Strain Fitness in Pseudomonas putida KT2440 around PP_3857

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
4,379,542	+				+0.7
4,379,542	+				+0.1
4,379,542	+				-0.3
4,379,543	-				-1.9
4,379,543	-				-1.0
4,379,543	-				-0.9
4,379,572	+				+1.1
4,379,572	+				-0.3
4,379,572	+				+0.3
4,379,573	-				-1.6
4,379,573	-				-2.3
4,379,574	-				-0.9
4,379,580	+				+0.8
4,379,580	+				-0.6
4,379,580	+				+0.9
4,379,580	+				+0.9
4,379,580	+				+0.7
4,379,581	-				-2.2
4,379,581	-				-1.5
4,379,582	+				+0.7
4,379,583	-				+1.0
4,379,631	+				+1.0
4,379,631	+				+0.3
4,379,632	-				-1.1
4,379,632	-				-2.4
4,379,695	-				-1.0
4,379,695	-				+1.0
4,379,740	+				+0.1
4,379,763	-				-0.0
4,379,763	-				-1.4
4,379,766	-				-0.5
4,379,783	+				+0.1
4,379,784	-				+0.7
4,379,784	-				-0.7
4,379,791	+				+2.7
4,379,791	+				-0.5
4,379,866	-		PP_3856	0.14	+0.6
4,379,880	+		PP_3856	0.16	-2.0
4,379,881	-		PP_3856	0.16	+1.5
4,379,881	-		PP_3856	0.16	-0.5
4,379,881	-		PP_3856	0.16	-2.1
4,379,881	-		PP_3856	0.16	+0.8
4,379,968	+		PP_3856	0.27	-3.2
4,379,972	+				-1.0
4,379,973	-		PP_3856	0.28	+1.5
4,379,973	-		PP_3856	0.28	-0.7
4,379,986	-		PP_3856	0.29	-1.8
4,380,002	+		PP_3856	0.32	-0.5
4,380,003	-				-0.3
4,380,053	+		PP_3856	0.38	+0.5
4,380,053	+				+1.3
4,380,054	-		PP_3856	0.38	+0.6
4,380,125	+		PP_3856	0.48	-0.3
4,380,125	+		PP_3856	0.48	-2.7
4,380,125	+		PP_3856	0.48	+0.5
4,380,125	+		PP_3856	0.48	+1.3
4,380,125	+		PP_3856	0.48	+0.6
4,380,126	-		PP_3856	0.48	+2.5
4,380,237	+		PP_3856	0.62	+0.7
4,380,237	+		PP_3856	0.62	-0.2
4,380,237	+		PP_3856	0.62	-1.9
4,380,237	+				-1.3
4,380,238	-		PP_3856	0.62	-0.7
4,380,250	+		PP_3856	0.64	+0.7
4,380,250	+		PP_3856	0.64	+0.4
4,380,250	+		PP_3856	0.64	+1.0
4,380,251	-		PP_3856	0.64	-0.0
4,380,265	-		PP_3856	0.66	+1.2
4,380,345	+		PP_3856	0.76	-1.0
4,380,351	+		PP_3856	0.77	+0.2
4,380,352	-		PP_3856	0.77	+0.4
4,380,517	+				-1.5
4,380,517	+				-1.3
4,380,518	-				+0.3
4,380,518	-				-0.2
4,380,518	-				-0.5
4,380,518	-				-0.9
4,380,551	+				-0.1
4,380,551	+				+1.3
4,380,551	+				+0.6
4,380,552	-				-1.4
4,380,552	-				+1.3
4,380,552	-				-2.4
4,380,552	-				-1.6
4,380,578	+				-1.0
4,380,578	+				+0.4
4,380,579	-				-0.6
4,380,579	-				-1.7
4,380,579	-				+1.8
4,380,579	-				+0.3
4,380,579	-				-2.0
4,380,579	-				+0.6
4,380,667	+				+0.6
4,380,667	+				+0.5
4,380,668	-				-4.4
4,380,668	-				-3.6
4,380,784	+		PP_3857	0.11	-4.1
4,380,785	-		PP_3857	0.11	-0.9
4,380,798	-		PP_3857	0.12	-3.5
4,380,820	-		PP_3857	0.13	-1.1
4,380,863	+		PP_3857	0.14	+1.0
4,380,896	+		PP_3857	0.16	+1.0
4,380,897	-		PP_3857	0.16	+0.7
4,380,900	+		PP_3857	0.16	+0.7
4,380,900	+		PP_3857	0.16	+1.7
