Experiment: 3-methyl-1-butanol 0.1 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0833 and PP_0834 are separated by 42 nucleotides PP_0834 and PP_0835 are separated by 63 nucleotides PP_0835 and PP_0836 are separated by 10 nucleotides
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645
_0834
PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571
_0835
PP_0836: PP_0836 - Sec translocation complex subunit SecF, at 973,582 to 974,490
_0836
Position (kb) 971
972
973
974 Strain fitness (log2 ratio) -2
-1
0
1 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833 at 971.201 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.639 kb on + strand at 974.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C) remove 970,716 + PP_0833 0.51 -1.8 970,955 + PP_0833 0.72 -1.2 970,985 + PP_0833 0.75 -1.8 971,060 + PP_0833 0.81 -2.0 971,085 + PP_0833 0.84 +0.0 971,135 + PP_0833 0.88 -1.4 971,201 + +0.2 971,298 + +0.6 971,298 + +1.2 971,298 + -0.5 971,418 + PP_0834 0.32 +1.0 971,418 + PP_0834 0.32 -0.1 971,418 + PP_0834 0.32 +0.1 971,639 + -0.3 974,488 + +1.0
Or see this region's nucleotide sequence