Strain Fitness in Pseudomonas putida KT2440 around PP_2200

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
2,505,542	+		PP_2199	0.13	+0.6
2,505,542	+		PP_2199	0.13	+0.7
2,505,543	-		PP_2199	0.13	+0.0
2,505,543	-		PP_2199	0.13	-2.3
2,505,801	+		PP_2199	0.36	-0.4
2,505,801	+		PP_2199	0.36	+0.5
2,505,802	-		PP_2199	0.36	+1.3
2,505,802	-		PP_2199	0.36	+0.3
2,505,927	+		PP_2199	0.48	-2.8
2,505,928	-		PP_2199	0.48	-0.2
2,506,078	+		PP_2199	0.61	-0.2
2,506,212	+		PP_2199	0.73	-1.5
2,506,256	-		PP_2199	0.77	-1.0
2,506,373	+		PP_2199	0.88	-0.2
2,506,374	-		PP_2199	0.88	+2.3
2,506,374	-		PP_2199	0.88	-1.0
2,506,394	+		PP_2199	0.90	+0.5
2,506,394	+		PP_2199	0.90	+1.4
2,506,428	-				+0.1
2,506,440	-				+0.3
2,506,455	-				+1.2
2,506,540	-				-0.1
2,506,540	-				+0.9
2,506,557	+				-0.2
2,506,558	-				+0.7
2,506,558	-				-0.6
2,506,559	+				-2.7
2,506,559	+				-0.1
2,506,587	+				+1.4
2,506,695	+		PP_2200	0.30	+0.7
2,506,695	+		PP_2200	0.30	-0.9
2,506,696	-		PP_2200	0.30	+0.0
2,506,696	-		PP_2200	0.30	-0.0
2,506,703	+		PP_2200	0.31	-0.8
2,506,703	+		PP_2200	0.31	-0.9
2,506,704	-		PP_2200	0.31	+0.1
2,506,704	-		PP_2200	0.31	-1.6
2,506,704	-		PP_2200	0.31	-1.5
2,506,704	-		PP_2200	0.31	+0.3
2,506,837	-		PP_2200	0.54	-1.2
2,506,873	-		PP_2200	0.60	+1.0
2,506,873	-		PP_2200	0.60	-0.1
2,506,988	+		PP_2200	0.80	-2.4
2,507,100	+				+0.7
2,507,101	-				+0.7
2,507,138	-				-0.2
2,507,147	+				-0.7
2,507,147	+				+1.8
2,507,148	-				+0.2
2,507,311	+		PP_2201	0.14	+0.1
2,507,311	+		PP_2201	0.14	+0.1
2,507,311	+		PP_2201	0.14	-0.4
2,507,311	+		PP_2201	0.14	-2.1
2,507,311	+		PP_2201	0.14	-0.3
2,507,312	-				-2.0
2,507,312	-		PP_2201	0.14	-0.2
2,507,331	+		PP_2201	0.16	-0.4
2,507,332	-		PP_2201	0.16	-0.2
2,507,332	-		PP_2201	0.16	+0.4
2,507,426	-		PP_2201	0.25	-0.8
2,507,453	-		PP_2201	0.28	-1.3
2,507,453	-		PP_2201	0.28	+0.0
2,507,561	-		PP_2201	0.39	-0.4
2,507,575	+		PP_2201	0.40	+0.8
2,507,576	-		PP_2201	0.40	-1.4
2,507,576	-		PP_2201	0.40	+0.3
2,507,583	+		PP_2201	0.41	+0.4
2,507,584	-				+2.4
2,507,617	+		PP_2201	0.44	-1.2
2,507,618	-		PP_2201	0.44	+0.8
2,507,713	+		PP_2201	0.54	+0.4
2,507,714	-		PP_2201	0.54	-0.0
2,507,833	+		PP_2201	0.66	+1.1
2,508,013	+		PP_2201	0.84	-0.2
2,508,100	+				+1.4
2,508,100	+				-1.1
2,508,100	+				+1.1