Strain Fitness in Pseudomonas putida KT2440 around PP_4724

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
5,371,281	+		PP_4723	0.80	-1.8
5,371,282	-		PP_4723	0.80	-0.5
5,371,380	+		PP_4723	0.83	-2.1
5,371,381	-		PP_4723	0.83	-2.2
5,371,381	-		PP_4723	0.83	-1.2
5,371,734	+				-1.8
5,371,735	-				-3.3
5,371,845	+				-2.8
5,371,915	+				-2.6
5,372,267	-		PP_4724	0.21	-3.1
5,372,401	-		PP_4724	0.32	-1.5
5,372,421	+		PP_4724	0.34	+1.8
5,372,422	-		PP_4724	0.34	-1.7
5,372,487	-		PP_4724	0.40	-3.2
5,372,574	+		PP_4724	0.48	-2.2
5,372,574	+		PP_4724	0.48	+0.2
5,372,596	-		PP_4724	0.50	-2.4
5,372,596	-		PP_4724	0.50	-1.2
5,372,667	+		PP_4724	0.56	-0.2
5,372,809	+		PP_4724	0.68	-1.4
5,372,891	-		PP_4724	0.75	-2.1
5,372,977	+		PP_4724	0.83	-0.2
5,373,001	-		PP_4724	0.85	-1.9
5,373,065	-				+0.2
5,373,065	-				-2.0
5,373,065	-				-2.9
5,373,077	+				-1.4
5,373,078	-				-3.3
5,373,078	-				-2.0
5,373,078	-				-3.9
5,373,130	+				-1.0
5,373,131	-				-2.0
5,373,183	-				-2.3
5,373,232	+				-1.4
5,373,233	-				-1.9
5,373,233	-				-2.0
5,373,233	-				-1.3
5,373,233	-				-0.3
5,373,297	+				-3.4
5,373,298	-				-3.5
5,373,306	-				-2.9
5,373,306	-				-2.5
5,373,490	+				+0.8
5,373,491	-				+0.4