Experiment: 3-methyl-1-butanol 0.25 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1215 and PP_1216 are separated by 109 nucleotides PP_1216 and PP_1217 overlap by 4 nucleotides PP_1217 and PP_1218 are separated by 125 nucleotides PP_1218 and PP_1219 are separated by 2 nucleotides
PP_1215: PP_1215 - Crossover junction endodeoxyribonuclease RuvC, at 1,393,016 to 1,393,540
_1215
PP_1216: PP_1216 - Holliday junction ATP-dependent DNA helicase RuvA, at 1,393,650 to 1,394,267
_1216
PP_1217: PP_1217 - Holliday junction ATP-dependent DNA helicase RuvB, at 1,394,264 to 1,395,310
_1217
PP_1218: PP_1218 - putative acyl-CoA thioesterase, at 1,395,436 to 1,395,888
_1218
PP_1219: PP_1219 - subunit of a proton-driven complex used for maintenance of outer membrane stability, at 1,395,891 to 1,396,586
_1219
Position (kb)
1394
1395
1396 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1393.538 kb on + strand at 1393.558 kb on - strand at 1393.577 kb on + strand at 1393.577 kb on + strand at 1393.577 kb on + strand at 1393.577 kb on + strand at 1393.578 kb on - strand at 1393.602 kb on + strand at 1393.602 kb on + strand at 1393.703 kb on + strand at 1393.704 kb on - strand at 1393.756 kb on - strand, within PP_1216 at 1393.794 kb on - strand, within PP_1216 at 1394.099 kb on - strand, within PP_1216 at 1394.160 kb on + strand, within PP_1216 at 1394.160 kb on + strand, within PP_1216 at 1394.161 kb on - strand, within PP_1216 at 1394.354 kb on + strand at 1394.354 kb on + strand at 1394.355 kb on - strand at 1394.355 kb on - strand at 1394.355 kb on - strand at 1394.705 kb on - strand, within PP_1217 at 1394.744 kb on - strand, within PP_1217 at 1394.747 kb on - strand, within PP_1217 at 1394.814 kb on - strand, within PP_1217 at 1394.891 kb on + strand, within PP_1217 at 1394.961 kb on - strand, within PP_1217 at 1395.148 kb on + strand, within PP_1217 at 1395.148 kb on + strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.149 kb on - strand, within PP_1217 at 1395.173 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.191 kb on + strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.192 kb on - strand, within PP_1217 at 1395.236 kb on + strand at 1395.236 kb on + strand at 1395.237 kb on - strand at 1395.237 kb on - strand at 1395.237 kb on - strand at 1395.256 kb on + strand at 1395.256 kb on + strand at 1395.257 kb on - strand at 1395.257 kb on - strand at 1395.257 kb on - strand at 1395.377 kb on + strand at 1395.377 kb on + strand at 1395.423 kb on + strand at 1395.423 kb on + strand at 1395.430 kb on + strand at 1395.430 kb on + strand at 1395.495 kb on + strand, within PP_1218 at 1395.517 kb on + strand, within PP_1218 at 1395.517 kb on + strand, within PP_1218 at 1395.520 kb on + strand, within PP_1218 at 1395.520 kb on + strand, within PP_1218 at 1395.725 kb on + strand, within PP_1218 at 1395.821 kb on + strand, within PP_1218
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.25 vol% (C) remove 1,393,538 + -4.4 1,393,558 - -0.2 1,393,577 + -3.4 1,393,577 + +0.9 1,393,577 + +1.6 1,393,577 + -2.8 1,393,578 - -2.4 1,393,602 + +2.3 1,393,602 + +0.3 1,393,703 + -1.9 1,393,704 - -3.4 1,393,756 - PP_1216 0.17 +0.5 1,393,794 - PP_1216 0.23 +1.8 1,394,099 - PP_1216 0.73 -2.5 1,394,160 + PP_1216 0.83 -2.9 1,394,160 + PP_1216 0.83 -2.8 1,394,161 - PP_1216 0.83 -2.3 1,394,354 + -0.1 1,394,354 + -1.6 1,394,355 - -1.5 1,394,355 - -2.3 1,394,355 - -2.4 1,394,705 - PP_1217 0.42 -2.6 1,394,744 - PP_1217 0.46 -1.5 1,394,747 - PP_1217 0.46 -2.3 1,394,814 - PP_1217 0.53 -2.1 1,394,891 + PP_1217 0.60 +0.3 1,394,961 - PP_1217 0.67 -1.8 1,395,148 + PP_1217 0.84 -1.8 1,395,148 + PP_1217 0.84 -0.1 1,395,149 - PP_1217 0.85 -0.8 1,395,149 - PP_1217 0.85 +1.5 1,395,149 - PP_1217 0.85 -1.6 1,395,149 - PP_1217 0.85 -0.5 1,395,149 - PP_1217 0.85 -1.8 1,395,173 + PP_1217 0.87 -2.5 1,395,191 + PP_1217 0.89 -2.1 1,395,191 + PP_1217 0.89 +0.5 1,395,191 + PP_1217 0.89 -1.8 1,395,192 - PP_1217 0.89 -1.1 1,395,192 - PP_1217 0.89 -1.1 1,395,192 - PP_1217 0.89 -1.1 1,395,192 - PP_1217 0.89 +1.5 1,395,236 + -0.8 1,395,236 + -0.5 1,395,237 - -1.8 1,395,237 - -0.1 1,395,237 - +0.6 1,395,256 + +1.0 1,395,256 + +2.1 1,395,257 - -1.7 1,395,257 - -2.9 1,395,257 - -3.4 1,395,377 + -2.9 1,395,377 + -0.7 1,395,423 + -0.7 1,395,423 + +0.9 1,395,430 + +0.9 1,395,430 + +2.2 1,395,495 + PP_1218 0.13 +1.2 1,395,517 + PP_1218 0.18 -2.3 1,395,517 + PP_1218 0.18 -0.6 1,395,520 + PP_1218 0.19 +2.8 1,395,520 + PP_1218 0.19 -0.5 1,395,725 + PP_1218 0.64 -0.6 1,395,821 + PP_1218 0.85 -1.9
Or see this region's nucleotide sequence