Strain Fitness in Pseudomonas putida KT2440 around PP_4686

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
5,324,564	+		PP_4684	0.77	+0.6
5,324,564	+		PP_4684	0.77	+1.5
5,324,565	-		PP_4684	0.77	-2.6
5,324,633	+		PP_4684	0.81	-3.4
5,324,634	-		PP_4684	0.81	-2.3
5,324,927	+				-0.5
5,324,927	+				+0.2
5,324,928	-				-0.4
5,324,928	-				-0.0
5,324,928	-				+0.5
5,324,928	-				+0.8
5,325,049	+				-0.4
5,325,202	+		PP_4685	0.41	-0.0
5,325,203	-		PP_4685	0.41	+1.2
5,325,250	+		PP_4685	0.58	-0.5
5,325,250	+		PP_4685	0.58	+0.1
5,325,250	+		PP_4685	0.58	-0.1
5,325,250	+		PP_4685	0.58	-2.2
5,325,250	+		PP_4685	0.58	+0.9
5,325,251	-		PP_4685	0.58	-1.9
5,325,251	-		PP_4685	0.58	-0.7
5,325,251	-		PP_4685	0.58	-1.2
5,325,251	-		PP_4685	0.58	+0.3
5,325,251	-		PP_4685	0.58	+0.5
5,325,251	-		PP_4685	0.58	+1.0
5,325,294	-		PP_4685	0.74	+0.8
5,325,364	+				+0.1
5,325,443	+				+1.6
5,325,443	+				+0.9
5,325,443	+				+1.3
5,325,443	+				-0.1
5,325,443	+				+0.4
5,325,443	+				-0.5
5,325,444	-				+0.2
5,325,444	-				+1.9
5,325,444	-				+0.8
5,325,444	-				-2.2
5,325,471	+				-0.2
5,325,471	+				-3.9
5,325,471	+				+1.5
5,325,472	-				-1.2
5,325,488	-				+1.0
5,325,488	-				+0.3
5,325,569	+				+1.1
5,325,570	-				+3.6
5,325,570	-				+0.5
5,325,570	-				-1.4
5,325,605	+				+1.1
5,325,605	+				-3.1
5,325,605	+				+1.3
5,325,606	-				-4.0
5,325,606	-				+1.0
5,325,606	-				+0.2
5,325,606	-				+0.4
5,325,606	-				-1.7
5,325,606	-				-2.0
5,325,656	-		PP_4686	0.12	-0.4
5,325,656	-		PP_4686	0.12	+0.4
5,325,700	+		PP_4686	0.17	+1.5
5,325,700	+		PP_4686	0.17	-3.8
5,325,701	-		PP_4686	0.17	+1.0
5,325,725	+		PP_4686	0.20	-2.4
5,325,725	+		PP_4686	0.20	+0.4
5,325,725	+		PP_4686	0.20	+2.1
5,325,725	+		PP_4686	0.20	+0.9
5,325,725	+		PP_4686	0.20	+0.2
5,325,725	+		PP_4686	0.20	-0.2
5,325,726	-		PP_4686	0.20	+0.7
5,325,726	-		PP_4686	0.20	-1.6
5,325,726	-		PP_4686	0.20	+1.5
5,326,016	+		PP_4686	0.53	+0.9
5,326,273	+		PP_4686	0.82	+0.4
5,326,273	+		PP_4686	0.82	-1.1
5,326,273	+		PP_4686	0.82	+1.0
5,326,273	+		PP_4686	0.82	+1.3
5,326,274	-		PP_4686	0.82	+0.7
5,326,274	-		PP_4686	0.82	+1.8
5,326,274	-		PP_4686	0.82	+0.6
5,326,274	-		PP_4686	0.82	+1.6
5,326,274	-		PP_4686	0.82	+1.0
5,326,381	+				+0.8
5,326,390	+				+0.3
5,326,439	+				+1.9
5,326,439	+				+1.6
5,326,439	+				+0.4
5,326,440	-				-2.2
5,326,440	-				-1.5
5,326,440	-				+0.4
5,326,460	+				+0.8
5,326,460	+				-0.2
5,326,461	-				+0.1
5,326,461	-				+1.1
5,326,585	+				-2.0
5,326,585	+				+0.6
5,326,585	+				+0.8
5,326,585	+				+0.1
5,326,598	+				+0.7
5,326,598	+				+0.7
5,326,599	-				+2.3
5,326,599	-				+1.8
5,326,599	-				-1.0
5,326,599	-				-0.1
5,326,680	+		PP_4687	0.19	-1.8
5,326,759	+		PP_4687	0.29	+1.8
5,326,760	-		PP_4687	0.29	+0.7
5,326,760	-		PP_4687	0.29	-1.3
5,326,849	+		PP_4687	0.41	+1.2
5,326,849	+		PP_4687	0.41	-1.4
5,326,850	-		PP_4687	0.41	+0.5
5,326,850	-		PP_4687	0.41	-2.3
5,326,911	-		PP_4687	0.49	-3.1
5,327,279	+				+1.6
5,327,279	+				-0.4
5,327,280	-				-1.5
5,327,280	-				+0.5
5,327,280	-				+0.2
5,327,280	-				-3.6
5,327,280	-				+0.5
5,327,280	-				-2.5
5,327,363	+				-0.6
5,327,363	+				+0.8