Strain Fitness in Pseudomonas putida KT2440 around PP_2299

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_t40 and PP_2298 are separated by 149 nucleotidesPP_2298 and PP_2299 are separated by 230 nucleotidesPP_2299 and PP_2300 are separated by 91 nucleotidesPP_2300 and PP_2301 are separated by 63 nucleotides PP_t40: PP_t40 - tRNA-His, at 2,626,122 to 2,626,197 _t40 PP_2298: PP_2298 - conserved protein of unknown function, at 2,626,347 to 2,626,685 _2298 PP_2299: PP_2299 - Trigger factor, at 2,626,916 to 2,628,247 _2299 PP_2300: PP_2300 - ATP-dependent Clp protease proteolytic subunit, at 2,628,339 to 2,628,980 _2300 PP_2301: PP_2301 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,629,044 to 2,630,372 _2301 Position (kb) 2626 2627 2628 2629Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2625.951 kb on + strandat 2625.951 kb on + strandat 2625.951 kb on + strandat 2625.951 kb on + strandat 2625.951 kb on + strandat 2625.952 kb on - strandat 2625.952 kb on - strandat 2625.952 kb on - strandat 2625.991 kb on + strandat 2625.992 kb on - strandat 2626.057 kb on + strandat 2626.094 kb on + strandat 2626.094 kb on + strandat 2626.095 kb on - strandat 2626.102 kb on + strandat 2626.119 kb on - strandat 2626.142 kb on - strand, within PP_t40at 2626.198 kb on + strandat 2626.302 kb on - strandat 2626.302 kb on - strandat 2626.302 kb on - strandat 2626.536 kb on - strand, within PP_2298at 2626.653 kb on + strandat 2626.707 kb on + strandat 2626.724 kb on - strandat 2626.866 kb on + strandat 2626.867 kb on - strandat 2626.930 kb on - strandat 2627.075 kb on - strand, within PP_2299at 2627.080 kb on - strand, within PP_2299at 2627.208 kb on + strand, within PP_2299at 2627.209 kb on - strand, within PP_2299at 2627.541 kb on + strand, within PP_2299at 2627.541 kb on + strand, within PP_2299at 2627.664 kb on + strand, within PP_2299at 2627.664 kb on + strand, within PP_2299at 2627.665 kb on - strand, within PP_2299at 2628.214 kb on + strandat 2628.302 kb on + strandat 2628.302 kb on + strandat 2628.317 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C)
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2,625,951 + -0.5
2,625,951 + +0.3
2,625,951 + -0.4
2,625,951 + +1.4
2,625,951 + +1.2
2,625,952 - +1.1
2,625,952 - +1.2
2,625,952 - +1.1
2,625,991 + -0.6
2,625,992 - -0.0
2,626,057 + -1.9
2,626,094 + -1.7
2,626,094 + +0.5
2,626,095 - +3.1
2,626,102 + +0.6
2,626,119 - +0.4
2,626,142 - PP_t40 0.26 +0.8
2,626,198 + +0.5
2,626,302 - +2.0
2,626,302 - +1.3
2,626,302 - +1.6
2,626,536 - PP_2298 0.56 +2.3
2,626,653 + -0.8
2,626,707 + -1.9
2,626,724 - +2.0
2,626,866 + +0.4
2,626,867 - +0.5
2,626,930 - +0.4
2,627,075 - PP_2299 0.12 -1.9
2,627,080 - PP_2299 0.12 -0.8
2,627,208 + PP_2299 0.22 +1.8
2,627,209 - PP_2299 0.22 -2.5
2,627,541 + PP_2299 0.47 +1.1
2,627,541 + PP_2299 0.47 -2.1
2,627,664 + PP_2299 0.56 -1.7
2,627,664 + PP_2299 0.56 -3.4
2,627,665 - PP_2299 0.56 -1.3
2,628,214 + -0.5
2,628,302 + +1.7
2,628,302 + -0.1
2,628,317 + +1.4

Or see this region's nucleotide sequence