Experiment: 3-methyl-1-butanol 0.1 vol% (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0363 and PP_0364 overlap by 8 nucleotides PP_0364 and PP_0365 overlap by 8 nucleotides PP_0365 and PP_0366 are separated by 10 nucleotides PP_0366 and PP_0367 are separated by 173 nucleotides
PP_0363: PP_0363 - 8-amino-7-oxononanoate synthase, at 441,120 to 442,292
_0363
PP_0364: PP_0364 - pimeloyl-[acp] methyl ester esterase, at 442,285 to 443,016
_0364
PP_0365: PP_0365 - Malonyl-[acyl-carrier protein] O-methyltransferase, at 443,009 to 443,827
_0365
PP_0366: PP_0366 - dethiobiotin synthetase, at 443,838 to 444,518
_0366
PP_0367: PP_0367 - conserved protein of unknown function, at 444,692 to 445,027
_0367
Position (kb) 443
444 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 442.237 kb on + strand at 442.238 kb on - strand at 442.238 kb on - strand at 442.238 kb on - strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.291 kb on - strand at 442.304 kb on + strand at 442.304 kb on + strand at 442.305 kb on - strand at 442.305 kb on - strand at 442.305 kb on - strand at 442.385 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.660 kb on - strand, within PP_0364 at 442.677 kb on + strand, within PP_0364 at 442.677 kb on + strand, within PP_0364 at 442.678 kb on - strand, within PP_0364 at 442.759 kb on + strand, within PP_0364 at 442.759 kb on + strand, within PP_0364 at 442.788 kb on + strand, within PP_0364 at 442.819 kb on + strand, within PP_0364 at 442.819 kb on + strand, within PP_0364 at 442.862 kb on + strand, within PP_0364 at 442.863 kb on - strand, within PP_0364 at 442.911 kb on - strand, within PP_0364 at 442.911 kb on - strand, within PP_0364 at 442.957 kb on + strand at 443.097 kb on - strand, within PP_0365 at 443.127 kb on + strand, within PP_0365 at 443.312 kb on + strand, within PP_0365 at 443.312 kb on + strand, within PP_0365 at 443.313 kb on - strand, within PP_0365 at 443.455 kb on - strand, within PP_0365 at 443.455 kb on - strand, within PP_0365 at 443.535 kb on + strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.561 kb on + strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.622 kb on + strand, within PP_0365 at 443.622 kb on + strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.787 kb on - strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.826 kb on - strand at 444.043 kb on - strand, within PP_0366 at 444.043 kb on - strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.078 kb on - strand, within PP_0366 at 444.080 kb on - strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.559 kb on + strand at 444.594 kb on - strand at 444.630 kb on + strand at 444.631 kb on - strand at 444.659 kb on + strand at 444.659 kb on + strand at 444.679 kb on + strand at 444.690 kb on - strand at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.759 kb on - strand, within PP_0367
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 0.1 vol% (C) remove 442,237 + -3.9 442,238 - -3.9 442,238 - -3.0 442,238 - -1.8 442,290 + -1.5 442,290 + -2.5 442,291 - -2.0 442,304 + -3.2 442,304 + -3.5 442,305 - -2.2 442,305 - -0.6 442,305 - -1.2 442,385 + PP_0364 0.14 -3.9 442,650 + PP_0364 0.50 -4.1 442,650 + PP_0364 0.50 -3.8 442,650 + PP_0364 0.50 -4.2 442,651 - PP_0364 0.50 -2.6 442,651 - PP_0364 0.50 -2.2 442,651 - PP_0364 0.50 -3.6 442,651 - PP_0364 0.50 -2.1 442,660 - PP_0364 0.51 -3.5 442,677 + PP_0364 0.54 -3.0 442,677 + PP_0364 0.54 -3.2 442,678 - PP_0364 0.54 -4.3 442,759 + PP_0364 0.65 -1.9 442,759 + PP_0364 0.65 -3.2 442,788 + PP_0364 0.69 +0.3 442,819 + PP_0364 0.73 -3.0 442,819 + PP_0364 0.73 -1.8 442,862 + PP_0364 0.79 -4.2 442,863 - PP_0364 0.79 -3.0 442,911 - PP_0364 0.86 -0.8 442,911 - PP_0364 0.86 -4.8 442,957 + -1.7 443,097 - PP_0365 0.11 -2.9 443,127 + PP_0365 0.14 -2.3 443,312 + PP_0365 0.37 -3.3 443,312 + PP_0365 0.37 -4.1 443,313 - PP_0365 0.37 -2.9 443,455 - PP_0365 0.54 -3.8 443,455 - PP_0365 0.54 -1.7 443,535 + PP_0365 0.64 -4.0 443,536 - PP_0365 0.64 -2.0 443,536 - PP_0365 0.64 -1.5 443,536 - PP_0365 0.64 -2.3 443,561 + PP_0365 0.67 -3.8 443,562 - PP_0365 0.68 -4.4 443,562 - PP_0365 0.68 -4.3 443,562 - PP_0365 0.68 -3.8 443,622 + PP_0365 0.75 -2.4 443,622 + PP_0365 0.75 -2.3 443,623 - PP_0365 0.75 +1.3 443,623 - PP_0365 0.75 -3.1 443,623 - PP_0365 0.75 -4.4 443,623 - PP_0365 0.75 -1.4 443,623 - PP_0365 0.75 -1.5 443,623 - PP_0365 0.75 -3.1 443,623 - PP_0365 0.75 -2.7 443,623 - PP_0365 0.75 -2.9 443,623 - PP_0365 0.75 -3.2 443,623 - PP_0365 0.75 -2.8 443,623 - PP_0365 0.75 -3.1 443,747 + -1.1 443,747 + -4.5 443,747 + -0.9 443,747 + -3.8 443,747 + -2.4 443,748 - -1.7 443,748 - -2.9 443,748 - -3.3 443,748 - -4.2 443,786 + -4.2 443,786 + -0.9 443,786 + -4.0 443,787 - -4.1 443,798 + -3.7 443,798 + -3.8 443,798 + -3.0 443,798 + -3.5 443,798 + -3.4 443,798 + -3.6 443,799 - -3.8 443,799 - -3.7 443,799 - -3.9 443,799 - -3.3 443,799 - -3.1 443,799 - -2.4 443,826 - -2.8 444,043 - PP_0366 0.30 -2.6 444,043 - PP_0366 0.30 -4.0 444,077 + PP_0366 0.35 -2.6 444,077 + PP_0366 0.35 -4.7 444,078 - PP_0366 0.35 -0.7 444,080 - PP_0366 0.36 -5.3 444,184 + PP_0366 0.51 -2.1 444,184 + PP_0366 0.51 -4.2 444,185 - PP_0366 0.51 -4.8 444,185 - PP_0366 0.51 -3.8 444,185 - PP_0366 0.51 -2.0 444,517 - +0.5 444,517 - +0.3 444,517 - +0.3 444,517 - -0.8 444,559 + +0.5 444,594 - +0.8 444,630 + +1.7 444,631 - +0.7 444,659 + -0.1 444,659 + -0.7 444,679 + +0.1 444,690 - -2.4 444,758 + PP_0367 0.20 -0.3 444,758 + PP_0367 0.20 -1.1 444,758 + PP_0367 0.20 +1.1 444,758 + PP_0367 0.20 +0.2 444,758 + PP_0367 0.20 -1.1 444,759 - PP_0367 0.20 -1.6 444,759 - PP_0367 0.20 -0.7
Or see this region's nucleotide sequence