Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3546 and Psyr_3547 are separated by 21 nucleotides Psyr_3547 and Psyr_3548 are separated by 210 nucleotides Psyr_3548 and Psyr_3549 are separated by 335 nucleotides
Psyr_3546: Psyr_3546 - transcriptional regulator, AraC family, at 4,223,650 to 4,224,558
_3546
Psyr_3547: Psyr_3547 - transcriptional regulator, TetR family, at 4,224,580 to 4,225,143
_3547
Psyr_3548: Psyr_3548 - Glutathione S-transferase, N-terminal, at 4,225,354 to 4,226,055
_3548
Psyr_3549: Psyr_3549 - MscS Mechanosensitive ion channel, at 4,226,391 to 4,228,802
_3549
Position (kb) 4225
4226
4227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4224.540 kb on + strand at 4224.540 kb on + strand at 4224.541 kb on - strand at 4224.608 kb on + strand at 4224.609 kb on - strand at 4224.609 kb on - strand at 4224.781 kb on + strand, within Psyr_3547 at 4224.781 kb on + strand, within Psyr_3547 at 4224.782 kb on - strand, within Psyr_3547 at 4225.016 kb on + strand, within Psyr_3547 at 4225.017 kb on - strand, within Psyr_3547 at 4225.017 kb on - strand, within Psyr_3547 at 4225.019 kb on + strand, within Psyr_3547 at 4225.019 kb on + strand, within Psyr_3547 at 4225.019 kb on + strand, within Psyr_3547 at 4225.019 kb on + strand, within Psyr_3547 at 4225.020 kb on - strand, within Psyr_3547 at 4225.023 kb on + strand, within Psyr_3547 at 4225.024 kb on - strand, within Psyr_3547 at 4225.079 kb on + strand, within Psyr_3547 at 4225.080 kb on - strand, within Psyr_3547 at 4225.146 kb on + strand at 4225.147 kb on - strand at 4225.157 kb on - strand at 4225.157 kb on - strand at 4225.157 kb on - strand at 4225.190 kb on + strand at 4225.190 kb on + strand at 4225.190 kb on + strand at 4225.190 kb on + strand at 4225.190 kb on + strand at 4225.191 kb on - strand at 4225.191 kb on - strand at 4225.191 kb on - strand at 4225.191 kb on - strand at 4225.214 kb on - strand at 4225.217 kb on + strand at 4225.218 kb on - strand at 4225.331 kb on - strand at 4225.403 kb on + strand at 4225.404 kb on - strand at 4225.446 kb on - strand, within Psyr_3548 at 4225.723 kb on - strand, within Psyr_3548 at 4225.723 kb on - strand, within Psyr_3548 at 4225.753 kb on - strand, within Psyr_3548 at 4225.943 kb on + strand, within Psyr_3548 at 4225.943 kb on + strand, within Psyr_3548 at 4225.944 kb on - strand, within Psyr_3548 at 4225.944 kb on - strand, within Psyr_3548 at 4226.073 kb on - strand at 4226.115 kb on + strand at 4226.169 kb on + strand at 4226.169 kb on + strand at 4226.195 kb on + strand at 4226.196 kb on - strand at 4226.281 kb on + strand at 4226.421 kb on - strand at 4226.818 kb on + strand, within Psyr_3549 at 4226.818 kb on + strand, within Psyr_3549 at 4226.818 kb on + strand, within Psyr_3549 at 4226.818 kb on + strand, within Psyr_3549 at 4226.819 kb on - strand, within Psyr_3549 at 4226.819 kb on - strand, within Psyr_3549 at 4226.819 kb on - strand, within Psyr_3549 at 4226.819 kb on - strand, within Psyr_3549 at 4226.851 kb on + strand, within Psyr_3549 at 4226.852 kb on - strand, within Psyr_3549 at 4226.990 kb on - strand, within Psyr_3549
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 4,224,540 + -0.9 4,224,540 + +0.1 4,224,541 - +1.5 4,224,608 + -1.2 4,224,609 - -1.7 4,224,609 - +1.0 4,224,781 + Psyr_3547 0.36 -0.2 4,224,781 + Psyr_3547 0.36 -0.9 4,224,782 - Psyr_3547 0.36 -0.5 4,225,016 + Psyr_3547 0.77 -1.6 4,225,017 - Psyr_3547 0.77 -0.6 4,225,017 - Psyr_3547 0.77 -0.8 4,225,019 + Psyr_3547 0.78 -0.7 4,225,019 + Psyr_3547 0.78 -1.6 4,225,019 + Psyr_3547 0.78 -0.4 4,225,019 + Psyr_3547 0.78 +2.7 4,225,020 - Psyr_3547 0.78 -0.9 4,225,023 + Psyr_3547 0.79 -1.1 4,225,024 - Psyr_3547 0.79 -0.0 4,225,079 + Psyr_3547 0.88 -0.9 4,225,080 - Psyr_3547 0.89 +0.3 4,225,146 + -0.9 4,225,147 - -0.1 4,225,157 - +2.3 4,225,157 - -0.3 4,225,157 - +1.1 4,225,190 + +0.3 4,225,190 + -0.9 4,225,190 + +0.2 4,225,190 + -0.1 4,225,190 + -3.2 4,225,191 - -0.2 4,225,191 - -0.5 4,225,191 - -0.3 4,225,191 - -0.3 4,225,214 - -0.0 4,225,217 + -0.3 4,225,218 - -0.8 4,225,331 - +0.3 4,225,403 + -1.2 4,225,404 - +0.7 4,225,446 - Psyr_3548 0.13 +0.0 4,225,723 - Psyr_3548 0.53 +0.1 4,225,723 - Psyr_3548 0.53 -1.7 4,225,753 - Psyr_3548 0.57 +0.0 4,225,943 + Psyr_3548 0.84 -1.4 4,225,943 + Psyr_3548 0.84 -2.2 4,225,944 - Psyr_3548 0.84 +0.8 4,225,944 - Psyr_3548 0.84 -0.4 4,226,073 - -1.8 4,226,115 + +0.0 4,226,169 + +0.0 4,226,169 + +1.5 4,226,195 + -0.6 4,226,196 - -0.2 4,226,281 + -0.2 4,226,421 - +0.3 4,226,818 + Psyr_3549 0.18 +1.4 4,226,818 + Psyr_3549 0.18 -1.0 4,226,818 + Psyr_3549 0.18 -0.5 4,226,818 + Psyr_3549 0.18 +3.5 4,226,819 - Psyr_3549 0.18 -0.7 4,226,819 - Psyr_3549 0.18 -0.1 4,226,819 - Psyr_3549 0.18 +3.8 4,226,819 - Psyr_3549 0.18 +0.1 4,226,851 + Psyr_3549 0.19 -0.9 4,226,852 - Psyr_3549 0.19 +0.0 4,226,990 - Psyr_3549 0.25 -0.3
Or see this region's nucleotide sequence