Experiment: 3-methyl-1-butanol 0.1 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1022 and PP_1023 overlap by 29 nucleotides PP_1023 and PP_1024 overlap by 10 nucleotides PP_1024 and PP_1025 are separated by 252 nucleotides
PP_1022: PP_1022 - glucose 6-phosphate-1-dehydrogenase, at 1,165,616 to 1,167,085
_1022
PP_1023: PP_1023 - 6-phosphogluconolactonase, at 1,167,057 to 1,167,785
_1023
PP_1024: PP_1024 - KHG/KDPG aldolase, at 1,167,776 to 1,168,483
_1024
PP_1025: PP_1025 - 2-isopropylmalate synthase, at 1,168,736 to 1,170,409
_1025
Position (kb)
1167
1168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1166.141 kb on + strand, within PP_1022 at 1166.242 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.582 kb on - strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.716 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.871 kb on - strand, within PP_1022 at 1166.981 kb on - strand at 1166.981 kb on - strand at 1166.981 kb on - strand at 1167.079 kb on + strand at 1168.501 kb on - strand at 1168.547 kb on + strand at 1168.547 kb on + strand at 1168.548 kb on - strand at 1168.581 kb on - strand at 1168.592 kb on + strand at 1168.641 kb on - strand at 1168.643 kb on + strand at 1168.643 kb on + strand at 1168.644 kb on - strand at 1168.644 kb on - strand at 1168.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C) remove 1,166,141 + PP_1022 0.36 +2.4 1,166,242 + PP_1022 0.43 +0.4 1,166,471 + PP_1022 0.58 +1.3 1,166,471 + PP_1022 0.58 -2.6 1,166,582 - PP_1022 0.66 -0.6 1,166,681 + PP_1022 0.72 -2.5 1,166,681 + PP_1022 0.72 -1.5 1,166,681 + PP_1022 0.72 -1.8 1,166,681 + PP_1022 0.72 +2.3 1,166,681 + PP_1022 0.72 +3.3 1,166,716 + PP_1022 0.75 +0.3 1,166,870 + PP_1022 0.85 +1.6 1,166,870 + PP_1022 0.85 -0.1 1,166,871 - PP_1022 0.85 -0.9 1,166,981 - +1.7 1,166,981 - +0.7 1,166,981 - +0.1 1,167,079 + -1.8 1,168,501 - -0.2 1,168,547 + -0.6 1,168,547 + -3.5 1,168,548 - -0.9 1,168,581 - -0.1 1,168,592 + +2.3 1,168,641 - -2.3 1,168,643 + -0.8 1,168,643 + +0.8 1,168,644 - -0.5 1,168,644 - -0.5 1,168,644 - +0.1
Or see this region's nucleotide sequence