Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1064 and Psyr_1065 are separated by 202 nucleotides Psyr_1065 and Psyr_1066 are separated by 294 nucleotides
Psyr_1064: Psyr_1064 - Protein of unknown function DUF328, at 1,206,856 to 1,207,635
_1064
Psyr_1065: Psyr_1065 - Polysaccharide deacetylase, at 1,207,838 to 1,209,016
_1065
Psyr_1066: Psyr_1066 - PhoH-like protein, at 1,209,311 to 1,210,705
_1066
Position (kb)
1207
1208
1209
1210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1206.857 kb on + strand at 1206.857 kb on + strand at 1206.858 kb on - strand at 1206.931 kb on + strand at 1206.932 kb on - strand at 1206.932 kb on - strand at 1206.932 kb on - strand at 1206.946 kb on + strand, within Psyr_1064 at 1206.947 kb on - strand, within Psyr_1064 at 1206.947 kb on - strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1207.017 kb on - strand, within Psyr_1064 at 1207.017 kb on - strand, within Psyr_1064 at 1207.034 kb on + strand, within Psyr_1064 at 1207.035 kb on - strand, within Psyr_1064 at 1207.113 kb on - strand, within Psyr_1064 at 1207.113 kb on - strand, within Psyr_1064 at 1207.113 kb on - strand, within Psyr_1064 at 1207.373 kb on + strand, within Psyr_1064 at 1207.373 kb on + strand, within Psyr_1064 at 1207.373 kb on + strand, within Psyr_1064 at 1207.373 kb on + strand, within Psyr_1064 at 1207.694 kb on + strand at 1207.694 kb on + strand at 1207.694 kb on + strand at 1207.695 kb on - strand at 1207.695 kb on - strand at 1207.695 kb on - strand at 1207.695 kb on - strand at 1207.695 kb on - strand at 1207.749 kb on + strand at 1207.749 kb on + strand at 1207.749 kb on + strand at 1207.750 kb on - strand at 1207.763 kb on + strand at 1207.763 kb on + strand at 1207.763 kb on + strand at 1207.763 kb on + strand at 1207.763 kb on + strand at 1207.764 kb on - strand at 1207.764 kb on - strand at 1207.764 kb on - strand at 1207.779 kb on + strand at 1207.779 kb on + strand at 1207.779 kb on + strand at 1207.779 kb on + strand at 1207.779 kb on + strand at 1207.779 kb on + strand at 1207.780 kb on - strand at 1207.780 kb on - strand at 1207.780 kb on - strand at 1207.780 kb on - strand at 1207.780 kb on - strand at 1207.780 kb on - strand at 1207.786 kb on + strand at 1207.786 kb on + strand at 1207.786 kb on + strand at 1207.786 kb on + strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.787 kb on - strand at 1207.852 kb on - strand at 1208.006 kb on + strand, within Psyr_1065 at 1208.006 kb on + strand, within Psyr_1065 at 1208.006 kb on + strand, within Psyr_1065 at 1208.007 kb on - strand, within Psyr_1065 at 1208.123 kb on + strand, within Psyr_1065 at 1208.123 kb on + strand, within Psyr_1065 at 1208.124 kb on - strand, within Psyr_1065 at 1208.124 kb on - strand, within Psyr_1065 at 1208.124 kb on - strand, within Psyr_1065 at 1208.213 kb on + strand, within Psyr_1065 at 1208.214 kb on - strand, within Psyr_1065 at 1208.237 kb on + strand, within Psyr_1065 at 1208.237 kb on + strand, within Psyr_1065 at 1208.237 kb on + strand, within Psyr_1065 at 1208.237 kb on + strand, within Psyr_1065 at 1208.237 kb on + strand, within Psyr_1065 at 1208.238 kb on - strand, within Psyr_1065 at 1208.238 kb on - strand, within Psyr_1065 at 1208.342 kb on + strand, within Psyr_1065 at 1208.343 kb on - strand, within Psyr_1065 at 1208.694 kb on - strand, within Psyr_1065 at 1208.950 kb on + strand at 1208.950 kb on + strand at 1208.950 kb on + strand at 1209.049 kb on + strand at 1209.079 kb on - strand at 1209.147 kb on + strand at 1209.147 kb on + strand at 1209.147 kb on + strand at 