Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1425 and Psyr_1426 are separated by 655 nucleotides Psyr_1426 and Psyr_1427 overlap by 4 nucleotides Psyr_1427 and Psyr_1428 are separated by 2 nucleotides
Psyr_1425: Psyr_1425 - DNA-directed DNA polymerase, at 1,612,927 to 1,614,207
_1425
Psyr_1426: Psyr_1426 - conserved hypothetical protein, at 1,614,863 to 1,616,401
_1426
Psyr_1427: Psyr_1427 - conserved hypothetical protein, at 1,616,398 to 1,616,772
_1427
Psyr_1428: Psyr_1428 - conserved hypothetical protein, at 1,616,775 to 1,618,142
_1428
Position (kb)
1614
1615
1616
1617 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1613.953 kb on + strand, within Psyr_1425 at 1613.953 kb on + strand, within Psyr_1425 at 1613.953 kb on + strand, within Psyr_1425 at 1613.954 kb on - strand, within Psyr_1425 at 1613.954 kb on - strand, within Psyr_1425 at 1613.954 kb on - strand, within Psyr_1425 at 1614.003 kb on + strand, within Psyr_1425 at 1614.003 kb on + strand, within Psyr_1425 at 1614.004 kb on - strand, within Psyr_1425 at 1614.004 kb on - strand, within Psyr_1425 at 1614.004 kb on - strand, within Psyr_1425 at 1614.075 kb on + strand, within Psyr_1425 at 1614.075 kb on + strand, within Psyr_1425 at 1614.075 kb on + strand, within Psyr_1425 at 1614.076 kb on - strand, within Psyr_1425 at 1614.163 kb on + strand at 1614.164 kb on - strand at 1614.165 kb on + strand at 1614.165 kb on + strand at 1614.166 kb on - strand at 1614.166 kb on - strand at 1614.216 kb on + strand at 1614.217 kb on - strand at 1614.240 kb on + strand at 1614.241 kb on - strand at 1614.241 kb on - strand at 1614.245 kb on + strand at 1614.245 kb on + strand at 1614.246 kb on - strand at 1614.246 kb on - strand at 1614.246 kb on - strand at 1614.246 kb on - strand at 1614.246 kb on - strand at 1614.246 kb on - strand at 1614.303 kb on + strand at 1614.344 kb on + strand at 1614.370 kb on + strand at 1614.370 kb on + strand at 1614.370 kb on + strand at 1614.370 kb on + strand at 1614.371 kb on - strand at 1614.371 kb on - strand at 1614.374 kb on + strand at 1614.374 kb on + strand at 1614.374 kb on + strand at 1614.374 kb on + strand at 1614.375 kb on - strand at 1614.375 kb on - strand at 1614.375 kb on - strand at 1614.382 kb on + strand at 1614.382 kb on + strand at 1614.383 kb on - strand at 1614.407 kb on + strand at 1614.407 kb on + strand at 1614.407 kb on + strand at 1614.408 kb on - strand at 1614.408 kb on - strand at 1614.408 kb on - strand at 1614.475 kb on - strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.526 kb on + strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.527 kb on - strand at 1614.528 kb on + strand at 1614.528 kb on + strand at 1614.528 kb on + strand at 1614.618 kb on + strand at 1614.619 kb on - strand at 1614.700 