Experiment: 3-methyl-1-butanol 0.25 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0722 and PP_t69 are separated by 48 nucleotides PP_t69 and PP_0723 are separated by 20 nucleotides PP_0723 and PP_0724 are separated by 4 nucleotides PP_0724 and PP_0725 are separated by 4 nucleotides
PP_0722: PP_0722 - Ribose-phosphate pyrophosphokinase, at 838,756 to 839,697
_0722
PP_t69: PP_t69 - tRNA-Gln, at 839,746 to 839,820
_t69
PP_0723: PP_0723 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 839,841 to 840,701
_0723
PP_0724: PP_0724 - Outer-membrane lipoprotein LolB, at 840,706 to 841,323
_0724
PP_0725: PP_0725 - conserved hypothetical TPR repeat protein, at 841,328 to 843,049
_0725
Position (kb) 839
840
841 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 839.722 kb on - strand at 839.722 kb on - strand at 839.724 kb on - strand at 839.724 kb on - strand at 839.736 kb on - strand at 839.738 kb on - strand at 839.877 kb on - strand at 839.877 kb on - strand at 841.376 kb on - strand at 841.413 kb on - strand at 841.513 kb on - strand, within PP_0725 at 841.631 kb on - strand, within PP_0725 at 841.633 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.25 vol% (C) remove 839,722 - +0.4 839,722 - +0.9 839,724 - -0.8 839,724 - -1.1 839,736 - -2.2 839,738 - -2.1 839,877 - +1.3 839,877 - +0.2 841,376 - -3.7 841,413 - +2.1 841,513 - PP_0725 0.11 -3.4 841,631 - PP_0725 0.18 -1.5 841,633 - PP_0725 0.18 -1.2 841,660 - PP_0725 0.19 -3.8 841,660 - PP_0725 0.19 -1.5
Or see this region's nucleotide sequence