1209.147 kb on + strand at 1209.148 kb on - strand at 1209.148 kb on - strand at 1209.148 kb on - strand at 1209.148 kb on - strand at 1209.148 kb on - strand at 1209.150 kb on + strand at 1209.150 kb on + strand at 1209.294 kb on + strand at 1209.294 kb on + strand at 1209.294 kb on + strand at 1209.295 kb on - strand at 1209.295 kb on - strand at 1209.356 kb on + strand at 1209.357 kb on - strand at 1209.357 kb on - strand at 1209.376 kb on - strand at 1209.581 kb on - strand, within Psyr_1066 at 1209.600 kb on - strand, within Psyr_1066 at 1209.789 kb on - strand, within Psyr_1066 at 1209.950 kb on - strand, within Psyr_1066 at 1209.985 kb on - strand, within Psyr_1066
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 1,206,857 + -0.6 1,206,857 + -1.3 1,206,858 - +1.0 1,206,931 + -0.6 1,206,932 - -0.7 1,206,932 - +0.3 1,206,932 - +0.7 1,206,946 + Psyr_1064 0.12 -0.1 1,206,947 - Psyr_1064 0.12 -0.0 1,206,947 - Psyr_1064 0.12 -0.1 1,206,976 + Psyr_1064 0.15 +3.2 1,206,976 + Psyr_1064 0.15 +1.2 1,206,976 + Psyr_1064 0.15 +0.9 1,206,976 + Psyr_1064 0.15 +0.5 1,206,977 - Psyr_1064 0.16 -1.2 1,206,977 - Psyr_1064 0.16 -0.3 1,206,977 - Psyr_1064 0.16 -0.6 1,207,017 - Psyr_1064 0.21 +0.7 1,207,017 - Psyr_1064 0.21 -0.1 1,207,034 + Psyr_1064 0.23 -0.1 1,207,035 - Psyr_1064 0.23 -0.5 1,207,113 - Psyr_1064 0.33 +2.2 1,207,113 - Psyr_1064 0.33 +0.1 1,207,113 - Psyr_1064 0.33 -0.4 1,207,373 + Psyr_1064 0.66 +0.4 1,207,373 + Psyr_1064 0.66 +0.5 1,207,373 + Psyr_1064 0.66 +0.2 1,207,373 + Psyr_1064 0.66 -0.4 1,207,694 + -0.0 1,207,694 + +0.0 1,207,694 + -1.3 1,207,695 - -0.6 1,207,695 - +0.2 1,207,695 - +0.6 1,207,695 - +1.0 1,207,695 - -0.0 1,207,749 + +0.6 1,207,749 + +0.4 1,207,749 + -0.4 1,207,750 - +0.3 1,207,763 + -1.2 1,207,763 + +0.2 1,207,763 + +0.7 1,207,763 + -3.6 1,207,763 + -0.8 1,207,764 - +0.8 1,207,764 - +0.2 1,207,764 - +0.1 1,207,779 + +0.6 1,207,779 + +0.6 1,207,779 + +0.7 1,207,779 + -0.0 1,207,779 + -0.1 1,207,779 + +2.2 1,207,780 - +0.1 1,207,780 - -1.0 1,207,780 - +1.7 1,207,780 - -0.2 1,207,780 - +0.5 1,207,780 - +1.6 1,207,786 + -0.3 1,207,786 + +3.8 1,207,786 + +1.2 1,207,786 + -0.5 1,207,787 - +0.3 1,207,787 - -1.4 1,207,787 - -2.3 1,207,787 - -0.6 1,207,787 - +0.1 1,207,787 - -0.3 1,207,787 - -0.9 1,207,852 - +0.3 1,208,006 + Psyr_1065 0.14 -0.8 1,208,006 + Psyr_1065 0.14 -1.0 1,208,006 + Psyr_1065 0.14 +0.2 1,208,007 - Psyr_1065 0.14 -2.6 1,208,123 + Psyr_1065 0.24 -0.4 1,208,123 + Psyr_1065 0.24 +0.9 1,208,124 - Psyr_1065 0.24 +0.1 1,208,124 - Psyr_1065 0.24 +2.4 1,208,124 - Psyr_1065 0.24 -0.7 1,208,213 + Psyr_1065 0.32 -0.4 1,208,214 - Psyr_1065 0.32 -0.1 1,208,237 + Psyr_1065 0.34 -0.5 1,208,237 + Psyr_1065 0.34 -0.5 1,208,237 + Psyr_1065 0.34 +0.2 1,208,237 + Psyr_1065 0.34 -0.3 1,208,237 + Psyr_1065 0.34 -0.2 1,208,238 - Psyr_1065 0.34 +0.6 1,208,238 - Psyr_1065 0.34 +0.1 1,208,342 + Psyr_1065 0.43 -1.1 1,208,343 - Psyr_1065 0.43 -1.0 1,208,694 - Psyr_1065 0.73 +1.1 1,208,950 + -1.6 1,208,950 + -3.1 1,208,950 + -0.3 1,209,049 + +1.4 1,209,079 - -0.3 1,209,147 + +0.8 1,209,147 + -0.2 1,209,147 + +0.1 1,209,147 + +1.5 1,209,148 - -0.4 1,209,148 - +3.5 1,209,148 - +0.7 1,209,148 - -0.6 1,209,148 - +1.2 1,209,150 + +1.8 1,209,150 + -0.4 1,209,294 + -0.0 1,209,294 + +0.9 1,209,294 + -1.1 1,209,295 - +4.0 1,209,295 - -0.6 1,209,356 + -0.5 1,209,357 - -0.3 1,209,357 - +0.7 1,209,376 - -0.1 1,209,581 - Psyr_1066 0.19 -0.2 1,209,600 - Psyr_1066 0.21 +1.1 1,209,789 - Psyr_1066 0.34 +0.7 1,209,950 - Psyr_1066 0.46 -0.7 1,209,985 - Psyr_1066 0.48 -0.9
Or see this region's nucleotide sequence