kb on + strand at 1614.709 kb on + strand at 1614.709 kb on + strand at 1614.710 kb on - strand at 1614.710 kb on - strand at 1614.745 kb on + strand at 1614.746 kb on - strand at 1614.767 kb on + strand at 1614.783 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.785 kb on + strand at 1614.786 kb on - strand at 1614.786 kb on - strand at 1614.786 kb on - strand at 1614.789 kb on + strand at 1614.790 kb on - strand at 1614.893 kb on + strand at 1614.893 kb on + strand at 1614.893 kb on + strand at 1614.894 kb on - strand at 1614.894 kb on - strand at 1614.908 kb on - strand at 1614.923 kb on - strand at 1614.944 kb on - strand at 1614.944 kb on - strand at 1615.016 kb on + strand at 1615.017 kb on - strand, within Psyr_1426 at 1615.019 kb on - strand, within Psyr_1426 at 1615.049 kb on + strand, within Psyr_1426 at 1615.049 kb on + strand, within Psyr_1426 at 1615.049 kb on + strand, within Psyr_1426 at 1615.128 kb on + strand, within Psyr_1426 at 1615.128 kb on + strand, within Psyr_1426 at 1615.150 kb on + strand, within Psyr_1426 at 1615.150 kb on + strand, within Psyr_1426 at 1615.154 kb on + strand, within Psyr_1426 at 1615.213 kb on + strand, within Psyr_1426 at 1615.213 kb on + strand, within Psyr_1426 at 1615.213 kb on + strand, within Psyr_1426 at 1615.214 kb on - strand, within Psyr_1426 at 1615.214 kb on - strand, within Psyr_1426 at 1615.214 kb on - strand, within Psyr_1426 at 1615.257 kb on - strand, within Psyr_1426 at 1615.289 kb on + strand, within Psyr_1426 at 1615.290 kb on - strand, within Psyr_1426 at 1615.341 kb on + strand, within Psyr_1426 at 1615.341 kb on + strand, within Psyr_1426 at 1615.341 kb on + strand, within Psyr_1426 at 1615.342 kb on - strand, within Psyr_1426 at 1615.375 kb on + strand, within Psyr_1426 at 1615.375 kb on + strand, within Psyr_1426 at 1615.376 kb on - strand, within Psyr_1426 at 1615.376 kb on - strand, within Psyr_1426 at 1615.401 kb on - strand, within Psyr_1426 at 1615.429 kb on - strand, within Psyr_1426 at 1615.438 kb on - strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.624 kb on + strand, within Psyr_1426 at 1615.625 kb on - strand, within Psyr_1426 at 1615.625 kb on - strand, within Psyr_1426 at 1615.625 kb on - strand, within Psyr_1426 at 1615.734 kb on + strand, within Psyr_1426 at 1615.734 kb on + strand, within Psyr_1426 at 1615.735 kb on - strand, within Psyr_1426 at 1615.846 kb on - strand, within Psyr_1426 at 1615.952 kb on - strand, within Psyr_1426 at 1615.952 kb on - strand, within Psyr_1426 at 1615.952 kb on - strand, within Psyr_1426 at 1615.952 kb on - strand, within Psyr_1426 at 1616.029 kb on + strand, within Psyr_1426 at 1616.030 kb on - strand, within Psyr_1426 at 1616.030 kb on - strand, within Psyr_1426 at 1616.066 kb on - strand, within