Strain Fitness in Pseudomonas putida KT2440 around PP_2818

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
3,213,312	+		PP_2817	0.33	-1.5
3,213,312	+		PP_2817	0.33	-0.4
3,213,313	-		PP_2817	0.33	-0.6
3,213,445	-		PP_2817	0.45	-0.2
3,213,564	+		PP_2817	0.55	-0.5
3,213,792	+		PP_2817	0.75	+0.0
3,213,792	+		PP_2817	0.75	-1.5
3,213,792	+		PP_2817	0.75	-0.9
3,213,792	+		PP_2817	0.75	+0.5
3,213,792	+		PP_2817	0.75	-0.8
3,213,792	+		PP_2817	0.75	-1.8
3,213,792	+		PP_2817	0.75	-1.1
3,213,792	+		PP_2817	0.75	+0.3
3,213,793	-		PP_2817	0.76	-2.0
3,213,793	-		PP_2817	0.76	+1.0
3,213,793	-				+1.7
3,213,793	-		PP_2817	0.76	-1.7
3,213,793	-		PP_2817	0.76	-0.1
3,214,025	+				+0.5
3,214,230	+				+0.2
3,214,314	+				+0.1
3,214,314	+				-1.5
3,214,315	-				-1.2
3,214,504	-		PP_2818	0.13	+0.3
3,214,504	-		PP_2818	0.13	+0.1
3,214,539	-		PP_2818	0.14	+0.8
3,214,549	+		PP_2818	0.15	-0.4
3,214,557	+		PP_2818	0.15	-1.2
3,214,557	+		PP_2818	0.15	+1.0
3,214,558	-		PP_2818	0.15	-2.0
3,214,558	-		PP_2818	0.15	-0.8
3,214,558	-		PP_2818	0.15	-0.3
3,214,674	+		PP_2818	0.19	+0.8
3,214,729	+		PP_2818	0.21	+0.9
3,214,911	+		PP_2818	0.26	+0.6
3,214,912	-		PP_2818	0.26	+0.6
3,214,912	-		PP_2818	0.26	-0.9
3,214,912	-		PP_2818	0.26	-0.3
3,215,056	-		PP_2818	0.31	-2.6
3,215,067	+		PP_2818	0.31	+0.8
3,215,179	-		PP_2818	0.35	+1.5
3,215,179	-		PP_2818	0.35	-0.5
3,215,181	-		PP_2818	0.35	-0.3
3,215,488	-		PP_2818	0.45	-1.6
3,215,662	-		PP_2818	0.50	+0.0
3,215,662	-		PP_2818	0.50	-0.2
3,215,715	+		PP_2818	0.52	-1.2
3,215,715	+		PP_2818	0.52	-2.0
3,215,715	+		PP_2818	0.52	-0.8
3,215,715	+				+2.1
3,215,716	-		PP_2818	0.52	+1.0
3,215,742	+		PP_2818	0.53	+0.1
3,215,742	+		PP_2818	0.53	-1.5
3,215,742	+		PP_2818	0.53	+0.5
3,215,742	+		PP_2818	0.53	-2.2
3,215,748	+		PP_2818	0.53	-0.4
3,215,793	+		PP_2818	0.55	+0.1
3,215,794	-		PP_2818	0.55	+0.9
3,215,794	-		PP_2818	0.55	-0.5
3,215,794	-		PP_2818	0.55	-0.2
3,215,794	-		PP_2818	0.55	-1.0
3,215,796	-		PP_2818	0.55	-1.5
3,216,214	-		PP_2818	0.68	-0.6
3,216,438	+		PP_2818	0.75	-1.4
3,216,438	+		PP_2818	0.75	+0.4
3,216,439	-		PP_2818	0.75	+0.1
3,216,439	-		PP_2818	0.75	-0.1
3,216,526	-		PP_2818	0.78	+0.9
3,217,240	+				+1.3
3,217,241	-				-0.7
3,217,286	+				+0.2
3,217,287	-				+1.1
3,217,287	-				+1.0
3,217,339	+				-0.0
3,217,592	+		PP_2819	0.27	+1.4
3,217,676	+		PP_2819	0.33	+0.4
3,217,677	-		PP_2819	0.33	-3.4
3,217,677	-		PP_2819	0.33	+0.7
3,217,677	-		PP_2819	0.33	-2.4
3,217,941	-		PP_2819	0.52	+1.5
3,218,149	+		PP_2819	0.67	+0.8
3,218,149	+		PP_2819	0.67	+0.3
3,218,150	-		PP_2819	0.67	-1.9
3,218,150	-		PP_2819	0.67	-1.3