Strain Fitness in Pseudomonas putida KT2440 around PP_2991

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
3,387,676	+		PP_2989	0.57	-1.8
3,387,676	+		PP_2989	0.57	-0.3
3,387,677	-		PP_2989	0.57	+0.1
3,387,677	-		PP_2989	0.57	+0.2
3,387,748	+		PP_2989	0.66	-0.6
3,387,749	-		PP_2989	0.67	-0.5
3,387,890	+		PP_2989	0.84	-0.1
3,388,053	+				+1.2
3,388,053	+				-1.9
3,388,151	+				+0.5
3,388,151	+				-0.2
3,388,151	+				-1.2
3,388,152	-				+1.9
3,388,152	-				-0.2
3,388,160	-				+0.3
3,388,160	-				+1.5
3,388,160	-				-0.8
3,388,209	+		PP_2990	0.12	+1.1
3,388,209	+		PP_2990	0.12	-1.3
3,388,266	+		PP_2990	0.24	-0.8
3,388,267	-		PP_2990	0.24	+0.4
3,388,298	+		PP_2990	0.30	-0.0
3,388,298	+				-1.4
3,388,298	+		PP_2990	0.30	+1.0
3,388,299	-		PP_2990	0.31	+1.8
3,388,299	-		PP_2990	0.31	+0.8
3,388,299	-		PP_2990	0.31	+0.4
3,388,420	+		PP_2990	0.56	-0.9
3,388,491	-		PP_2990	0.71	+0.5
3,388,535	-		PP_2990	0.80	-3.1
3,388,687	+				+0.9
3,388,688	-				-0.5
3,388,714	+				+0.0
3,388,714	+				-0.0
3,388,715	-				-1.0
3,388,720	+				-2.6
3,388,721	-				-3.6
3,388,757	-		PP_2991	0.11	-1.4
3,388,849	+		PP_2991	0.20	+0.0
3,388,850	-		PP_2991	0.20	-2.0
3,388,880	+		PP_2991	0.23	+0.6
3,388,981	+		PP_2991	0.32	-0.9
3,388,982	-		PP_2991	0.32	+1.9
3,389,030	+		PP_2991	0.37	+0.5
3,389,030	+		PP_2991	0.37	+0.3
3,389,042	-		PP_2991	0.38	+1.6
3,389,094	+		PP_2991	0.43	+0.7
3,389,094	+		PP_2991	0.43	+0.7
3,389,094	+		PP_2991	0.43	+0.5
3,389,095	-		PP_2991	0.43	-1.2
3,389,095	-		PP_2991	0.43	-1.0
3,389,095	-		PP_2991	0.43	-1.3
3,389,095	-		PP_2991	0.43	-0.5
3,389,098	+		PP_2991	0.44	+1.0
3,389,098	+		PP_2991	0.44	-1.1
3,389,098	+		PP_2991	0.44	+0.2
3,389,098	+		PP_2991	0.44	+0.6
3,389,099	-		PP_2991	0.44	+1.0
3,389,099	-		PP_2991	0.44	+0.5
3,389,099	-		PP_2991	0.44	-1.1
3,389,175	-		PP_2991	0.51	+0.2
3,389,224	+		PP_2991	0.56	+0.4
3,389,224	+		PP_2991	0.56	-1.3
3,389,224	+		PP_2991	0.56	+0.9
3,389,224	+		PP_2991	0.56	+1.7
3,389,225	-		PP_2991	0.56	+0.0
3,389,225	-		PP_2991	0.56	+0.8
3,389,225	-		PP_2991	0.56	-0.3
3,389,225	-		PP_2991	0.56	+1.2
3,389,225	-		PP_2991	0.56	-0.9
3,389,225	-		PP_2991	0.56	+1.2
3,389,230	+		PP_2991	0.56	+0.2
3,389,230	+		PP_2991	0.56	+0.8
3,389,230	+		PP_2991	0.56	+0.9
3,389,230	+		PP_2991	0.56	+0.1
3,389,231	-		PP_2991	0.57	+0.1
3,389,231	-		PP_2991	0.57	+0.2
3,389,231	-		PP_2991	0.57	+1.0
3,389,231	-		PP_2991	0.57	-0.3
3,389,237	+		PP_2991	0.57	-0.6
3,389,238	-		PP_2991	0.57	+0.2
3,389,289	+		PP_2991	0.62	+1.9
3,389,290	-		PP_2991	0.62	+0.1
3,389,292	+		PP_2991	0.62	-0.4
3,389,292	+		PP_2991	0.62	-2.9
3,389,369	-		PP_2991	0.70	+0.5
3,389,383	+		PP_2991	0.71	-1.9
3,389,628	+				-0.2
3,389,697	+				+0.3
3,389,698	-				+1.7
3,389,698	-				-0.5
3,389,701	-				+1.4
3,389,736	+				+1.9
3,390,019	+		PP_2992	0.41	+0.4
3,390,019	+		PP_2992	0.41	-0.3
3,390,019	+		PP_2992	0.41	-0.2
3,390,020	-		PP_2992	0.41	+0.0
3,390,054	-		PP_2992	0.46	-0.5
3,390,094	+		PP_2992	0.50	-0.1
3,390,094	+		PP_2992	0.50	-2.2
3,390,095	-		PP_2992	0.51	+0.1
3,390,123	-		PP_2992	0.54	+0.4
3,390,202	+		PP_2992	0.64	-3.3
3,390,202	+		PP_2992	0.64	-1.1
3,390,202	+		PP_2992	0.64	-3.3
3,390,202	+				+0.2
3,390,203	-		PP_2992	0.64	-0.5
3,390,272	-		PP_2992	0.73	-1.7
3,390,272	-		PP_2992	0.73	+0.3
3,390,316	-		PP_2992	0.78	+0.4
3,390,349	+		PP_2992	0.82	+1.7
3,390,368	+		PP_2992	0.85	+0.6
3,390,369	-		PP_2992	0.85	+0.6
3,390,450	+				+0.6
3,390,450	+				-0.6
3,390,450	+				+0.6
3,390,450	+				-0.1
3,390,451	-				-1.8
3,390,451	-				+0.9
3,390,457	-				-0.2
3,390,457	-				-1.4
3,390,532	+				+0.8
3,390,569	-				+1.3
3,390,569	-				+0.0
3,390,569	-				-1.4