Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4740 and Psyr_4741 are separated by 51 nucleotides Psyr_4741 and Psyr_4742 are separated by 114 nucleotides Psyr_4742 and Psyr_4743 are separated by 80 nucleotides Psyr_4743 and Psyr_4744 are separated by 147 nucleotides Psyr_4744 and Psyr_4745 are separated by 148 nucleotides
Psyr_4740: Psyr_4740 - thiazole-phosphate synthase, at 5,619,406 to 5,620,200
_4740
Psyr_4741: Psyr_4741 - sulfur carrier protein ThiS, at 5,620,252 to 5,620,452
_4741
Psyr_4742: Psyr_4742 - Protein of unknown function DUF423, at 5,620,567 to 5,620,947
_4742
Psyr_4743: Psyr_4743 - Glycosyl transferase, family 51, at 5,621,028 to 5,621,738
_4743
Psyr_4744: Psyr_4744 - hypothetical protein, at 5,621,886 to 5,622,131
_4744
Psyr_4745: Psyr_4745 - hypothetical protein, at 5,622,280 to 5,622,621
_4745
Position (kb) 5621
5622 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5620.118 kb on + strand, within Psyr_4740 at 5620.118 kb on + strand, within Psyr_4740 at 5620.118 kb on + strand, within Psyr_4740 at 5620.118 kb on + strand, within Psyr_4740 at 5620.118 kb on + strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.119 kb on - strand, within Psyr_4740 at 5620.213 kb on + strand at 5620.213 kb on + strand at 5620.253 kb on + strand at 5620.254 kb on - strand at 5620.489 kb on + strand at 5620.713 kb on + strand, within Psyr_4742 at 5620.714 kb on - strand, within Psyr_4742 at 5620.814 kb on + strand, within Psyr_4742 at 5620.845 kb on - strand, within Psyr_4742 at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.989 kb on + strand at 5620.990 kb on - strand at 5620.990 kb on - strand at 5620.990 kb on - strand at 5620.990 kb on - strand at 5620.990 kb on - strand at 5620.990 kb on - strand at 5620.992 kb on - strand at 5620.992 kb on - strand at 5621.027 kb on + strand at 5621.027 kb on + strand at 5621.028 kb on - strand at 5621.028 kb on - strand at 5621.028 kb on - strand at 5621.039 kb on + strand at 5621.039 kb on + strand at 5621.039 kb on + strand at 5621.040 kb on - strand at 5621.155 kb on + strand, within Psyr_4743 at 5621.156 kb on - strand, within Psyr_4743 at 5621.156 kb on - strand, within Psyr_4743 at 5621.156 kb on - strand, within Psyr_4743 at 5621.156 kb on - strand, within Psyr_4743 at 5621.403 kb on + strand, within Psyr_4743 at 5621.403 kb on + strand, within Psyr_4743 at 5621.404 kb on - strand, within Psyr_4743 at 5621.449 kb on + strand, within Psyr_4743 at 5621.449 kb on + strand, within Psyr_4743 at 5621.449 kb on + strand, within Psyr_4743 at 5621.449 kb on + strand, within Psyr_4743 at 5621.449 kb on + strand, within Psyr_4743 at 5621.450 kb on - strand, within Psyr_4743 at 5621.450 kb on - strand, within Psyr_4743 at 5621.450 kb on - strand, within Psyr_4743 at 5621.481 kb on + strand, within Psyr_4743 at 5621.482 kb on - strand, within Psyr_4743 at 5621.482 kb on - strand, within Psyr_4743 at 5621.482 kb on - strand, within Psyr_4743 at 5621.482 kb on - strand, within Psyr_4743 at 5621.482 kb on - strand, within Psyr_4743 at 5621.586 kb on + strand, within Psyr_4743 at 