Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3431 and Psyr_3432 are separated by 6 nucleotides Psyr_3432 and Psyr_3433 overlap by 1 nucleotides Psyr_3433 and Psyr_3434 are separated by 50 nucleotides
Psyr_3431: Psyr_3431 - OmpA/MotB, at 4,093,985 to 4,094,872
_3431
Psyr_3432: Psyr_3432 - MotA/TolQ/ExbB proton channel, at 4,094,879 to 4,095,619
_3432
Psyr_3433: Psyr_3433 - Protein-glutamate methylesterase, at 4,095,619 to 4,096,779
_3433
Psyr_3434: Psyr_3434 - CheW-like protein:ATP-binding region, ATPase-like:Signal transducing histidine kinase, homodimeric:Hpt, at 4,096,830 to 4,099,106
_3434
Position (kb)
4095
4096
4097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4094.739 kb on + strand, within Psyr_3431 at 4094.739 kb on + strand, within Psyr_3431 at 4094.739 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.770 kb on - strand, within Psyr_3431 at 4094.770 kb on - strand, within Psyr_3431 at 4094.796 kb on - strand at 4094.796 kb on - strand at 4094.800 kb on + strand at 4094.800 kb on + strand at 4094.801 kb on - strand at 4094.814 kb on + strand at 4094.814 kb on + strand at 4095.048 kb on + strand, within Psyr_3432 at 4095.048 kb on + strand, within Psyr_3432 at 4095.049 kb on - strand, within Psyr_3432 at 4095.049 kb on - strand, within Psyr_3432 at 4095.049 kb on - strand, within Psyr_3432 at 4095.076 kb on + strand, within Psyr_3432 at 4095.406 kb on + strand, within Psyr_3432 at 4095.407 kb on - strand, within Psyr_3432 at 4095.407 kb on - strand, within Psyr_3432 at 4095.407 kb on - strand, within Psyr_3432 at 4095.407 kb on - strand, within Psyr_3432 at 4095.611 kb on + strand at 4095.611 kb on + strand at 4095.611 kb on + strand at 4095.612 kb on - strand at 4095.612 kb on - strand at 4095.612 kb on - strand at 4095.612 kb on - strand at 4095.635 kb on - strand at 4095.665 kb on + strand at 4095.666 kb on - strand at 4095.666 kb on - strand at 4095.666 kb on - strand at 4095.666 kb on - strand at 4095.713 kb on + strand at 4095.714 kb on + strand at 4095.881 kb on + strand, within Psyr_3433 at 4095.881 kb on + strand, within Psyr_3433 at 4095.882 kb on - strand, within Psyr_3433 at 4095.882 kb on - strand, within Psyr_3433 at 4095.982 kb on + strand, within Psyr_3433 at 4095.982 kb on + strand, within Psyr_3433 at 4095.982 kb on + strand, within Psyr_3433 at 4095.983 kb on - strand, within Psyr_3433 at 4096.176 kb on - strand, within Psyr_3433 at 4096.386 kb on + strand, within Psyr_3433 at 4096.386 kb on + strand, within Psyr_3433 at 4096.387 kb on - strand, within Psyr_3433 at 4096.449 kb on + strand, within Psyr_3433 at 4096.449 kb on + strand, within Psyr_3433 at 4096.693 kb on + strand at 4096.694 kb on - strand at 4096.694 kb on - strand at 4096.791 kb on + strand at 4096.791 kb on + strand at 4096.791 kb on + strand at 4096.792 kb on - strand at 4096.792 kb on - strand at 