Strain Fitness in Pseudomonas putida KT2440 around PP_5300

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
6,046,758	+		PP_5299	0.46	+1.4
6,046,798	-		PP_5299	0.49	-0.3
6,046,936	-		PP_5299	0.59	-1.5
6,047,047	-		PP_5299	0.67	-2.4
6,047,075	-		PP_5299	0.69	-0.6
6,047,299	+		PP_5299	0.86	+0.2
6,047,299	+		PP_5299	0.86	-0.4
6,047,344	-		PP_5299	0.89	+0.6
6,047,344	-		PP_5299	0.89	+0.1
6,047,344	-		PP_5299	0.89	+1.0
6,047,384	+				-0.4
6,047,385	-				-0.9
6,047,385	-				-1.6
6,047,417	+				+0.0
6,047,418	-				-0.4
6,047,418	-				-0.2
6,047,421	+				+2.0
6,047,422	-				-0.0
6,047,422	-				+0.9
6,047,422	-				+0.4
6,047,422	-				+0.4
6,047,502	+				+1.5
6,047,502	+				-0.9
6,047,502	+				-0.4
6,047,502	+				-1.5
6,047,502	+				+0.0
6,047,503	-				+0.2
6,047,503	-				+1.1
6,047,503	-				-1.3
6,047,503	-				+0.3
6,047,503	-				+0.8
6,047,536	-				+0.0
6,047,648	+		PP_5300	0.12	+0.2
6,047,648	+		PP_5300	0.12	+0.4
6,047,649	-		PP_5300	0.12	-2.3
6,047,710	-		PP_5300	0.18	-1.3
6,047,878	-		PP_5300	0.34	-1.3
6,047,999	+		PP_5300	0.45	-0.2
6,048,064	-		PP_5300	0.51	-0.6
6,048,198	+		PP_5300	0.64	-0.5
6,048,198	+		PP_5300	0.64	-0.7
6,048,198	+		PP_5300	0.64	-0.1
6,048,198	+		PP_5300	0.64	+0.9
6,048,198	+		PP_5300	0.64	+1.5
6,048,199	-		PP_5300	0.64	-0.4
6,048,199	-		PP_5300	0.64	-0.1
6,048,199	-		PP_5300	0.64	-3.3
6,048,202	+		PP_5300	0.64	-0.6
6,048,202	+		PP_5300	0.64	-0.6
6,048,202	+		PP_5300	0.64	-1.2
6,048,202	+		PP_5300	0.64	-1.6
6,048,202	+		PP_5300	0.64	+0.5
6,048,202	+		PP_5300	0.64	+0.1
6,048,203	-		PP_5300	0.64	+0.9
6,048,203	-		PP_5300	0.64	-0.5
6,048,203	-		PP_5300	0.64	+0.4
6,048,203	-		PP_5300	0.64	-1.1
6,048,203	-		PP_5300	0.64	-2.0
6,048,296	+		PP_5300	0.73	+0.8
6,048,343	-		PP_5300	0.78	+1.6
6,048,380	-		PP_5300	0.81	+0.9
6,048,415	-		PP_5300	0.85	-0.1
6,048,415	-		PP_5300	0.85	+0.4
6,048,415	-		PP_5300	0.85	-1.5
6,048,415	-		PP_5300	0.85	+0.9
6,048,415	-		PP_5300	0.85	-1.0
6,048,580	-				-1.5
6,048,640	+				+0.2
6,048,647	+				-1.5
6,048,774	+				+0.3
6,048,802	+				-0.5