Experiment: 3-methyl-1-butanol 0.1 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5706 and PP_4715 are separated by 4 nucleotides PP_4715 and PP_4716 are separated by 65 nucleotides PP_4716 and PP_4717 are separated by 16 nucleotides PP_4717 and PP_4718 are separated by 8 nucleotides
PP_5706: PP_5706 - Sec translocation complex - SecG subunit, at 5,361,201 to 5,361,599
_5706
PP_4715: PP_4715 - triose phosphate isomerase, at 5,361,604 to 5,362,359
_4715
PP_4716: PP_4716 - Phosphoglucosamine mutase, at 5,362,425 to 5,363,765
_4716
PP_4717: PP_4717 - dihydropteroate synthase, at 5,363,782 to 5,364,633
_4717
PP_4718: PP_4718 - integral membrane ATP-dependent zinc metallopeptidase, at 5,364,642 to 5,366,555
_4718
Position (kb)
5362
5363
5364 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5361.426 kb on - strand, within PP_5706 at 5361.442 kb on - strand, within PP_5706 at 5361.522 kb on - strand, within PP_5706 at 5361.547 kb on - strand, within PP_5706 at 5363.766 kb on - strand at 5363.784 kb on - strand at 5364.571 kb on - strand at 5364.634 kb on - strand at 5364.634 kb on - strand at 5364.634 kb on - strand at 5364.644 kb on - strand at 5364.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C) remove 5,361,426 - PP_5706 0.56 +2.9 5,361,442 - PP_5706 0.60 -0.8 5,361,522 - PP_5706 0.80 +0.4 5,361,547 - PP_5706 0.87 -1.0 5,363,766 - -0.8 5,363,784 - -1.6 5,364,571 - -1.5 5,364,634 - -0.6 5,364,634 - -0.3 5,364,634 - +1.8 5,364,644 - -1.5 5,364,644 - -0.1
Or see this region's nucleotide sequence