Strain Fitness in Pseudomonas putida KT2440 around PP_2178

Experiment: 3-methyl-1-butanol 0.25 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.25 vol% (C)
2,482,209	-		PP_2176	0.82	-0.3
2,482,209	-		PP_2176	0.82	+0.8
2,482,298	+				-1.5
2,482,298	+				+0.7
2,482,298	+				+0.6
2,482,299	-				-1.6
2,482,521	+				+1.4
2,482,592	-				-1.7
2,482,752	-		PP_2177	0.36	-1.6
2,482,758	-		PP_2177	0.37	+0.8
2,482,758	-		PP_2177	0.37	+1.0
2,482,758	-		PP_2177	0.37	+1.0
2,482,758	-		PP_2177	0.37	+0.4
2,482,821	-		PP_2177	0.48	-0.9
2,482,925	+		PP_2177	0.67	-1.2
2,482,926	-		PP_2177	0.67	+0.0
2,482,926	-		PP_2177	0.67	-0.1
2,483,058	-				-0.9
2,483,128	+				-0.7
2,483,129	-				-0.2
2,483,205	-				+0.2
2,483,243	+				+0.6
2,483,579	+		PP_2178	0.33	+0.1
2,483,579	+		PP_2178	0.33	-1.7
2,483,580	-		PP_2178	0.33	+0.5
2,483,580	-		PP_2178	0.33	+0.7
2,483,618	+		PP_2178	0.36	-0.3
2,483,619	-		PP_2178	0.36	-0.9
2,483,857	+		PP_2178	0.54	+1.5
2,483,903	+		PP_2178	0.57	-0.8
2,483,904	-		PP_2178	0.57	+0.7
2,483,904	-		PP_2178	0.57	+1.9
2,483,949	-		PP_2178	0.60	+0.5
2,483,965	+		PP_2178	0.62	-3.2
2,483,966	-		PP_2178	0.62	+1.2
2,484,072	-		PP_2178	0.70	-2.1
2,484,215	+		PP_2178	0.80	-4.5
2,484,216	-		PP_2178	0.80	-1.0
2,484,216	-		PP_2178	0.80	+0.7
2,484,216	-		PP_2178	0.80	+1.0
2,484,404	+				+1.7
2,484,404	+				+0.2
2,484,404	+				-3.2
2,484,405	-				+0.6
2,484,420	-				-0.2
2,484,420	-				-2.3
2,484,480	-				+0.7
2,484,521	+				+0.2
2,484,532	+				-1.9
2,484,565	+				+1.4
2,484,565	+				-0.1
2,484,565	+				+0.2
2,484,565	+				+0.9
2,484,565	+				-3.1
2,484,566	-				-0.6
2,484,648	-		PP_2179	0.11	-3.2
2,484,791	+		PP_2179	0.29	-3.7
2,484,791	+		PP_2179	0.29	-1.5
2,484,792	-		PP_2179	0.29	-2.3
2,484,792	-		PP_2179	0.29	+0.6
2,484,792	-		PP_2179	0.29	+0.5
2,484,886	-		PP_2179	0.41	-2.0
2,484,891	+		PP_2179	0.42	-0.2
2,485,044	+		PP_2179	0.62	+0.9
2,485,044	+		PP_2179	0.62	-0.3
2,485,044	+		PP_2179	0.62	-0.5
2,485,045	-		PP_2179	0.62	+1.2
2,485,126	-		PP_2179	0.72	+0.7
2,485,259	+		PP_2179	0.90	-0.3
2,485,308	+				-1.0
2,485,360	+				+1.7