4096.835 kb on + strand at 4096.836 kb on - strand at 4096.932 kb on - strand at 4097.118 kb on + strand, within Psyr_3434 at 4097.119 kb on - strand, within Psyr_3434 at 4097.119 kb on - strand, within Psyr_3434 at 4097.302 kb on + strand, within Psyr_3434 at 4097.302 kb on + strand, within Psyr_3434 at 4097.302 kb on + strand, within Psyr_3434 at 4097.302 kb on + strand, within Psyr_3434 at 4097.303 kb on - strand, within Psyr_3434 at 4097.303 kb on - strand, within Psyr_3434 at 4097.306 kb on + strand, within Psyr_3434 at 4097.426 kb on - strand, within Psyr_3434 at 4097.426 kb on - strand, within Psyr_3434 at 4097.426 kb on - strand, within Psyr_3434 at 4097.648 kb on + strand, within Psyr_3434
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 4,094,739 + Psyr_3431 0.85 -0.6 4,094,739 + Psyr_3431 0.85 -0.3 4,094,739 + Psyr_3431 0.85 +0.5 4,094,769 + Psyr_3431 0.88 -0.5 4,094,769 + Psyr_3431 0.88 -0.5 4,094,769 + Psyr_3431 0.88 +4.1 4,094,770 - Psyr_3431 0.88 +2.1 4,094,770 - Psyr_3431 0.88 +1.2 4,094,796 - -0.7 4,094,796 - -1.3 4,094,800 + -0.7 4,094,800 + +1.6 4,094,801 - -0.6 4,094,814 + -1.1 4,094,814 + -1.0 4,095,048 + Psyr_3432 0.23 -1.5 4,095,048 + Psyr_3432 0.23 +0.4 4,095,049 - Psyr_3432 0.23 -0.2 4,095,049 - Psyr_3432 0.23 -0.2 4,095,049 - Psyr_3432 0.23 +0.8 4,095,076 + Psyr_3432 0.27 -0.9 4,095,406 + Psyr_3432 0.71 -1.7 4,095,407 - Psyr_3432 0.71 -0.2 4,095,407 - Psyr_3432 0.71 -0.9 4,095,407 - Psyr_3432 0.71 -0.1 4,095,407 - Psyr_3432 0.71 +0.0 4,095,611 + +1.0 4,095,611 + +0.4 4,095,611 + -0.0 4,095,612 - -1.2 4,095,612 - +0.1 4,095,612 - +3.4 4,095,612 - -0.1 4,095,635 - -0.2 4,095,665 + -2.8 4,095,666 - -0.5 4,095,666 - +0.1 4,095,666 - -0.9 4,095,666 - -1.5 4,095,713 + +0.3 4,095,714 + +0.3 4,095,881 + Psyr_3433 0.23 +1.1 4,095,881 + Psyr_3433 0.23 -1.7 4,095,882 - Psyr_3433 0.23 -1.7 4,095,882 - Psyr_3433 0.23 -2.6 4,095,982 + Psyr_3433 0.31 +0.4 4,095,982 + Psyr_3433 0.31 +1.7 4,095,982 + Psyr_3433 0.31 -0.3 4,095,983 - Psyr_3433 0.31 -1.3 4,096,176 - Psyr_3433 0.48 -1.0 4,096,386 + Psyr_3433 0.66 -0.6 4,096,386 + Psyr_3433 0.66 -1.4 4,096,387 - Psyr_3433 0.66 -1.1 4,096,449 + Psyr_3433 0.71 +0.5 4,096,449 + Psyr_3433 0.71 -0.7 4,096,693 + -0.1 4,096,694 - -1.4 4,096,694 - -1.0 4,096,791 + +0.9 4,096,791 + -0.9 4,096,791 + +0.7 4,096,792 - -1.2 4,096,792 - +1.6 4,096,835 + -2.2 4,096,836 - -0.5 4,096,932 - -0.3 4,097,118 + Psyr_3434 0.13 -1.1 4,097,119 - Psyr_3434 0.13 +0.2 4,097,119 - Psyr_3434 0.13 +0.5 4,097,302 + Psyr_3434 0.21 -0.6 4,097,302 + Psyr_3434 0.21 +2.1 4,097,302 + Psyr_3434 0.21 +0.5 4,097,302 + Psyr_3434 0.21 -0.1 4,097,303 - Psyr_3434 0.21 -1.9 4,097,303 - Psyr_3434 0.21 +1.4 4,097,306 + Psyr_3434 0.21 +1.0 4,097,426 - Psyr_3434 0.26 +1.5 4,097,426 - Psyr_3434 0.26 -0.5 4,097,426 - Psyr_3434 0.26 +0.7 4,097,648 + Psyr_3434 0.36 +0.1
Or see this region's nucleotide sequence