Strain Fitness in Pseudomonas putida KT2440 around PP_5647

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
4,423,720	+		PP_5646	0.81	+1.6
4,423,760	+				-0.7
4,423,760	+				-0.7
4,423,761	-				-0.6
4,423,761	-				-0.5
4,423,942	+		PP_3915	0.54	+1.3
4,423,942	+		PP_3915	0.54	+0.5
4,423,942	+		PP_3915	0.54	+1.7
4,423,943	-		PP_3915	0.54	+1.0
4,423,981	+		PP_3915	0.66	-0.2
4,423,981	+		PP_3915	0.66	-0.7
4,423,981	+		PP_3915	0.66	-1.0
4,423,981	+		PP_3915	0.66	-1.7
4,423,982	-		PP_3915	0.66	-0.5
4,423,982	-		PP_3915	0.66	-0.8
4,423,982	-		PP_3915	0.66	-0.4
4,424,032	+		PP_3915	0.81	-0.2
4,424,062	+				+1.7
4,424,062	+				-0.8
4,424,062	+				-2.3
4,424,062	+				+2.0
4,424,063	-				+1.0
4,424,224	-		PP_3916	0.35	-0.6
4,424,344	-		PP_3916	0.65	+0.9
4,424,425	+		PP_3916	0.86	-3.6
4,424,426	-		PP_3916	0.86	-0.2
4,424,501	+				-0.5
4,424,501	+				+0.1
4,424,501	+				-0.9
4,424,502	-				-0.8
4,424,502	-				-0.6
4,424,502	-				+0.5
4,424,502	-				+1.7
4,424,502	-				-0.9
4,424,510	+				+0.8
4,424,510	+				-2.8
4,424,511	-				-0.4
4,424,511	-				+0.8
4,424,519	+				+0.4
4,424,519	+				+0.7
4,424,520	-				+0.3
4,424,660	-				+0.3
4,424,660	-				-0.7
4,424,672	-				+1.9
4,424,735	+		PP_5647	0.24	+0.7
4,424,735	+		PP_5647	0.24	-1.4
4,424,735	+		PP_5647	0.24	+1.0
4,424,736	-		PP_5647	0.24	-1.2
4,424,758	+		PP_5647	0.32	-1.1
4,424,759	-		PP_5647	0.33	-1.2
4,424,759	-		PP_5647	0.33	-0.2
4,424,794	+		PP_5647	0.45	+1.1
4,424,794	+		PP_5647	0.45	-0.4
4,424,794	+		PP_5647	0.45	-1.1
4,424,795	-		PP_5647	0.46	-0.6
4,424,795	-		PP_5647	0.46	-3.3
4,424,942	+				-0.1
4,424,942	+				-0.9
4,424,942	+				+0.5
4,424,942	+				-0.9
4,424,942	+				+0.4
4,424,943	-				-1.8
4,424,943	-				+1.2
4,424,943	-				+0.7
4,424,943	-				-1.1
4,424,956	+				+0.2
4,424,956	+				+0.2
4,424,956	+				+1.0
4,424,956	+				-0.1
4,424,956	+				+0.2
4,424,956	+				-1.7
4,424,956	+				+0.8
4,424,956	+				+0.8
4,424,956	+				+1.1
4,424,957	-				-2.3
4,424,957	-				+0.2
4,424,957	-				-0.5
4,424,957	-				+1.9
4,424,961	-				+0.6
4,424,961	-				+0.5
4,424,989	+				+0.8
4,424,989	+				+0.1
4,424,989	+				+0.5
4,424,989	+				+1.8
4,424,989	+				-0.7
4,424,989	+				-1.5
4,424,989	+				-2.3
4,424,990	-				-0.9
4,424,990	-				-4.3
4,424,990	-				-0.6
4,424,990	-				+1.0
4,424,990	-				+0.5
4,425,006	+				-0.9
4,425,036	+				-1.0
4,425,049	+				-0.3
4,425,049	+				+0.2
4,425,049	+				+1.0
4,425,049	+				-0.2
4,425,049	+				-3.7
4,425,050	-				-3.7
4,425,050	-				+1.1
4,425,053	+				-1.6
4,425,053	+				-0.3
4,425,053	+				+1.3
4,425,053	+				-1.6
4,425,054	-				+0.9
4,425,054	-				+0.5
4,425,188	+				+1.3
4,425,188	+				-0.8
4,425,189	-				-1.2
4,425,189	-				+2.1
4,425,201	+				-3.5
4,425,202	-				-0.4
4,425,222	+				-0.4
4,425,222	+				-0.4
4,425,222	+				+0.1
4,425,222	+				-0.5
4,425,222	+				+0.4
4,425,223	-				+0.8
4,425,223	-				+0.3
4,425,226	+				+0.8
4,425,226	+				-1.4
4,425,226	+				-2.9
4,425,226	+				+1.1
4,425,226	+				+0.7
4,425,226	+				-0.2
4,425,226	+				-0.3
4,425,226	+				+2.1
4,425,226	+				+0.6
4,425,227	-				-0.5
4,425,227	-				-3.9
4,425,227	-				-4.2
4,425,227	-				-0.4
4,425,227	-				-0.2
4,425,227	-				-0.8
4,425,229	-				-0.1
4,425,229	-				+1.3
4,425,229	-				+0.6
4,425,229	-				-3.1
4,425,299	-				-1.0
4,425,306	+				+1.3
4,425,307	-				+0.4
4,425,307	-				+0.5
4,425,373	+				+0.0
4,425,374	-				+1.2
4,425,374	-				+0.9
4,425,406	+				+1.7
4,425,406	+				-0.1
4,425,406	+				-0.2
4,425,406	+				-0.1
4,425,406	+				-3.3
4,425,418	+				+1.1
4,425,418	+				+1.4
4,425,418	+				-0.2
4,425,419	-				-1.2
4,425,419	-				+0.1
4,425,450	+				-2.0
4,425,488	-				-0.7
4,425,489	+				+2.3
4,425,579	+				+1.1
4,425,580	-				+0.8
4,425,723	-				+0.3
4,425,723	-				-1.5
4,425,758	+				+0.7
4,425,758	+				-2.4
4,425,758	+				-2.2
4,425,758	+				-0.1
4,425,759	-				+0.8
4,425,759	-				+1.2
4,425,759	-				+0.7
4,425,759	-				+0.1
4,425,762	+				-0.2
4,425,762	+				-1.2
4,425,763	-				+1.1
4,425,763	-				-3.5
4,425,763	-				+0.5
4,425,763	-				+0.0
4,425,872	+		PP_3917	0.38	+0.8
4,425,873	-		PP_3917	0.39	-0.1
4,425,873	-		PP_3917	0.39	+0.6