Strain Fitness in Pseudomonas putida KT2440 around PP_1075

Experiment: 3-methyl-1-butanol 0.1 vol% (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 0.1 vol% (C)
1,232,974	-				+1.5
1,233,322	-		PP_1074	0.52	+1.5
1,233,322	-		PP_1074	0.52	+0.2
1,233,431	-		PP_1074	0.66	+2.1
1,233,485	+		PP_1074	0.74	+1.3
1,233,512	+		PP_1074	0.77	-0.4
1,233,513	-		PP_1074	0.77	+0.3
1,233,629	+				+0.9
1,233,630	-				+1.5
1,233,630	-				-0.1
1,233,730	-				-0.2
1,233,750	+				+0.1
1,233,784	+				-0.6
1,233,784	+				-3.0
1,233,784	+				-1.1
1,233,784	+				-3.4
1,233,785	-				-1.4
1,233,785	-				-2.7
1,233,785	-				-4.0
1,233,785	-				-2.3
1,233,785	-				-1.8
1,233,844	+				-2.2
1,233,844	+				-1.7
1,233,844	+				-2.1
1,233,844	+				-2.9
1,233,958	+		PP_1075	0.11	-4.1
1,233,959	-		PP_1075	0.12	-3.0
1,234,136	-		PP_1075	0.23	-2.1
1,234,173	+		PP_1075	0.26	-1.1
1,234,174	-		PP_1075	0.26	-2.0
1,234,174	-		PP_1075	0.26	-2.6
1,234,216	+		PP_1075	0.29	-0.4
1,234,216	+		PP_1075	0.29	-2.5
1,234,265	-		PP_1075	0.32	-3.0
1,234,359	-		PP_1075	0.38	-2.3
1,234,359	-		PP_1075	0.38	-0.6
1,234,359	-		PP_1075	0.38	-2.7
1,234,393	+		PP_1075	0.40	-1.0
1,234,394	-		PP_1075	0.41	-2.6
1,234,394	-		PP_1075	0.41	-2.0
1,234,394	-		PP_1075	0.41	+0.0
1,234,498	+		PP_1075	0.47	-2.1
1,234,499	-		PP_1075	0.48	-0.9
1,234,499	-		PP_1075	0.48	-2.2
1,234,499	-		PP_1075	0.48	-1.2
1,234,502	+		PP_1075	0.48	-2.3
1,234,502	+		PP_1075	0.48	-0.7
1,234,502	+		PP_1075	0.48	-2.7
1,234,502	+		PP_1075	0.48	-3.7
1,234,503	-		PP_1075	0.48	-2.0
1,234,503	-		PP_1075	0.48	-1.7
1,234,503	-		PP_1075	0.48	+0.4
1,234,503	-		PP_1075	0.48	-1.3
1,234,503	-		PP_1075	0.48	-3.1
1,234,503	-		PP_1075	0.48	-3.5
1,234,503	-		PP_1075	0.48	+0.3
1,234,581	+		PP_1075	0.53	-1.5
1,234,581	+		PP_1075	0.53	-0.6
1,234,582	-		PP_1075	0.53	-2.3
1,234,604	-		PP_1075	0.55	-2.5
1,234,754	+		PP_1075	0.65	-2.7
1,234,755	-		PP_1075	0.65	-4.0
1,234,871	-		PP_1075	0.72	-3.7
1,234,906	+		PP_1075	0.75	-1.9
1,234,906	+		PP_1075	0.75	-2.1
1,234,907	-		PP_1075	0.75	-0.5
1,234,982	-		PP_1075	0.80	-2.9
1,235,158	+				-2.5
1,235,159	-				-3.5
1,235,186	+				-1.3
1,235,186	+				-2.0
1,235,186	+				-3.0
1,235,187	-				-1.6
1,235,190	+				-2.9
1,235,190	+				-0.4
1,235,190	+				-1.6
1,235,190	+				-1.7
1,235,191	-				-3.2
1,235,191	-				-1.2
1,235,238	-				-1.9
1,235,260	+				-2.1
1,235,260	+				-1.6
1,235,260	+				-2.3
1,235,260	+				+0.6
1,235,261	-				-2.5
1,235,261	-				-2.5
1,235,267	+				-1.4
1,235,267	+				+0.7
1,235,268	-				-3.2
1,235,268	-				-0.6
1,235,291	+				-3.2
1,235,291	+				-1.9
1,235,306	+				-3.4
1,235,352	-				-1.9
1,235,429	-		PP_1076	0.11	-2.0
1,235,767	+		PP_1076	0.50	-0.8
1,235,767	+		PP_1076	0.50	-1.0
1,235,857	+		PP_1076	0.61	-3.5
1,235,857	+		PP_1076	0.61	-2.2
1,235,857	+		PP_1076	0.61	-0.1
1,235,858	-		PP_1076	0.61	-0.6
1,235,858	-		PP_1076	0.61	-2.1
1,235,866	+		PP_1076	0.62	+0.1
1,235,898	+		PP_1076	0.66	-3.2
1,235,898	+		PP_1076	0.66	-1.3
1,235,898	+		PP_1076	0.66	-3.6
1,235,914	+		PP_1076	0.68	-3.3
1,235,914	+		PP_1076	0.68	-2.4
1,235,914	+		PP_1076	0.68	-2.7
1,235,914	+		PP_1076	0.68	-2.3
1,235,915	-		PP_1076	0.68	+1.7
1,235,915	-		PP_1076	0.68	-2.6
1,235,915	-		PP_1076	0.68	-3.0
1,236,010	+		PP_1076	0.79	-2.1
1,236,011	-		PP_1076	0.79	-0.7
1,236,011	-		PP_1076	0.79	-1.6
1,236,044	+		PP_1076	0.83	-2.1
1,236,045	-		PP_1076	0.83	-2.2
1,236,045	-		PP_1076	0.83	-1.5
1,236,190	+				-2.9
1,236,190	+				-2.5
1,236,190	+				-2.8
1,236,190	+				-2.7
1,236,190	+				-1.7
1,236,191	-				-0.6
1,236,191	-				-2.3
1,236,191	-				-3.5
1,236,191	-				-2.0
1,236,249	-				+1.7
1,236,272	+				-2.7
1,236,273	-				-2.5