Psyr_1426 at 1616.088 kb on + strand, within Psyr_1426 at 1616.088 kb on + strand, within Psyr_1426 at 1616.129 kb on - strand, within Psyr_1426 at 1616.144 kb on + strand, within Psyr_1426 at 1616.174 kb on + strand, within Psyr_1426 at 1616.174 kb on + strand, within Psyr_1426 at 1616.175 kb on - strand, within Psyr_1426 at 1616.175 kb on - strand, within Psyr_1426 at 1616.187 kb on + strand, within Psyr_1426 at 1616.187 kb on + strand, within Psyr_1426 at 1616.187 kb on + strand, within Psyr_1426 at 1616.188 kb on - strand, within Psyr_1426 at 1616.188 kb on - strand, within Psyr_1426 at 1616.201 kb on + strand, within Psyr_1426 at 1616.342 kb on + strand at 1616.343 kb on - strand at 1616.367 kb on + strand at 1616.367 kb on + strand at 1616.370 kb on + strand at 1616.371 kb on - strand at 1616.371 kb on - strand at 1616.371 kb on - strand at 1616.380 kb on - strand at 1616.380 kb on - strand at 1616.380 kb on - strand at 1616.380 kb on - strand at 1616.420 kb on - strand at 1616.501 kb on - strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.597 kb on + strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.598 kb on - strand, within Psyr_1427 at 1616.603 kb on + strand, within Psyr_1427 at 1616.603 kb on + strand, within Psyr_1427 at 1616.603 kb on + strand, within Psyr_1427 at 1616.637 kb on + strand, within Psyr_1427 at 1616.653 kb on + strand, within Psyr_1427 at 1616.653 kb on + strand, within Psyr_1427 at 1616.653 kb on + strand, within Psyr_1427 at 1616.654 kb on - strand, within Psyr_1427 at 1616.654 kb on - strand, within Psyr_1427 at 1616.654 kb on - strand, within Psyr_1427 at 1616.654 kb on - strand, within Psyr_1427 at 1616.716 kb on + strand, within Psyr_1427 at 1616.716 kb on + strand, within Psyr_1427 at 1616.717 kb on - strand, within Psyr_1427 at 1616.717 kb on - strand, within Psyr_1427 at 1616.737 kb on - strand at 1616.740 kb on + strand at 1616.741 kb on - strand at 1616.780 kb on + strand at 1616.781 kb on - strand at 1617.052 kb on - strand, within Psyr_1428 at 1617.254 kb on - strand, within Psyr_1428 at 1617.254 kb on - strand, within Psyr_1428 at 1617.254 kb on - strand, within Psyr_1428 at 1617.365 kb on + strand, within Psyr_1428
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 1,613,953 + Psyr_1425 0.80 +1.0 1,613,953 + Psyr_1425 0.80 +0.3 1,613,953 + Psyr_1425 0.80 +1.4 1,613,954 - Psyr_1425 0.80 +3.1 1,613,954 - Psyr_1425 0.80 +0.4 1,613,954 - Psyr_1425 0.80 -0.0 1,614,003 + Psyr_1425 0.84 +0.8 1,614,003 + Psyr_1425 0.84 +0.6 1,614,004 - Psyr_1425 0.84 -0.1 1,614,004 - Psyr_1425 0.84 -1.7 1,614,004 - Psyr_1425 0.84 +1.1 1,614,075 + Psyr_1425 0.90 +0.7 1,614,075 + Psyr_1425 0.90 -0.3 1,614,075 + Psyr_1425 0.90 +1.0 1,614,076 - Psyr_1425 0.90 -0.5 1,614,163 + +0.5 1,614,164 - -0.2 1,614,165 + +0.2 1,614,165 + -0.3 1,614,166 - +0.0 1,614,166 - +1.6 1,614,216 + -1.0 1,614,217 - +0.9 1,614,240 + -0.3 1,614,241 - -1.3 1,614,241 - +2.2 1,614,245 + -2.4 1,614,245 + +0.4 1,614,246 - -0.4 1,614,246 - +0.5 1,614,246 - -2.3 1,614,246 - -1.5 1,614,246 - -2.0 1,614,246 - -0.9 1,614,303 + -0.8 1,614,344 + +2.2 1,614,370 + +0.9 1,614,370 + -0.6 1,614,370 + -0.8 1,614,370 + -0.3 1,614,371 - -3.6 1,614,371 - +1.7 1,614,374 + -0.9 1,614,374 + +0.3 1,614,374 + -0.8 1,614,374 + -0.3 1,614,375 - -0.9 1,614,375 - +0.1 1,614,375 - -0.9 1,614,382 + -0.5 1,614,382 + +0.7 1,614,383 - -1.5 1,614,407 + -1.3 1,614,407 + -1.3 1,614,407 + -2.7 1,614,408 - +0.7 1,614,408 - -0.9 1,614,408 - -2.5 1,614,475 - -2.5 1,614,526 + -0.1 1,614,526 + +0.8 1,614,526 + -1.1 1,614,526 + +2.7 1,614,526 + -0.3 1,614,526 + -0.8 1,614,526 + -0.3 1,614,526 + -1.6 1,614,526 + -0.5 1,614,526 + +0.8 1,614,526 + +2.2 1,614,526 + -1.1 1,614,526 + -0.1 1,614,526 + -0.0 1,614,526 + -1.6 1,614,526 + +1.2 1,614,526 + +1.2 1,614,526 + -1.6 1,614,526 + +0.5 1,614,526 + +0.0 1,614,526 + +0.7 1,614,527 - -1.4 1,614,527 - -0.0 1,614,527 - -3.9 1,614,527 - +1.2 1,614,527 - -0.1 1,614,527 - -0.1 1,614,527 - -1.5 1,614,527 - -0.6 1,614,527 - +0.2 1,614,527 - -0.8 1,614,527 - -1.1 1,614,527 - -0.2 1,614,527 - -0.9 1,614,527 - -0.8 1,614,527 - +0.2 1,614,528 + -0.3 1,614,528 + +1.8 1,614,528 + +1.2 1,614,618 + +0.8 1,614,619 - +1.4 1,614,700 + +0.0 1,614,709 + +1.8 1,614,709 + -0.7 1,614,710 - -1.3 1,614,710 - +0.6 1,614,745 + +0.3 1,614,746 - +0.9 1,614,767 + -3.1 1,614,783 + -0.0 1,614,785 + -1.0 1,614,785 + -0.4 1,614,785 + +1.4 1,614,785 + -2.6 1,614,785 + -0.8 1,614,785 + -0.0 1,614,785 + +0.7 1,614,786 - -2.8 1,614,786 - -0.3 1,614,786 - +1.4 1,614,789 + -0.4 1,614,790 - +0.9 1,614,893 + +0.0 1,614,893 + -0.3 1,614,893 + -0.0 1,614,894 - -0.9 1,614,894 - -3.7 1,614,908 - +1.5 1,614,923 - -0.1 1,614,944 - -0.1 1,614,944 - +1.2 1,615,016 + -0.6 1,615,017 - Psyr_1426 0.10 +0.7 1,615,019 - Psyr_1426 0.10 +0.4 1,615,049 + Psyr_1426 0.12 +0.2 1,615,049 + Psyr_1426 0.12 +0.2 1,615,049 + Psyr_1426 0.12 -0.3 1,615,128 + Psyr_1426 0.17 -0.0 1,615,128 + Psyr_1426 0.17 +4.8 1,615,150 + Psyr_1426 0.19 +1.2 1,615,150 + Psyr_1426 0.19 +0.6 1,615,154 + Psyr_1426 0.19 -2.3 1,615,213 + Psyr_1426 0.23 +0.1 1,615,213 + Psyr_1426 0.23 +1.0 1,615,213 + Psyr_1426 0.23 +0.7 1,615,214 - Psyr_1426 0.23 -1.2 1,615,214 - Psyr_1426 0.23 -0.1 1,615,214 - Psyr_1426 0.23 +0.2 1,615,257 - Psyr_1426 0.26 +0.0 1,615,289 + Psyr_1426 0.28 +1.8 1,615,290 - Psyr_1426 0.28 -1.0 1,615,341 + Psyr_1426 0.31 +0.1 1,615,341 + Psyr_1426 0.31 -2.2 1,615,341 + Psyr_1426 0.31 -0.4 1,615,342 - Psyr_1426 0.31 +0.7 1,615,375 + Psyr_1426 0.33 -0.3 1,615,375 + Psyr_1426 0.33 -0.7 1,615,376 - Psyr_1426 0.33 -0.0 1,615,376 - Psyr_1426 0.33 +0.7 1,615,401 - Psyr_1426 0.35 +1.0 1,615,429 - Psyr_1426 0.37 +0.4 1,615,438 - Psyr_1426 0.37 -0.2 1,615,624 + Psyr_1426 0.49 -0.3 1,615,624 + Psyr_1426 0.49 +0.4 1,615,624 + Psyr_1426 0.49 +0.2 1,615,624 + Psyr_1426 0.49 -0.9 1,615,624 + Psyr_1426 0.49 -0.7 1,615,624 + Psyr_1426 0.49 +0.5 1,615,625 - Psyr_1426 0.50 +1.1 1,615,625 - Psyr_1426 0.50 -2.0 1,615,625 - Psyr_1426 0.50 +1.5 1,615,734 + Psyr_1426 0.57 -1.2 1,615,734 + Psyr_1426 0.57 -0.6 1,615,735 - Psyr_1426 0.57 -0.7 1,615,846 - Psyr_1426 0.64 +0.5 1,615,952 - Psyr_1426 0.71 -0.6 1,615,952 - Psyr_1426 0.71 +0.4 1,615,952 - Psyr_1426 0.71 +0.6 1,615,952 - Psyr_1426 0.71 +0.9 1,616,029 + Psyr_1426 0.76 +0.8 1,616,030 - Psyr_1426 0.76 +0.4 1,616,030 - Psyr_1426 0.76 +3.2 1,616,066 - Psyr_1426 0.78 +3.2 1,616,088 + Psyr_1426 0.80 +4.4 1,616,088 + Psyr_1426 0.80 -0.7 1,616,129 - Psyr_1426 0.82 -0.3 1,616,144 + Psyr_1426 0.83 +0.4 1,616,174 + Psyr_1426 0.85 -0.4 1,616,174 + Psyr_1426 0.85 +0.7 1,616,175 - Psyr_1426 0.85 -2.1 1,616,175 - Psyr_1426 0.85 -1.1 1,616,187 + Psyr_1426 0.86 +0.1 1,616,187 + Psyr_1426 0.86 +0.1 1,616,187 + Psyr_1426 0.86 +0.8 1,616,188 - Psyr_1426 0.86 -1.7 1,616,188 - Psyr_1426 0.86 -0.7 1,616,201 + Psyr_1426 0.87 -0.7 1,616,342 + +0.1 1,616,343 - -4.4 1,616,367 + +0.5 1,616,367 + +2.8 1,616,370 + -0.9 1,616,371 - +0.6 1,616,371 - +0.7 1,616,371 - +1.4 1,616,380 - -0.3 1,616,380 - -0.2 1,616,380 - -0.5 1,616,380 - -0.7 1,616,420 - -0.8 1,616,501 - Psyr_1427 0.27 -1.1 1,616,597 + Psyr_1427 0.53 +1.4 1,616,597 + Psyr_1427 0.53 +2.0 1,616,597 + Psyr_1427 0.53 +3.3 1,616,597 + Psyr_1427 0.53 +1.3 1,616,597 + Psyr_1427 0.53 +1.3 1,616,597 + Psyr_1427 0.53 -2.5 1,616,598 - Psyr_1427 0.53 +0.1 1,616,598 - Psyr_1427 0.53 -1.3 1,616,598 - Psyr_1427 0.53 +0.5 1,616,598 - Psyr_1427 0.53 +0.1 1,616,598 - Psyr_1427 0.53 -0.2 1,616,598 - Psyr_1427 0.53 +2.0 1,616,598 - Psyr_1427 0.53 +1.7 1,616,598 - Psyr_1427 0.53 +0.3 1,616,598 - Psyr_1427 0.53 -0.1 1,616,598 - Psyr_1427 0.53 -0.9 1,616,598 - Psyr_1427 0.53 +2.3 1,616,603 + Psyr_1427 0.55 -0.1 1,616,603 + Psyr_1427 0.55 +0.8 1,616,603 + Psyr_1427 0.55 +0.9 1,616,637 + Psyr_1427 0.64 +0.4 1,616,653 + Psyr_1427 0.68 -0.7 1,616,653 + Psyr_1427 0.68 -1.1 1,616,653 + Psyr_1427 0.68 -0.5 1,616,654 - Psyr_1427 0.68 -0.2 1,616,654 - Psyr_1427 0.68 -0.9 1,616,654 - Psyr_1427 0.68 -0.5 1,616,654 - Psyr_1427 0.68 +1.0 1,616,716 + Psyr_1427 0.85 +0.4 1,616,716 + Psyr_1427 0.85 +0.5 1,616,717 - Psyr_1427 0.85 +0.3 1,616,717 - Psyr_1427 0.85 -1.4 1,616,737 - +4.4 1,616,740 + +0.3 1,616,741 - -0.1 1,616,780 + +0.4 1,616,781 - -0.8 1,617,052 - Psyr_1428 0.20 -0.0 1,617,254 - Psyr_1428 0.35 +1.0 1,617,254 - Psyr_1428 0.35 +0.2 1,617,254 - Psyr_1428 0.35 +0.1 1,617,365 + Psyr_1428 0.43 +0.1
Or see this region's nucleotide sequence