Experiment: BC Lignin Sorghum 10 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0438 and PP_0439 overlap by 32 nucleotides PP_0439 and PP_t01 are separated by 152 nucleotides PP_t01 and PP_t02 are separated by 24 nucleotides PP_t02 and PP_t03 are separated by 29 nucleotides PP_t03 and PP_0440 are separated by 78 nucleotides PP_0440 and PP_t04 are separated by 54 nucleotides PP_t04 and PP_0441 are separated by 44 nucleotides PP_0441 and PP_0442 are separated by 9 nucleotides
PP_0438: PP_0438 - Type III pantothenate kinase, at 531,036 to 531,785
_0438
PP_0439: PP_0439 - conserved protein of unknown function, at 531,754 to 532,218
_0439
PP_t01: PP_t01 - tRNA-Tyr, at 532,371 to 532,454
_t01
PP_t02: PP_t02 - tRNA-Gly, at 532,479 to 532,552
_t02
PP_t03: PP_t03 - tRNA-Thr, at 532,582 to 532,657
_t03
PP_0440: PP_0440 - Elongation factor Tu-A, at 532,736 to 533,929
_0440
PP_t04: PP_t04 - tRNA-Trp, at 533,984 to 534,059
_t04
PP_0441: PP_0441 - Sec translocation complex - SecE subunit, at 534,104 to 534,472
_0441
PP_0442: PP_0442 - transcription antipausing factor NusG, at 534,482 to 535,015
_0442
Position (kb) 532
533
534 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 531.792 kb on + strand at 531.792 kb on + strand at 531.793 kb on - strand at 531.815 kb on + strand, within PP_0439 at 531.836 kb on - strand, within PP_0439 at 531.836 kb on - strand, within PP_0439 at 531.838 kb on + strand, within PP_0439 at 531.839 kb on - strand, within PP_0439 at 531.839 kb on - strand, within PP_0439 at 531.842 kb on + strand, within PP_0439 at 531.842 kb on + strand, within PP_0439 at 531.843 kb on - strand, within PP_0439 at 531.843 kb on - strand, within PP_0439 at 531.898 kb on + strand, within PP_0439 at 531.899 kb on - strand, within PP_0439 at 531.899 kb on - strand, within PP_0439 at 532.172 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.660 kb on + strand at 532.710 kb on + strand at 532.742 kb on - strand at 533.263 kb on + strand, within PP_0440 at 533.302 kb on + strand, within PP_0440 at 533.311 kb on + strand, within PP_0440 at 533.312 kb on - strand, within PP_0440 at 533.910 kb on + strand at 533.927 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BC Lignin Sorghum 10 vol% (C) remove 531,792 + -1.3 531,792 + +1.0 531,793 - -0.5 531,815 + PP_0439 0.13 +0.1 531,836 - PP_0439 0.18 +1.5 531,836 - PP_0439 0.18 +2.4 531,838 + PP_0439 0.18 +0.2 531,839 - PP_0439 0.18 +0.6 531,839 - PP_0439 0.18 -1.3 531,842 + PP_0439 0.19 +1.1 531,842 + PP_0439 0.19 -2.1 531,843 - PP_0439 0.19 +0.4 531,843 - PP_0439 0.19 -1.0 531,898 + PP_0439 0.31 -0.8 531,899 - PP_0439 0.31 -0.6 531,899 - PP_0439 0.31 -0.8 532,172 + -0.2 532,658 + +4.7 532,658 + -2.3 532,658 + +1.6 532,658 + -0.9 532,658 + -1.3 532,658 + +0.4 532,660 + -0.8 532,710 + +0.6 532,742 - +0.5 533,263 + PP_0440 0.44 -1.8 533,302 + PP_0440 0.47 -2.0 533,311 + PP_0440 0.48 -0.3 533,312 - PP_0440 0.48 -1.5 533,910 + -0.5 533,927 + -0.4
Or see this region's nucleotide sequence