Strain Fitness in Pseudomonas putida KT2440 around PP_2242

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
2,556,762	+		PP_2241	0.54	+1.8
2,556,762	+		PP_2241	0.54	-1.0
2,556,807	+		PP_2241	0.58	-3.5
2,556,808	-		PP_2241	0.58	+1.2
2,556,840	+		PP_2241	0.61	-1.8
2,556,841	-		PP_2241	0.61	-0.7
2,557,003	-		PP_2241	0.74	-1.1
2,557,003	-		PP_2241	0.74	-3.8
2,557,059	-		PP_2241	0.79	+2.0
2,557,174	+		PP_2241	0.89	-0.7
2,557,174	+		PP_2241	0.89	-1.1
2,557,174	+		PP_2241	0.89	+0.6
2,557,175	-		PP_2241	0.89	-1.1
2,557,175	-		PP_2241	0.89	-0.3
2,557,175	-		PP_2241	0.89	-3.0
2,557,175	-		PP_2241	0.89	-1.2
2,557,175	-		PP_2241	0.89	+0.3
2,557,331	+				-0.8
2,557,344	+				-0.6
2,557,344	+				-2.0
2,557,345	-				-1.6
2,557,345	-				-0.5
2,557,382	+				+2.5
2,557,383	-				-3.0
2,557,419	+				+1.5
2,557,420	-				+0.6
2,557,435	+				+1.5
2,557,436	-				+1.2
2,557,483	+				+1.8
2,557,503	+				+0.7
2,557,590	-				+1.5
2,557,632	-				-0.4
2,557,736	+				+0.3
2,557,736	+				+0.0
2,557,737	-				-0.1
2,558,145	-		PP_2242	0.18	-1.0
2,558,149	+		PP_2242	0.18	-1.6
2,558,173	-		PP_2242	0.19	-2.5
2,558,332	+		PP_2242	0.26	-0.2
2,558,498	+		PP_2242	0.34	-1.6
2,558,671	+		PP_2242	0.42	-1.5
2,558,672	-		PP_2242	0.42	-1.0
2,558,672	-		PP_2242	0.42	-2.4
2,558,703	+		PP_2242	0.43	+1.2
2,558,703	+		PP_2242	0.43	-0.3
2,558,704	-		PP_2242	0.43	-3.5
2,558,704	-		PP_2242	0.43	+0.8
2,558,707	+		PP_2242	0.43	-0.3
2,558,707	+		PP_2242	0.43	-4.4
2,558,707	+		PP_2242	0.43	+1.3
2,558,707	+		PP_2242	0.43	-0.1
2,558,707	+		PP_2242	0.43	+0.5
2,558,708	-		PP_2242	0.43	-0.3
2,558,708	-		PP_2242	0.43	-0.9
2,558,743	-		PP_2242	0.45	-1.8
2,558,770	+		PP_2242	0.46	+0.8
2,558,770	+		PP_2242	0.46	-1.0
2,558,770	+		PP_2242	0.46	-1.1
2,558,771	-		PP_2242	0.46	+1.2
2,558,775	-				-0.8
2,558,775	-		PP_2242	0.46	+0.9
2,558,775	-		PP_2242	0.46	-1.4
2,558,855	+		PP_2242	0.50	-1.7
2,558,855	+		PP_2242	0.50	-0.2
2,558,922	+		PP_2242	0.53	-0.3
2,558,922	+		PP_2242	0.53	-4.8
2,558,922	+		PP_2242	0.53	-1.2
2,558,922	+		PP_2242	0.53	+0.9
2,558,923	-		PP_2242	0.53	-0.5
2,558,923	-		PP_2242	0.53	-0.3
2,558,923	-		PP_2242	0.53	-0.3
2,558,938	+		PP_2242	0.54	+0.6
2,558,938	+		PP_2242	0.54	+0.1
2,558,938	+		PP_2242	0.54	+0.7
2,558,944	+		PP_2242	0.54	-1.8
2,559,055	+		PP_2242	0.59	-1.7
2,559,056	-		PP_2242	0.59	-3.7
2,559,056	-		PP_2242	0.59	+0.1
2,559,198	-		PP_2242	0.65	+0.8
2,559,220	+		PP_2242	0.66	+0.1
2,559,238	+		PP_2242	0.67	-0.6
2,559,238	+		PP_2242	0.67	+1.9
2,559,256	+		PP_2242	0.68	-2.3
2,559,256	+		PP_2242	0.68	-0.9
2,559,257	-		PP_2242	0.68	-0.8
2,559,257	-		PP_2242	0.68	+0.2
2,559,266	-		PP_2242	0.68	+2.4
2,559,266	-		PP_2242	0.68	+2.6
2,559,266	-		PP_2242	0.68	-3.0
2,559,316	+		PP_2242	0.70	-3.7
2,559,317	-		PP_2242	0.71	-0.1
2,559,379	+		PP_2242	0.73	-0.7
2,559,412	+		PP_2242	0.75	-0.0
2,559,428	-		PP_2242	0.75	-0.9
2,559,463	+		PP_2242	0.77	-1.6
2,559,463	+		PP_2242	0.77	+0.6
2,559,463	+		PP_2242	0.77	-0.0
2,559,464	-		PP_2242	0.77	-0.0
2,559,464	-		PP_2242	0.77	-3.4
2,559,712	+		PP_2242	0.88	+0.2
2,559,712	+		PP_2242	0.88	+0.6
2,559,712	+		PP_2242	0.88	+0.5
2,559,713	-		PP_2242	0.88	-1.8
2,559,713	-		PP_2242	0.88	-0.4
2,559,713	-		PP_2242	0.88	-4.7
2,559,713	-		PP_2242	0.88	+0.1
2,559,713	-		PP_2242	0.88	+0.3
2,559,739	+		PP_2242	0.89	-0.3
2,559,764	-				+0.6
2,559,764	-				-0.3
2,559,764	-				+0.9
2,559,797	-				-1.6
2,559,841	+				-1.2
2,559,841	+				+1.2
2,559,842	-				+0.3
2,559,842	-				-2.2
2,559,842	-				+2.0
2,559,925	+				-1.8
2,559,925	+				+0.4
2,559,925	+				-0.3
2,559,926	-				-1.3
2,559,926	-				+0.6
2,559,926	-				+0.2
2,559,926	-				-1.7
2,559,926	-				+0.4
2,559,926	-				-0.1
2,559,928	-				-0.6
2,559,928	-				-0.2
2,559,963	+				-0.3
2,559,963	+				-0.4
2,559,963	+				-0.2
2,560,065	+				-0.3
2,560,066	-				+1.2
2,560,358	+		PP_2243	0.53	-1.8
2,560,358	+		PP_2243	0.53	-2.0
2,560,358	+		PP_2243	0.53	+0.3
2,560,359	-		PP_2243	0.53	+1.2
2,560,359	-		PP_2243	0.53	+0.2
2,560,443	-		PP_2243	0.67	-1.2
2,560,535	+		PP_2243	0.82	-3.1
2,560,536	-		PP_2243	0.82	-0.2
2,560,536	-		PP_2243	0.82	-1.5
2,560,611	+				+2.2
2,560,639	+				-2.9
2,560,742	+		PP_2244	0.12	-0.4
2,560,742	+		PP_2244	0.12	+1.8
2,560,743	-		PP_2244	0.12	-3.7
2,560,743	-		PP_2244	0.12	-2.8
2,560,892	+		PP_2244	0.31	+0.9
2,560,955	-		PP_2244	0.39	-0.4
2,560,955	-		PP_2244	0.39	-1.2