Strain Fitness in Pseudomonas putida KT2440 around PP_2017

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
2,286,801	-		PP_2015	0.70	+2.4
2,286,870	+				+0.8
2,286,870	+				-0.8
2,286,871	-				+4.0
2,286,915	-				-1.4
2,286,940	+				-1.2
2,287,010	-		PP_2016	0.16	-0.1
2,287,072	+		PP_2016	0.24	-0.6
2,287,072	+		PP_2016	0.24	+0.1
2,287,141	+		PP_2016	0.32	-0.4
2,287,208	-		PP_2016	0.40	+0.8
2,287,633	+				-1.1
2,287,633	+				-2.5
2,287,672	+				+0.3
2,287,672	+				-0.1
2,287,673	-				-1.0
2,287,673	-				-3.4
2,287,673	-				+0.0
2,287,681	+				+0.6
2,287,682	-				-1.1
2,287,745	+				-1.1
2,287,746	-				-0.3
2,287,757	+				-1.8
2,287,758	-				-0.4
2,287,772	+				-0.1
2,287,955	+				-1.2
2,287,955	+				+0.6
2,287,956	-				-0.3
2,287,956	-				-1.1
2,288,063	+		PP_2017	0.13	-2.7
2,288,063	+		PP_2017	0.13	-2.3
2,288,123	+		PP_2017	0.15	+1.7
2,288,123	+		PP_2017	0.15	-1.0
2,288,123	+		PP_2017	0.15	-1.2
2,288,124	-		PP_2017	0.15	+0.2
2,288,124	-		PP_2017	0.15	+0.3
2,288,124	-		PP_2017	0.15	-1.4
2,288,124	-		PP_2017	0.15	-3.5
2,288,124	-		PP_2017	0.15	+0.1
2,288,126	+		PP_2017	0.15	-0.4
2,288,127	-		PP_2017	0.15	+0.3
2,288,127	-		PP_2017	0.15	+0.5
2,288,288	+		PP_2017	0.21	-2.2
2,288,375	+		PP_2017	0.25	-1.0
2,288,376	-		PP_2017	0.25	-2.0
2,288,376	-		PP_2017	0.25	+1.0
2,288,456	+		PP_2017	0.28	-0.7
2,288,468	+		PP_2017	0.28	+0.4
2,288,707	-		PP_2017	0.37	-1.4
2,288,775	-		PP_2017	0.40	-1.9
2,288,862	-		PP_2017	0.43	-2.9
2,288,939	-		PP_2017	0.46	-0.4
2,288,939	-		PP_2017	0.46	+0.4
2,288,954	+		PP_2017	0.47	-0.5
2,288,955	-		PP_2017	0.47	-0.5
2,288,955	-		PP_2017	0.47	-0.3
2,289,053	+				+0.4
2,289,053	+		PP_2017	0.50	-0.4
2,289,054	-		PP_2017	0.50	-0.0
2,289,095	+		PP_2017	0.52	-2.5
2,289,096	-		PP_2017	0.52	+0.4
2,289,096	-		PP_2017	0.52	-0.1
2,289,472	-		PP_2017	0.66	-2.3
2,289,564	+		PP_2017	0.69	+0.2
2,289,565	-		PP_2017	0.69	-1.9
2,289,565	-		PP_2017	0.69	-0.2
2,289,622	+		PP_2017	0.72	+0.6
2,289,622	+		PP_2017	0.72	+0.1
2,289,726	-		PP_2017	0.76	-1.6
2,289,761	+		PP_2017	0.77	+0.4
2,289,761	+		PP_2017	0.77	-2.7
2,289,762	-		PP_2017	0.77	-2.8
2,289,762	-		PP_2017	0.77	-1.4
2,289,818	+		PP_2017	0.79	-0.2
2,289,847	-		PP_2017	0.80	-1.8
2,289,847	-		PP_2017	0.80	-0.4
2,289,915	+		PP_2017	0.83	+0.6
2,290,268	+				+1.4
2,290,268	+				-2.2
2,290,268	+				-1.0
2,290,347	-				-0.2
2,290,347	-				-0.4
2,290,347	-				-3.2
2,290,347	-				-0.1
2,290,373	+				-0.1
2,290,433	+				-1.2
2,290,433	+				-1.3
2,290,434	-				+1.1
2,290,434	-				+0.4
2,290,434	-				+0.6
2,290,451	-				+1.8
2,290,471	+				-3.4
2,290,487	+				+0.8
2,290,487	+				-0.7
2,290,488	-				+1.9
2,290,501	-				+0.4
2,290,501	-				+3.3
2,290,582	+				+0.2
2,290,582	+				+1.0
2,290,582	+				+1.8
2,290,583	-				+0.4
2,290,586	+				+1.0
2,290,586	+				+1.1
2,290,587	-				+0.3
2,290,587	-				+1.2
2,290,587	-				+1.8
2,290,587	-				-1.4
2,290,689	+				+1.1
2,290,690	-				-2.1
2,290,808	+		PP_2018	0.16	+0.7
2,291,025	+		PP_2018	0.39	+0.1
2,291,025	+		PP_2018	0.39	+0.4
2,291,025	+		PP_2018	0.39	+0.6
2,291,025	+		PP_2018	0.39	+1.3
2,291,026	-		PP_2018	0.39	+0.9
2,291,026	-		PP_2018	0.39	+1.3