Strain Fitness in Pseudomonas putida KT2440 around PP_3548

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
4,022,014	-		PP_3547	0.69	-0.4
4,022,014	-		PP_3547	0.69	+0.5
4,022,148	+		PP_3547	0.83	-0.7
4,022,148	+		PP_3547	0.83	-1.9
4,022,148	+		PP_3547	0.83	-2.2
4,022,148	+		PP_3547	0.83	-1.2
4,022,602	+				-1.3
4,022,602	+				-0.7
4,022,602	+				+0.8
4,022,876	+				+1.3
4,022,887	+				-2.6
4,023,009	-				-2.2
4,023,077	-				+0.0
4,023,077	-				+0.6
4,023,097	+				-1.3
4,023,097	+				+1.0
4,023,097	+				+0.5
4,023,097	+				+0.4
4,023,098	-				+0.6
4,023,098	-				-3.1
4,023,098	-				-0.5
4,023,098	-				-0.2
4,023,098	-				+0.4
4,023,115	-				-1.3
4,023,134	-		PP_3548	0.10	+1.2
4,023,134	-		PP_3548	0.10	-0.0
4,023,134	-		PP_3548	0.10	+0.3
4,023,170	+		PP_3548	0.13	+0.3
4,023,171	-		PP_3548	0.13	-1.2
4,023,214	+		PP_3548	0.16	-2.3
4,023,215	-		PP_3548	0.16	+0.4
4,023,215	-		PP_3548	0.16	+0.7
4,023,215	-		PP_3548	0.16	+0.0
4,023,457	+		PP_3548	0.32	-0.5
4,023,457	+		PP_3548	0.32	+0.4
4,023,457	+		PP_3548	0.32	-1.6
4,023,457	+		PP_3548	0.32	+0.9
4,023,457	+		PP_3548	0.32	-0.3
4,023,457	+		PP_3548	0.32	+2.3
4,023,457	+		PP_3548	0.32	-0.9
4,023,457	+		PP_3548	0.32	-2.8
4,023,457	+		PP_3548	0.32	-0.7
4,023,458	-		PP_3548	0.32	-0.2
4,023,523	+		PP_3548	0.36	-2.3
4,023,523	+		PP_3548	0.36	-1.0
4,023,599	+		PP_3548	0.41	+1.9
4,023,599	+		PP_3548	0.41	-1.2
4,023,601	+		PP_3548	0.41	-0.1
4,023,601	+		PP_3548	0.41	+0.3
4,023,601	+		PP_3548	0.41	-0.8
4,023,850	-				-1.9
4,023,850	-		PP_3548	0.57	-0.8
4,023,895	+		PP_3548	0.60	-4.5
4,023,895	+		PP_3548	0.60	+0.5
4,023,895	+		PP_3548	0.60	-1.8
4,023,895	+		PP_3548	0.60	-1.2
4,023,895	+				-0.0
4,023,895	+		PP_3548	0.60	-1.4
4,023,896	-		PP_3548	0.60	-0.2
4,023,896	-		PP_3548	0.60	+0.0
4,023,896	-		PP_3548	0.60	-1.3
4,023,896	-		PP_3548	0.60	-0.6
4,023,896	-		PP_3548	0.60	+1.2
4,024,009	+		PP_3548	0.67	-0.2
4,024,009	+		PP_3548	0.67	-1.1
4,024,009	+		PP_3548	0.67	-0.7
4,024,009	+		PP_3548	0.67	-0.1
4,024,010	-		PP_3548	0.68	-0.9
4,024,010	-		PP_3548	0.68	+0.4
4,024,010	-		PP_3548	0.68	+2.0
4,024,010	-		PP_3548	0.68	-1.2
4,024,013	+		PP_3548	0.68	-0.7
4,024,014	-		PP_3548	0.68	+0.5
4,024,145	-		PP_3548	0.76	+1.7
4,024,360	+				-0.3
4,024,360	+				+1.7
4,024,360	+				-1.3
4,024,361	-				-1.1
4,024,361	-				+0.1
4,024,361	-				+0.5
4,024,590	+				-0.4
4,024,590	+				-1.2
4,024,590	+				-0.2
4,024,591	-				+0.3
4,024,605	+				+0.7
4,024,606	-				+1.7
4,024,606	-				-0.6
4,024,846	-		PP_3549	0.29	-1.9
4,024,863	+		PP_3549	0.30	-0.3
4,024,864	-		PP_3549	0.30	+1.7
4,025,012	-		PP_3549	0.42	+1.0
4,025,012	-		PP_3549	0.42	+0.9
4,025,026	-		PP_3549	0.44	-4.4
4,025,435	+		PP_3549	0.78	-0.5
4,025,435	+		PP_3549	0.78	+1.0
4,025,435	+		PP_3549	0.78	+0.8
4,025,483	-		PP_3549	0.82	+0.5