5621.586 kb on + strand, within Psyr_4743 at 5621.587 kb on - strand, within Psyr_4743 at 5621.644 kb on - strand, within Psyr_4743 at 5621.644 kb on - strand, within Psyr_4743 at 5621.644 kb on - strand, within Psyr_4743 at 5621.703 kb on + strand at 5621.704 kb on - strand at 5621.773 kb on + strand at 5621.774 kb on - strand at 5621.774 kb on - strand at 5621.774 kb on - strand at 5621.774 kb on - strand at 5621.802 kb on - strand at 5621.802 kb on - strand at 5621.919 kb on + strand, within Psyr_4744 at 5621.919 kb on + strand, within Psyr_4744 at 5621.919 kb on + strand, within Psyr_4744 at 5622.501 kb on + strand, within Psyr_4745 at 5622.501 kb on + strand, within Psyr_4745 at 5622.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 5,620,118 + Psyr_4740 0.90 -3.7 5,620,118 + Psyr_4740 0.90 -3.4 5,620,118 + Psyr_4740 0.90 -0.6 5,620,118 + Psyr_4740 0.90 -3.4 5,620,118 + Psyr_4740 0.90 -0.1 5,620,119 - Psyr_4740 0.90 -2.7 5,620,119 - Psyr_4740 0.90 -3.4 5,620,119 - Psyr_4740 0.90 -2.6 5,620,119 - Psyr_4740 0.90 -2.0 5,620,119 - Psyr_4740 0.90 -2.2 5,620,119 - Psyr_4740 0.90 -2.2 5,620,119 - Psyr_4740 0.90 -2.2 5,620,119 - Psyr_4740 0.90 -0.2 5,620,213 + -0.3 5,620,213 + -1.2 5,620,253 + +0.7 5,620,254 - -0.5 5,620,489 + -2.4 5,620,713 + Psyr_4742 0.38 +0.9 5,620,714 - Psyr_4742 0.39 -0.4 5,620,814 + Psyr_4742 0.65 -0.2 5,620,845 - Psyr_4742 0.73 +0.3 5,620,989 + -0.0 5,620,989 + -0.4 5,620,989 + -0.7 5,620,989 + +0.2 5,620,989 + +0.8 5,620,989 + -0.4 5,620,989 + +0.4 5,620,989 + -1.0 5,620,989 + -0.7 5,620,990 - +0.8 5,620,990 - -0.5 5,620,990 - -1.8 5,620,990 - +0.2 5,620,990 - +0.2 5,620,990 - +0.8 5,620,992 - -0.4 5,620,992 - -1.0 5,621,027 + +1.0 5,621,027 + -0.5 5,621,028 - +3.7 5,621,028 - -0.7 5,621,028 - -1.0 5,621,039 + -0.5 5,621,039 + -0.6 5,621,039 + +0.2 5,621,040 - -2.1 5,621,155 + Psyr_4743 0.18 -0.3 5,621,156 - Psyr_4743 0.18 -0.4 5,621,156 - Psyr_4743 0.18 +0.4 5,621,156 - Psyr_4743 0.18 -0.3 5,621,156 - Psyr_4743 0.18 -0.5 5,621,403 + Psyr_4743 0.53 +1.8 5,621,403 + Psyr_4743 0.53 -0.8 5,621,404 - Psyr_4743 0.53 -0.1 5,621,449 + Psyr_4743 0.59 -1.2 5,621,449 + Psyr_4743 0.59 +1.1 5,621,449 + Psyr_4743 0.59 +0.8 5,621,449 + Psyr_4743 0.59 +1.3 5,621,449 + Psyr_4743 0.59 -2.8 5,621,450 - Psyr_4743 0.59 -0.3 5,621,450 - Psyr_4743 0.59 -1.1 5,621,450 - Psyr_4743 0.59 -0.7 5,621,481 + Psyr_4743 0.64 -0.6 5,621,482 - Psyr_4743 0.64 +1.3 5,621,482 - Psyr_4743 0.64 -1.2 5,621,482 - Psyr_4743 0.64 +1.1 5,621,482 - Psyr_4743 0.64 -0.8 5,621,482 - Psyr_4743 0.64 +1.6 5,621,586 + Psyr_4743 0.78 +0.8 5,621,586 + Psyr_4743 0.78 -0.4 5,621,587 - Psyr_4743 0.79 +0.6 5,621,644 - Psyr_4743 0.87 -1.1 5,621,644 - Psyr_4743 0.87 -0.8 5,621,644 - Psyr_4743 0.87 +1.3 5,621,703 + +1.0 5,621,704 - -0.2 5,621,773 + +0.3 5,621,774 - -1.4 5,621,774 - -0.5 5,621,774 - +0.2 5,621,774 - +3.3 5,621,802 - +1.9 5,621,802 - -0.0 5,621,919 + Psyr_4744 0.13 -1.3 5,621,919 + Psyr_4744 0.13 +0.3 5,621,919 + Psyr_4744 0.13 -0.7 5,622,501 + Psyr_4745 0.65 -0.1 5,622,501 + Psyr_4745 0.65 -0.7 5,622,737 + -0.6
Or see this region's nucleotide sequence