4,380,900	+		PP_3857	0.16	-0.5
4,380,900	+		PP_3857	0.16	-1.3
4,380,900	+		PP_3857	0.16	-1.2
4,380,901	-		PP_3857	0.16	+1.1
4,380,901	-		PP_3857	0.16	-1.3
4,380,901	-		PP_3857	0.16	-0.7
4,380,942	+		PP_3857	0.18	-0.3
4,380,943	-		PP_3857	0.18	+2.2
4,380,970	+		PP_3857	0.19	+0.0
4,380,988	+		PP_3857	0.20	+0.0
4,380,988	+		PP_3857	0.20	-2.5
4,381,021	+		PP_3857	0.21	-3.5
4,381,022	-		PP_3857	0.22	-2.4
4,381,027	+		PP_3857	0.22	-2.6
4,381,033	+		PP_3857	0.22	+0.9
4,381,034	-		PP_3857	0.22	-1.9
4,381,189	+		PP_3857	0.29	-4.3
4,381,189	+		PP_3857	0.29	+0.6
4,381,190	-		PP_3857	0.29	+0.1
4,381,288	+		PP_3857	0.33	+0.5
4,381,288	+		PP_3857	0.33	+0.8
4,381,288	+		PP_3857	0.33	-1.5
4,381,288	+		PP_3857	0.33	-0.5
4,381,289	-		PP_3857	0.33	+0.2
4,381,289	-		PP_3857	0.33	-2.6
4,381,334	+		PP_3857	0.35	-0.2
4,381,334	+		PP_3857	0.35	+0.2
4,381,417	+		PP_3857	0.39	+1.2
4,381,418	-		PP_3857	0.39	-0.6
4,381,418	-		PP_3857	0.39	+0.3
4,381,418	-		PP_3857	0.39	-1.4
4,381,422	+		PP_3857	0.39	+1.3
4,381,422	+		PP_3857	0.39	-0.7
4,381,423	-		PP_3857	0.39	+0.7
4,381,423	-		PP_3857	0.39	+1.2
4,381,423	-		PP_3857	0.39	-0.3
4,381,537	+				-2.0
4,381,537	+		PP_3857	0.44	-3.5
4,381,537	+		PP_3857	0.44	-2.0
4,381,538	-		PP_3857	0.44	+1.1
4,381,538	-		PP_3857	0.44	-1.1
4,381,538	-		PP_3857	0.44	+1.0
4,381,570	+		PP_3857	0.46	+0.1
4,381,570	+				-0.3
4,381,571	-		PP_3857	0.46	+0.9
4,381,571	-		PP_3857	0.46	-0.8
4,381,571	-		PP_3857	0.46	+0.2
4,381,571	-		PP_3857	0.46	-1.2
4,381,571	-		PP_3857	0.46	+4.1
4,381,588	+		PP_3857	0.47	+0.7
4,381,617	-		PP_3857	0.48	+0.5
4,381,617	-		PP_3857	0.48	+0.8
4,381,641	+		PP_3857	0.49	-3.7
4,381,641	+		PP_3857	0.49	+0.2
4,381,641	+		PP_3857	0.49	+0.5
4,381,641	+		PP_3857	0.49	+0.9
4,381,641	+		PP_3857	0.49	+0.9
4,381,641	+		PP_3857	0.49	-1.9
4,381,642	-		PP_3857	0.49	-2.7
4,381,642	-		PP_3857	0.49	+1.0
4,381,642	-		PP_3857	0.49	-1.1
4,381,694	+		PP_3857	0.51	-0.2
4,381,694	+		PP_3857	0.51	+1.3
4,381,694	+		PP_3857	0.51	+1.2
4,381,695	-		PP_3857	0.51	+0.0
4,381,695	-		PP_3857	0.51	+0.2
4,381,823	+		PP_3857	0.57	-1.1
4,381,847	+		PP_3857	0.58	+1.2
4,381,877	+		PP_3857	0.59	+0.8
4,381,914	+		PP_3857	0.61	+0.1
4,381,915	-		PP_3857	0.61	-0.0
4,382,027	+		PP_3857	0.66	-2.9
4,382,027	+		PP_3857	0.66	-0.5
4,382,073	+		PP_3857	0.68	+2.1
4,382,073	+		PP_3857	0.68	-0.3
4,382,073	+		PP_3857	0.68	-1.6
4,382,073	+		PP_3857	0.68	+0.1
4,382,073	+		PP_3857	0.68	-0.3
4,382,097	+		PP_3857	0.69	-0.3
4,382,097	+		PP_3857	0.69	+0.1
4,382,098	-		PP_3857	0.69	-1.5
4,382,098	-		PP_3857	0.69	-0.6
4,382,098	-		PP_3857	0.69	-0.7
4,382,100	+		PP_3857	0.69	-3.1
4,382,100	+		PP_3857	0.69	-2.3
4,382,172	+		PP_3857	0.73	-2.6
4,382,172	+		PP_3857	0.73	-1.2
