Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0266 and Psyr_0267 overlap by 4 nucleotides Psyr_0267 and Psyr_0268 are separated by 9 nucleotides Psyr_0268 and Psyr_0269 are separated by 221 nucleotides
Psyr_0266: Psyr_0266 - GGDEF domain protein, at 282,921 to 284,993
_0266
Psyr_0267: Psyr_0267 - Endonuclease/exonuclease/phosphatase, at 284,990 to 285,865
_0267
Psyr_0268: Psyr_0268 - DSBA oxidoreductase, at 285,875 to 286,519
_0268
Psyr_0269: Psyr_0269 - cell division checkpoint GTPase YihA, at 286,741 to 287,448
_0269
Position (kb) 284
285
286 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 284.362 kb on + strand, within Psyr_0266 at 284.362 kb on + strand, within Psyr_0266 at 284.362 kb on + strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.647 kb on - strand, within Psyr_0266 at 284.647 kb on - strand, within Psyr_0266 at 284.959 kb on + strand at 284.959 kb on + strand at 284.959 kb on + strand at 284.960 kb on - strand at 284.960 kb on - strand at 284.960 kb on - strand at 284.960 kb on - strand at 285.098 kb on + strand, within Psyr_0267 at 285.185 kb on + strand, within Psyr_0267 at 285.186 kb on - strand, within Psyr_0267 at 285.186 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.467 kb on + strand, within Psyr_0267 at 285.705 kb on + strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand at 286.039 kb on - strand, within Psyr_0268 at 286.277 kb on + strand, within Psyr_0268 at 286.356 kb on + strand, within Psyr_0268 at 286.426 kb on - strand, within Psyr_0268 at 286.426 kb on - strand, within Psyr_0268 at 286.514 kb on - strand at 286.524 kb on + strand at 286.524 kb on + strand at 286.525 kb on - strand at 286.525 kb on - strand at 286.525 kb on - strand at 286.597 kb on + strand at 286.597 kb on + strand at 286.611 kb on + strand at 286.611 kb on + strand at 286.645 kb on - strand at 286.645 kb on - strand at 286.645 kb on - strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 284,362 + Psyr_0266 0.70 +0.4 284,362 + Psyr_0266 0.70 +0.5 284,362 + Psyr_0266 0.70 +3.0 284,363 - Psyr_0266 0.70 -0.0 284,363 - Psyr_0266 0.70 +0.5 284,363 - Psyr_0266 0.70 +0.8 284,363 - Psyr_0266 0.70 +0.6 284,363 - Psyr_0266 0.70 -0.1 284,367 - Psyr_0266 0.70 -0.4 284,367 - Psyr_0266 0.70 -0.6 284,367 - Psyr_0266 0.70 -1.4 284,646 + Psyr_0266 0.83 -0.8 284,646 + Psyr_0266 0.83 -0.2 284,646 + Psyr_0266 0.83 -0.1 284,646 + Psyr_0266 0.83 +1.1 284,647 - Psyr_0266 0.83 +0.9 284,647 - Psyr_0266 0.83 -0.1 284,959 + -1.1 284,959 + +0.1 284,959 + +1.0 284,960 - +0.2 284,960 - +1.0 284,960 - -0.2 284,960 - -0.5 285,098 + Psyr_0267 0.12 +0.8 285,185 + Psyr_0267 0.22 -0.6 285,186 - Psyr_0267 0.22 -0.8 285,186 - Psyr_0267 0.22 -1.7 285,307 - Psyr_0267 0.36 +0.3 285,307 - Psyr_0267 0.36 -1.7 285,307 - Psyr_0267 0.36 -0.7 285,467 + Psyr_0267 0.54 -1.2 285,705 + Psyr_0267 0.82 -0.4 285,706 - Psyr_0267 0.82 -1.6 285,706 - Psyr_0267 0.82 +2.2 285,706 - Psyr_0267 0.82 +0.0 285,876 + -0.5 285,876 + +0.1 285,876 + -1.5 285,876 + -0.2 285,876 + -0.3 286,039 - Psyr_0268 0.25 -2.0 286,277 + Psyr_0268 0.62 -1.7 286,356 + Psyr_0268 0.75 -1.7 286,426 - Psyr_0268 0.85 +2.2 286,426 - Psyr_0268 0.85 +1.4 286,514 - +1.4 286,524 + +3.0 286,524 + +1.4 286,525 - +0.2 286,525 - -0.2 286,525 - +1.4 286,597 + +1.0 286,597 + +0.4 286,611 + +0.1 286,611 + +1.3 286,645 - -0.9 286,645 - +0.4 286,645 - -0.0 286,738 + -2.3 286,738 + -1.6 286,738 + +2.4 286,738 + -0.9 286,738 + -0.6 286,738 + +0.4 286,738 + +0.1 286,740 + -1.4 286,740 + +1.1 286,740 + +0.3 286,740 + +0.5 286,740 + +0.0 286,740 + -0.3 286,740 + +0.3 286,740 + -0.2
Or see this region's nucleotide sequence