Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3661 and Psyr_3662 overlap by 8 nucleotides Psyr_3662 and Psyr_3663 are separated by 20 nucleotides Psyr_3663 and Psyr_3664 are separated by 100 nucleotides Psyr_3664 and Psyr_3665 are separated by 226 nucleotides
Psyr_3661: Psyr_3661 - Fumarate hydratase, class II, at 4,347,509 to 4,348,903
_3661
Psyr_3662: Psyr_3662 - NAD(P)H dehydrogenase (quinone), at 4,348,896 to 4,349,510
_3662
Psyr_3663: Psyr_3663 - Protein of unknown function DUF6, at 4,349,531 to 4,350,490
_3663
Psyr_3664: Psyr_3664 - DTW, at 4,350,591 to 4,351,190
_3664
Psyr_3665: Psyr_3665 - conserved hypothetical protein, at 4,351,417 to 4,351,719
_3665
Position (kb) 4349
4350
4351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4348.583 kb on + strand, within Psyr_3661 at 4348.583 kb on + strand, within Psyr_3661 at 4348.584 kb on - strand, within Psyr_3661 at 4348.584 kb on - strand, within Psyr_3661 at 4348.787 kb on + strand at 4348.788 kb on - strand at 4349.341 kb on - strand, within Psyr_3662 at 4349.356 kb on - strand, within Psyr_3662 at 4349.392 kb on + strand, within Psyr_3662 at 4349.411 kb on - strand, within Psyr_3662 at 4349.411 kb on - strand, within Psyr_3662 at 4349.901 kb on + strand, within Psyr_3663 at 4349.901 kb on + strand, within Psyr_3663 at 4349.915 kb on + strand, within Psyr_3663 at 4349.916 kb on - strand, within Psyr_3663 at 4349.934 kb on - strand, within Psyr_3663 at 4350.015 kb on + strand, within Psyr_3663 at 4350.016 kb on - strand, within Psyr_3663 at 4350.219 kb on + strand, within Psyr_3663 at 4350.220 kb on - strand, within Psyr_3663 at 4350.220 kb on - strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.228 kb on + strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.229 kb on - strand, within Psyr_3663 at 4350.462 kb on + strand at 4350.463 kb on - strand at 4350.509 kb on + strand at 4350.509 kb on + strand at 4350.509 kb on + strand at 4350.510 kb on - strand at 4350.529 kb on - strand at 4350.554 kb on + strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.555 kb on - strand at 4350.619 kb on + strand at 4350.619 kb on + strand at 4350.913 kb on + strand, within Psyr_3664 at 4350.914 kb on - strand, within Psyr_3664 at 4350.914 kb on - strand, within Psyr_3664 at 4351.199 kb on - strand at 4351.376 kb on + strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.377 kb on - strand at 4351.387 kb on + strand at 4351.387 kb on + strand at 4351.458 kb on - strand, within Psyr_3665 at 4351.458 kb on - strand, within Psyr_3665 at 4351.469 kb on + strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665 at 4351.470 kb on - strand, within Psyr_3665
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 4,348,583 + Psyr_3661 0.77 +0.9 4,348,583 + Psyr_3661 0.77 -0.0 4,348,584 - Psyr_3661 0.77 +1.7 4,348,584 - Psyr_3661 0.77 -0.0 4,348,787 + +1.2 4,348,788 - -0.0 4,349,341 - Psyr_3662 0.72 +4.0 4,349,356 - Psyr_3662 0.75 +2.8 4,349,392 + Psyr_3662 0.81 -0.5 4,349,411 - Psyr_3662 0.84 -0.1 4,349,411 - Psyr_3662 0.84 -1.0 4,349,901 + Psyr_3663 0.39 -0.5 4,349,901 + Psyr_3663 0.39 -0.8 4,349,915 + Psyr_3663 0.40 -0.7 4,349,916 - Psyr_3663 0.40 -0.4 4,349,934 - Psyr_3663 0.42 +1.3 4,350,015 + Psyr_3663 0.50 +2.2 4,350,016 - Psyr_3663 0.51 -0.6 4,350,219 + Psyr_3663 0.72 +1.5 4,350,220 - Psyr_3663 0.72 -0.1 4,350,220 - Psyr_3663 0.72 -0.5 4,350,228 + Psyr_3663 0.73 +1.3 4,350,228 + Psyr_3663 0.73 +0.0 4,350,228 + Psyr_3663 0.73 -0.2 4,350,228 + Psyr_3663 0.73 +0.6 4,350,228 + Psyr_3663 0.73 +0.7 4,350,228 + Psyr_3663 0.73 +0.2 4,350,228 + Psyr_3663 0.73 -3.8 4,350,228 + Psyr_3663 0.73 +0.9 4,350,228 + Psyr_3663 0.73 +1.9 4,350,228 + Psyr_3663 0.73 +0.6 4,350,228 + Psyr_3663 0.73 -0.5 4,350,228 + Psyr_3663 0.73 -0.7 4,350,228 + Psyr_3663 0.73 -0.5 4,350,229 - Psyr_3663 0.73 +0.5 4,350,229 - Psyr_3663 0.73 +0.0 4,350,229 - Psyr_3663 0.73 -0.8 4,350,229 - Psyr_3663 0.73 +0.4 4,350,229 - Psyr_3663 0.73 +1.1 4,350,229 - Psyr_3663 0.73 -1.4 4,350,229 - Psyr_3663 0.73 +2.5 4,350,229 - Psyr_3663 0.73 +0.3 4,350,229 - Psyr_3663 0.73 +0.5 4,350,229 - Psyr_3663 0.73 -1.4 4,350,462 + +0.6 4,350,463 - +0.8 4,350,509 + +0.2 4,350,509 + +0.4 4,350,509 + -0.1 4,350,510 - -1.6 4,350,529 - +0.3 4,350,554 + +0.2 4,350,555 - -0.8 4,350,555 - -1.0 4,350,555 - -0.5 4,350,555 - +0.2 4,350,619 + -1.7 4,350,619 + +0.6 4,350,913 + Psyr_3664 0.54 +0.3 4,350,914 - Psyr_3664 0.54 +3.8 4,350,914 - Psyr_3664 0.54 +1.6 4,351,199 - +0.0 4,351,376 + -0.1 4,351,377 - -2.3 4,351,377 - -0.4 4,351,377 - +1.9 4,351,377 - -0.9 4,351,377 - -0.1 4,351,377 - +1.6 4,351,387 + -0.9 4,351,387 + -1.3 4,351,458 - Psyr_3665 0.14 -2.4 4,351,458 - Psyr_3665 0.14 +0.9 4,351,469 + Psyr_3665 0.17 -2.3 4,351,470 - Psyr_3665 0.17 +0.1 4,351,470 - Psyr_3665 0.17 +0.3 4,351,470 - Psyr_3665 0.17 +0.2
Or see this region's nucleotide sequence