Experiment: 3-methyl-1-butanol 0.1 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0430 and PP_0431 are separated by 28 nucleotides PP_0431 and PP_0432 are separated by 142 nucleotides PP_0432 and PP_0433 are separated by 99 nucleotides PP_0433 and PP_0434 are separated by 59 nucleotides
PP_0430: PP_0430 - conserved protein of unknown function, at 517,059 to 518,015
_0430
PP_0431: PP_0431 - conserved membrane protein of unknown function, at 518,044 to 518,469
_0431
PP_0432: PP_0432 - N-acetyl-gamma-glutamyl-phosphate reductase 1, at 518,612 to 519,646
_0432
PP_0433: PP_0433 - Iron-sulfur cluster insertion protein ErpA, at 519,746 to 520,144
_0433
PP_0434: PP_0434 - Anhydro-N-acetylmuramic acid kinase, at 520,204 to 521,295
_0434
Position (kb) 518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 517.695 kb on + strand, within PP_0430 at 517.695 kb on + strand, within PP_0430 at 517.695 kb on + strand, within PP_0430 at 517.696 kb on - strand, within PP_0430 at 517.696 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.726 kb on - strand, within PP_0430 at 517.801 kb on + strand, within PP_0430 at 518.007 kb on + strand at 518.008 kb on - strand at 518.008 kb on - strand at 518.041 kb on - strand at 518.041 kb on - strand at 518.489 kb on - strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.505 kb on + strand at 518.506 kb on - strand at 518.506 kb on - strand at 518.506 kb on - strand at 518.508 kb on + strand at 518.508 kb on + strand at 518.508 kb on + strand at 518.509 kb on - strand at 518.509 kb on - strand at 518.509 kb on - strand at 518.546 kb on + strand at 518.547 kb on - strand at 518.566 kb on - strand at 518.568 kb on + strand at 518.569 kb on - strand at 518.598 kb on - strand at 518.611 kb on + strand at 518.747 kb on + strand, within PP_0432 at 518.747 kb on + strand, within PP_0432 at 518.748 kb on - strand, within PP_0432 at 518.768 kb on + strand, within PP_0432 at 518.768 kb on + strand, within PP_0432 at 518.951 kb on + strand, within PP_0432 at 518.996 kb on + strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 518.997 kb on - strand, within PP_0432 at 519.367 kb on + strand, within PP_0432 at 519.764 kb on + strand at 520.142 kb on + strand at 520.143 kb on - strand at 520.155 kb on + strand at 520.155 kb on + strand at 520.205 kb on + strand at 520.337 kb on - strand, within PP_0434 at 520.343 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.375 kb on + strand, within PP_0434 at 520.376 kb on - strand, within PP_0434
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C) remove 517,695 + PP_0430 0.66 +1.2 517,695 + PP_0430 0.66 +0.2 517,695 + PP_0430 0.66 +0.5 517,696 - PP_0430 0.67 -2.9 517,696 - PP_0430 0.67 -0.4 517,726 - PP_0430 0.70 +1.0 517,726 - PP_0430 0.70 +0.4 517,726 - PP_0430 0.70 +0.1 517,801 + PP_0430 0.78 -2.2 518,007 + +0.8 518,008 - -0.7 518,008 - -1.6 518,041 - +1.1 518,041 - +0.4 518,489 - -1.7 518,505 + +0.3 518,505 + -0.5 518,505 + -0.0 518,505 + +0.6 518,506 - -0.1 518,506 - -0.0 518,506 - -0.4 518,508 + +0.6 518,508 + +0.0 518,508 + +1.1 518,509 - +0.7 518,509 - +1.1 518,509 - -0.2 518,546 + +1.0 518,547 - +0.8 518,566 - +1.4 518,568 + +0.4 518,569 - +1.1 518,598 - +0.5 518,611 + -1.1 518,747 + PP_0432 0.13 +0.4 518,747 + PP_0432 0.13 +1.4 518,748 - PP_0432 0.13 +0.3 518,768 + PP_0432 0.15 -1.1 518,768 + PP_0432 0.15 +1.6 518,951 + PP_0432 0.33 +0.0 518,996 + PP_0432 0.37 +2.0 518,997 - PP_0432 0.37 +1.3 518,997 - PP_0432 0.37 +1.8 518,997 - PP_0432 0.37 +0.8 519,367 + PP_0432 0.73 +1.6 519,764 + +0.6 520,142 + -0.3 520,143 - -1.2 520,155 + -0.2 520,155 + -0.2 520,205 + -0.7 520,337 - PP_0434 0.12 -0.4 520,343 + PP_0434 0.13 -1.3 520,375 + PP_0434 0.16 -0.5 520,375 + PP_0434 0.16 -0.7 520,376 - PP_0434 0.16 -0.9
Or see this region's nucleotide sequence