352 - Psyr_1729 0.23 +0.7 1,963,718 + -0.3 1,963,792 + +1.5 1,963,793 - +0.2 1,963,793 - -3.9 1,963,941 + Psyr_1730 0.12 +0.0 1,963,974 + Psyr_1730 0.14 +0.2 1,963,974 + Psyr_1730 0.14 +1.2 1,963,974 + Psyr_1730 0.14 -0.5 1,963,974 + Psyr_1730 0.14 +1.1 1,963,975 - Psyr_1730 0.14 -0.8 1,963,975 - Psyr_1730 0.14 +0.7 1,964,030 + Psyr_1730 0.19 -0.1 1,964,163 + Psyr_1730 0.30 -1.5 1,964,164 - Psyr_1730 0.30 -0.1 1,964,165 + Psyr_1730 0.30 -0.3 1,964,166 - Psyr_1730 0.30 +1.4 1,964,235 + Psyr_1730 0.36 +0.4 1,964,235 + Psyr_1730 0.36 -1.3 1,964,235 + Psyr_1730 0.36 +0.8 1,964,235 + Psyr_1730 0.36 +0.8 1,964,235 + Psyr_1730 0.36 +0.7 1,964,235 + Psyr_1730 0.36 +0.0 1,964,236 - Psyr_1730 0.36 +0.3 1,964,236 - Psyr_1730 0.36 +3.4 1,964,236 - Psyr_1730 0.36 -1.6 1,964,236 - Psyr_1730 0.36 +2.2 1,964,236 - Psyr_1730 0.36 +0.8 1,964,236 - Psyr_1730 0.36 -0.2 1,964,236 - Psyr_1730 0.36 +2.8 1,964,236 - Psyr_1730 0.36 +0.2 1,964,236 - Psyr_1730 0.36 -0.9 1,964,236 - Psyr_1730 0.36 -0.4 1,964,236 - Psyr_1730 0.36 +0.4 1,964,236 - Psyr_1730 0.36 -0.8 1,964,236 - Psyr_1730 0.36 -3.0 1,964,236 - Psyr_1730 0.36 -0.7 1,964,237 + Psyr_1730 0.36 -3.5 1,964,337 - Psyr_1730 0.44 -0.6 1,964,337 - Psyr_1730 0.44 -0.6 1,964,437 - Psyr_1730 0.53 -0.8 1,964,482 - Psyr_1730 0.56 +0.6 1,964,482 - Psyr_1730 0.56 -1.5 1,964,644 - Psyr_1730 0.70 -1.4 1,964,644 - Psyr_1730 0.70 +0.6 1,964,661 + Psyr_1730 0.71 -0.3 1,964,661 + Psyr_1730 0.71 +0.3 1,964,662 - Psyr_1730 0.71 +0.7 1,964,662 - Psyr_1730 0.71 -0.3
Or see this region's nucleotide sequence