Strain Fitness in Pseudomonas putida KT2440 around PP_4256

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
4,840,081	+		PP_4255	0.31	+0.5
4,840,109	+		PP_4255	0.33	+0.1
4,840,109	+		PP_4255	0.33	+0.8
4,840,109	+		PP_4255	0.33	-3.1
4,840,109	+		PP_4255	0.33	+0.7
4,840,109	+		PP_4255	0.33	+0.8
4,840,109	+		PP_4255	0.33	-1.1
4,840,109	+		PP_4255	0.33	-0.3
4,840,109	+		PP_4255	0.33	-1.8
4,840,109	+		PP_4255	0.33	+0.8
4,840,110	-		PP_4255	0.33	-1.7
4,840,110	-		PP_4255	0.33	+2.0
4,840,110	-		PP_4255	0.33	-2.4
4,840,110	-		PP_4255	0.33	-0.1
4,840,110	-		PP_4255	0.33	-1.6
4,840,110	-		PP_4255	0.33	-0.6
4,840,110	-		PP_4255	0.33	-2.1
4,840,110	-		PP_4255	0.33	+1.3
4,840,127	+		PP_4255	0.34	+1.4
4,840,127	+		PP_4255	0.34	-1.0
4,840,304	+		PP_4255	0.46	+0.2
4,840,305	-		PP_4255	0.46	+0.7
4,840,305	-		PP_4255	0.46	-0.1
4,840,307	+		PP_4255	0.47	+0.6
4,840,307	+		PP_4255	0.47	+0.6
4,840,307	+		PP_4255	0.47	+0.8
4,840,308	-		PP_4255	0.47	-3.0
4,840,308	-		PP_4255	0.47	-0.9
4,840,347	-		PP_4255	0.49	-0.4
4,840,347	-		PP_4255	0.49	-2.5
4,840,388	+		PP_4255	0.52	-0.3
4,840,418	+		PP_4255	0.54	-2.1
4,840,418	+		PP_4255	0.54	-1.9
4,840,418	+		PP_4255	0.54	-2.9
4,840,418	+		PP_4255	0.54	-0.3
4,840,458	+		PP_4255	0.57	+0.4
4,840,458	+		PP_4255	0.57	+0.6
4,840,459	-		PP_4255	0.57	-0.0
4,840,459	-		PP_4255	0.57	-0.3
4,840,459	-		PP_4255	0.57	+0.9
4,840,586	+		PP_4255	0.66	+1.1
4,840,587	-		PP_4255	0.66	+1.8
4,840,587	-		PP_4255	0.66	-3.2
4,840,587	-		PP_4255	0.66	+0.1
4,840,649	+		PP_4255	0.70	-0.9
4,840,746	-		PP_4255	0.77	+0.6
4,840,816	-		PP_4255	0.82	-0.9
4,840,816	-		PP_4255	0.82	-3.5
4,840,821	-		PP_4255	0.82	-0.7
4,840,821	-		PP_4255	0.82	-0.3
4,840,821	-		PP_4255	0.82	-2.0
4,840,899	-		PP_4255	0.88	-1.4
4,840,899	-		PP_4255	0.88	-0.7
4,840,899	-		PP_4255	0.88	-1.4
4,840,937	+				+0.2
4,840,994	+				-1.6
4,840,995	-				+0.7
4,840,995	-				-2.3
4,841,014	-				-0.5
4,841,105	+				+0.8
4,841,105	+				+1.2
4,841,105	+				-0.8
4,841,105	+				-1.6
4,841,105	+				-0.9
4,841,106	-				+0.0
4,841,106	-				-0.0
4,841,106	-				-2.2
4,841,106	-				-0.1
4,841,106	-				-0.4
4,841,106	-				-2.1
4,841,248	-		PP_4256	0.28	-3.0
4,841,251	+		PP_4256	0.29	+0.7
4,841,251	+		PP_4256	0.29	+0.0
4,841,251	+		PP_4256	0.29	-0.0
4,841,252	-		PP_4256	0.29	-0.3
4,841,252	-		PP_4256	0.29	-1.5
4,841,252	-		PP_4256	0.29	+0.4
4,841,252	-		PP_4256	0.29	-3.1
4,841,450	-		PP_4256	0.61	-1.2
4,841,573	-		PP_4256	0.81	-2.2
4,841,644	+				+0.2
4,841,645	-				-2.1
4,841,645	-				-2.9
4,841,900	+				-1.6
4,841,900	+				-3.7
4,841,900	+				-2.3
4,841,900	+				-0.6
4,841,901	-				+0.4
4,841,901	-				+0.2
4,841,901	-				+0.2
4,841,901	-				+0.5
4,841,905	+				-2.1
4,842,027	-		PP_4258	0.15	-1.6
4,842,093	-		PP_4258	0.22	-1.9
4,842,149	+		PP_4258	0.28	+1.1
4,842,150	-		PP_4258	0.28	+0.9
4,842,649	+		PP_4258	0.79	+0.9
4,842,649	+		PP_4258	0.79	+2.0