Experiment: 3-methyl-1-butanol 0.25 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5449 and PP_0639 are separated by 199 nucleotides PP_0639 and PP_0640 overlap by 4 nucleotides PP_0640 and PP_5450 are separated by 143 nucleotides
PP_5449: PP_5449 - conserved exported protein of unknown function, at 747,948 to 748,766
_5449
PP_0639: PP_0639 - conserved protein of unknown function, at 748,966 to 749,682
_0639
PP_0640: PP_0640 - conserved protein of unknown function, at 749,679 to 750,863
_0640
PP_5450: PP_5450 - exported protein of unknown function, at 751,007 to 751,606
_5450
Position (kb) 749
750
751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 748.738 kb on - strand at 748.773 kb on - strand at 748.812 kb on + strand at 748.812 kb on + strand at 748.879 kb on + strand at 748.880 kb on - strand at 749.101 kb on - strand, within PP_0639 at 749.141 kb on + strand, within PP_0639 at 749.239 kb on - strand, within PP_0639 at 749.279 kb on - strand, within PP_0639 at 749.308 kb on + strand, within PP_0639 at 749.309 kb on - strand, within PP_0639 at 749.309 kb on - strand, within PP_0639 at 749.315 kb on - strand, within PP_0639 at 749.355 kb on + strand, within PP_0639 at 749.421 kb on - strand, within PP_0639 at 749.652 kb on - strand at 749.652 kb on - strand at 749.704 kb on + strand at 750.454 kb on - strand, within PP_0640 at 750.492 kb on - strand, within PP_0640 at 750.593 kb on + strand, within PP_0640 at 750.616 kb on + strand, within PP_0640 at 750.617 kb on - strand, within PP_0640 at 750.658 kb on - strand, within PP_0640 at 750.841 kb on + strand at 750.915 kb on + strand at 750.916 kb on - strand at 750.916 kb on - strand at 750.927 kb on + strand at 750.933 kb on - strand at 750.937 kb on + strand at 750.938 kb on - strand at 750.938 kb on - strand at 750.938 kb on - strand at 750.950 kb on + strand at 750.950 kb on + strand at 750.950 kb on + strand at 750.951 kb on - strand at 750.994 kb on + strand at 750.994 kb on + strand at 751.004 kb on + strand at 751.008 kb on + strand at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.135 kb on - strand, within PP_5450 at 751.145 kb on + strand, within PP_5450 at 751.145 kb on + strand, within PP_5450 at 751.277 kb on - strand, within PP_5450 at 751.284 kb on - strand, within PP_5450 at 751.372 kb on + strand, within PP_5450 at 751.372 kb on + strand, within PP_5450 at 751.373 kb on - strand, within PP_5450 at 751.373 kb on - strand, within PP_5450 at 751.373 kb on - strand at 751.392 kb on - strand, within PP_5450 at 751.402 kb on + strand, within PP_5450 at 751.403 kb on - strand, within PP_5450 at 751.476 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.489 kb on - strand, within PP_5450 at 751.513 kb on + strand, within PP_5450 at 751.555 kb on + strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.580 kb on + strand at 751.609 kb on - strand at 751.609 kb on - strand at 751.621 kb on + strand at 751.622 kb on - strand at 751.647 kb on + strand at 751.647 kb on + strand at 751.648 kb on - strand at 751.648 kb on - strand at 751.752 kb on - strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.771 kb on - strand at 751.771 kb on - strand at 751.797 kb on - strand at 751.829 kb on + strand at 751.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.25 vol% (C) remove 748,738 - -0.9 748,773 - -2.4 748,812 + -0.1 748,812 + +0.9 748,879 + -0.0 748,880 - -3.1 749,101 - PP_0639 0.19 -0.2 749,141 + PP_0639 0.24 -3.6 749,239 - PP_0639 0.38 -4.7 749,279 - PP_0639 0.44 +0.2 749,308 + PP_0639 0.48 +0.7 749,309 - PP_0639 0.48 -0.5 749,309 - PP_0639 0.48 +0.0 749,315 - PP_0639 0.49 +0.3 749,355 + PP_0639 0.54 -0.3 749,421 - PP_0639 0.63 -0.2 749,652 - -2.9 749,652 - -0.4 749,704 + -2.5 750,454 - PP_0640 0.65 -3.5 750,492 - PP_0640 0.69 -3.0 750,593 + PP_0640 0.77 -0.2 750,616 + PP_0640 0.79 -1.1 750,617 - PP_0640 0.79 -2.2 750,658 - PP_0640 0.83 -3.3 750,841 + +0.3 750,915 + +1.0 750,916 - +1.6 750,916 - -0.3 750,927 + +2.2 750,933 - -0.3 750,937 + +1.0 750,938 - -2.9 750,938 - -3.4 750,938 - -3.3 750,950 + +2.6 750,950 + -2.8 750,950 + +1.9 750,951 - -0.5 750,994 + -1.4 750,994 + +0.9 751,004 + -2.7 751,008 + -1.8 751,134 + PP_5450 0.21 -0.2 751,134 + PP_5450 0.21 +0.1 751,134 + PP_5450 0.21 +0.9 751,134 + PP_5450 0.21 +1.0 751,135 - PP_5450 0.21 +2.9 751,145 + PP_5450 0.23 -1.7 751,145 + PP_5450 0.23 +0.3 751,277 - PP_5450 0.45 +0.3 751,284 - PP_5450 0.46 +0.7 751,372 + PP_5450 0.61 +2.7 751,372 + PP_5450 0.61 -0.0 751,373 - PP_5450 0.61 +0.3 751,373 - PP_5450 0.61 +0.3 751,373 - +0.3 751,392 - PP_5450 0.64 +3.5 751,402 + PP_5450 0.66 -0.9 751,403 - PP_5450 0.66 +2.2 751,476 - PP_5450 0.78 +0.6 751,480 - PP_5450 0.79 -3.8 751,480 - PP_5450 0.79 +1.5 751,480 - PP_5450 0.79 +1.6 751,489 - PP_5450 0.80 +0.6 751,513 + PP_5450 0.84 +0.4 751,555 + -1.9 751,556 - +1.6 751,556 - -0.6 751,556 - -1.7 751,580 + -0.8 751,609 - +0.7 751,609 - +0.1 751,621 + -1.6 751,622 - -0.4 751,647 + +0.7 751,647 + +0.8 751,648 - +0.5 751,648 - +2.0 751,752 - -2.5 751,770 + -1.2 751,770 + +0.4 751,771 - +0.2 751,771 - +0.1 751,797 - -0.1 751,829 + +1.3 751,830 - -0.4
Or see this region's nucleotide sequence