4,382,172	+		PP_3857	0.73	-1.9
4,382,173	-		PP_3857	0.73	+0.7
4,382,173	-		PP_3857	0.73	-2.8
4,382,173	-		PP_3857	0.73	-0.3
4,382,173	-		PP_3857	0.73	+0.2
4,382,240	+		PP_3857	0.76	+1.2
4,382,240	+		PP_3857	0.76	+0.0
4,382,240	+		PP_3857	0.76	-2.4
4,382,241	-		PP_3857	0.76	+0.4
4,382,307	-		PP_3857	0.79	-2.2
4,382,364	-		PP_3857	0.81	-0.4
4,382,367	-		PP_3857	0.81	-0.7
4,382,462	-		PP_3857	0.85	+1.1
4,382,462	-		PP_3857	0.85	-5.2
4,382,462	-		PP_3857	0.85	-0.5
4,382,462	-		PP_3857	0.85	-0.7
4,382,462	-		PP_3857	0.85	-2.1
4,382,547	+		PP_3857	0.89	+1.0
4,382,547	+		PP_3857	0.89	-1.3
4,382,547	+		PP_3857	0.89	+0.4
4,382,548	-		PP_3857	0.89	-1.5
4,382,548	-		PP_3857	0.89	-0.1
4,382,557	+		PP_3857	0.90	-1.0
4,382,557	+		PP_3857	0.90	+1.8
4,382,557	+		PP_3857	0.90	-2.3
4,382,558	-		PP_3857	0.90	-0.5
4,382,582	+				-0.9
4,382,612	+				+0.8
4,382,620	+				+0.4
4,382,621	-				+1.6
4,382,640	+				-1.8
4,382,641	-				-0.7
4,382,698	+				-0.9
4,382,698	+				-0.6
4,382,698	+				+0.7
4,382,698	+				-1.1
4,382,698	+				-0.4
4,382,698	+				+0.5
4,382,762	-				-0.3
4,383,077	-				-3.5
4,383,093	-				-1.5
4,383,149	+		PP_3858	0.12	-1.3
4,383,149	+		PP_3858	0.12	-1.7
4,383,149	+		PP_3858	0.12	-0.3
4,383,150	-		PP_3858	0.12	-0.1
4,383,150	-		PP_3858	0.12	-1.4
4,383,193	+		PP_3858	0.18	-3.2
4,383,193	+		PP_3858	0.18	+0.5
4,383,193	+		PP_3858	0.18	+1.0
4,383,193	+		PP_3858	0.18	-0.8
4,383,194	-		PP_3858	0.18	+0.4
4,383,207	-		PP_3858	0.19	+1.2
4,383,271	+		PP_3858	0.27	+0.7
4,383,271	+		PP_3858	0.27	-0.1
4,383,271	+		PP_3858	0.27	-3.4
4,383,272	-		PP_3858	0.27	+0.5
4,383,272	-		PP_3858	0.27	-0.6
4,383,384	-		PP_3858	0.41	+0.6
4,383,385	+		PP_3858	0.42	+1.5
4,383,385	+		PP_3858	0.42	-2.3
4,383,385	+		PP_3858	0.42	-0.3
4,383,386	-		PP_3858	0.42	+0.1
4,383,386	-		PP_3858	0.42	+1.0
4,383,386	-		PP_3858	0.42	+0.7
4,383,386	-		PP_3858	0.42	-3.5
4,383,386	-		PP_3858	0.42	-2.8
4,383,404	-		PP_3858	0.44	-1.0
4,383,404	-		PP_3858	0.44	+0.4
4,383,421	+		PP_3858	0.46	+1.0
4,383,421	+		PP_3858	0.46	-1.2
4,383,421	+		PP_3858	0.46	-1.3
4,383,421	+		PP_3858	0.46	-0.3
4,383,421	+		PP_3858	0.46	-3.2
4,383,421	+		PP_3858	0.46	-1.5
4,383,421	+		PP_3858	0.46	+0.5
4,383,422	-		PP_3858	0.46	-0.5
4,383,422	-		PP_3858	0.46	+0.3
4,383,422	-		PP_3858	0.46	+0.2
4,383,440	-		PP_3858	0.48	-1.7
4,383,505	+		PP_3858	0.56	+0.6
4,383,506	-		PP_3858	0.57	+1.4
4,383,523	+		PP_3858	0.59	+1.1
4,383,568	+		PP_3858	0.64	-3.4
4,383,569	-		PP_3858	0.64	-2.5
4,383,589	+		PP_3858	0.67	+0.2
4,383,590	-		PP_3858	0.67	+0.7
4,383,706	+		PP_3858	0.81	+0.5
4,383,717	-		PP_3858	0.83	-0.6
4,383,744	-		PP_3858	0.86	+0.4
4,383,770	+		PP_3858	0.89	+0.3
4,383,771	-		PP_3858	0.90	+1.3