Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0221 and Psyr_0222 are separated by 96 nucleotides Psyr_0222 and Psyr_0223 are separated by 133 nucleotides Psyr_0223 and Psyr_0224 are separated by 364 nucleotides Psyr_0224 and Psyr_0225 are separated by 11 nucleotides Psyr_0225 and Psyr_0226 are separated by 81 nucleotides
Psyr_0221: Psyr_0221 - Phosphopantothenate-cysteine ligase / Phosphopantothenoylcysteine decarboxylase, at 235,108 to 236,355
_0221
Psyr_0222: Psyr_0222 - DNA replication and repair protein RadC, at 236,452 to 237,126
_0222
Psyr_0223: Psyr_0223 - extracellular solute-binding protein, family 5, at 237,260 to 238,846
_0223
Psyr_0224: Psyr_0224 - LSU ribosomal protein L28P, at 239,211 to 239,444
_0224
Psyr_0225: Psyr_0225 - LSU ribosomal protein L33P, at 239,456 to 239,611
_0225
Psyr_0226: Psyr_0226 - Protein of unknown function DUF861, at 239,693 to 240,061
_0226
Position (kb) 237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 236.403 kb on - strand at 236.451 kb on + strand at 236.451 kb on + strand at 236.452 kb on - strand at 236.458 kb on - strand at 236.458 kb on - strand at 236.458 kb on - strand at 236.458 kb on - strand at 236.545 kb on - strand, within Psyr_0222 at 236.582 kb on + strand, within Psyr_0222 at 236.582 kb on + strand, within Psyr_0222 at 236.583 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.786 kb on - strand, within Psyr_0222 at 236.873 kb on + strand, within Psyr_0222 at 236.873 kb on + strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.874 kb on - strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.902 kb on + strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.903 kb on - strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on + strand, within Psyr_0222 at 236.906 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 236.907 kb on - strand, within Psyr_0222 at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.124 kb on + strand at 237.125 kb on - strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.261 kb on + strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.262 kb on - strand at 237.280 kb on - strand at 237.312 kb on + strand at 237.408 kb on + strand at 237.408 kb on + strand at 237.409 kb on - strand at 237.409 kb on - strand at 237.409 kb on - strand at 237.446 kb on - strand, within Psyr_0223 at 237.742 kb on + strand, within Psyr_0223 at 237.743 kb on - strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.903 kb on + strand, within Psyr_0223 at 237.904 kb on - strand, within Psyr_0223 at 237.904 kb on - strand, within Psyr_0223 at 238.076 kb on + strand, within Psyr_0223 at 238.137 kb on + strand, within Psyr_0223 at 238.137 kb on + strand, within Psyr_0223 at 238.173 kb on + strand, within Psyr_0223 at 238.174 kb on - strand, within Psyr_0223 at 238.191 kb on + strand, within Psyr_0223 at 238.192 kb on - strand, within Psyr_0223 at 238.297 kb on + strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.298 kb on - strand, within Psyr_0223 at 238.300 kb on - strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.311 kb on + strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.312 kb on - strand, within Psyr_0223 at 238.551 kb on + strand, within Psyr_0223 at 238.552 kb on - strand, within Psyr_0223 at 238.552 kb on - strand, within Psyr_0223 at 238.596 kb on + strand, within Psyr_0223 at 238.597 kb on - strand, within Psyr_0223 at 238.597 kb on - strand, within Psyr_0223 at 238.597 kb on - strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.628 kb on - strand, within Psyr_0223 at 238.673 kb on + strand, within Psyr_0223 at 238.674 kb on - strand, within Psyr_0223 at 238.674 kb on - strand, within Psyr_0223 at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.728 kb on + strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.729 kb on - strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.733 kb on + strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.734 kb on - strand at 238.852 kb on + strand at 238.909 kb on + strand at 238.910 kb on - strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.918 kb on + strand at 238.919 kb on - strand at 238.919 kb on - strand at 239.690 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.694 kb on + strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.695 kb on - strand at 239.698 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.724 kb on + strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.725 kb on - strand at 239.758 kb on + strand, within Psyr_0226 at 239.758 kb on + strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226 at 239.759 kb on - strand, within Psyr_0226
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 236,403 - +0.0 236,451 + +0.9 236,451 + +0.2 236,452 - +0.9 236,458 - -0.4 236,458 - -0.1 236,458 - -0.2 236,458 - -0.6 236,545 - Psyr_0222 0.14 -0.7 236,582 + Psyr_0222 0.19 -1.7 236,582 + Psyr_0222 0.19 -0.6 236,583 - Psyr_0222 0.19 -1.1 236,786 - Psyr_0222 0.49 -0.0 236,786 - Psyr_0222 0.49 +0.7 236,873 + Psyr_0222 0.62 +0.4 236,873 + Psyr_0222 0.62 -0.3 236,874 - Psyr_0222 0.63 +0.2 236,874 - Psyr_0222 0.63 -1.2 236,902 + Psyr_0222 0.67 +0.9 236,902 + Psyr_0222 0.67 -0.6 236,902 + Psyr_0222 0.67 +0.1 236,902 + Psyr_0222 0.67 -1.2 236,902 + Psyr_0222 0.67 -2.0 236,902 + Psyr_0222 0.67 -1.8 236,902 + Psyr_0222 0.67 +0.0 236,902 + Psyr_0222 0.67 +0.1 236,902 + Psyr_0222 0.67 +0.1 236,902 + Psyr_0222 0.67 -0.2 236,902 + Psyr_0222 0.67 +0.0 236,902 + Psyr_0222 0.67 +1.4 236,903 - Psyr_0222 0.67 -1.7 236,903 - Psyr_0222 0.67 +0.1 236,903 - Psyr_0222 0.67 +0.4 236,903 - Psyr_0222 0.67 +4.3 236,903 - Psyr_0222 0.67 -0.1 236,903 - Psyr_0222 0.67 +1.4 236,903 - Psyr_0222 0.67 -0.4 236,903 - Psyr_0222 0.67 -3.1 236,903 - Psyr_0222 0.67 -0.9 236,903 - Psyr_0222 0.67 -1.1 236,903 - Psyr_0222 0.67 +0.0 236,903 - Psyr_0222 0.67 +0.6 236,903 - Psyr_0222 0.67 -0.3 236,906 + Psyr_0222 0.67 -0.6 236,906 + Psyr_0222 0.67 +0.3 236,906 + Psyr_0222 0.67 -2.3 236,906 + Psyr_0222 0.67 +0.9 236,906 + Psyr_0222 0.67 -0.7 236,906 + Psyr_0222 0.67 -1.1 236,906 + Psyr_0222 0.67 -0.9 236,906 + Psyr_0222 0.67 +3.4 236,906 - Psyr_0222 0.67 +0.4 236,907 - Psyr_0222 0.67 -0.9 236,907 - Psyr_0222 0.67 -1.4 236,907 - Psyr_0222 0.67 +1.4 236,907 - Psyr_0222 0.67 -1.7 236,907 - Psyr_0222 0.67 -0.8 236,907 - Psyr_0222 0.67 -0.5 236,907 - Psyr_0222 0.67 +0.8 236,907 - Psyr_0222 0.67 -1.0 236,907 - Psyr_0222 0.67 +1.1 236,907 - Psyr_0222 0.67 +4.3 236,907 - Psyr_0222 0.67 +0.2 236,907 - Psyr_0222 0.67 -0.1 236,907 - Psyr_0222 0.67 -0.0 237,124 + -0.6 237,124 + -0.6 237,124 + +0.8 237,124 + -0.3 237,125 - +1.6 237,261 + -1.1 237,261 + -0.9 237,261 + -0.3 237,261 + +1.7 237,261 + -0.8 237,261 + +0.2 237,261 + +0.6 237,262 - -0.6 237,262 - -0.6 237,262 - -0.5 237,262 - -0.6 237,280 - -1.7 237,312 + +0.4 237,408 + -0.9 237,408 + +0.9 237,409 - -1.2 237,409 - -0.9 237,409 - +0.4 237,446 - Psyr_0223 0.12 -0.1 237,742 + Psyr_0223 0.30 -1.1 237,743 - Psyr_0223 0.30 +1.2 237,903 + Psyr_0223 0.41 -0.8 237,903 + Psyr_0223 0.41 +1.1 237,903 + Psyr_0223 0.41 -0.3 237,904 - Psyr_0223 0.41 -2.2 237,904 - Psyr_0223 0.41 +1.3 238,076 + Psyr_0223 0.51 +0.3 238,137 + Psyr_0223 0.55 +0.4 238,137 + Psyr_0223 0.55 -0.2 238,173 + Psyr_0223 0.58 -0.5 238,174 - Psyr_0223 0.58 +0.3 238,191 + Psyr_0223 0.59 +0.4 238,192 - Psyr_0223 0.59 -0.1 238,297 + Psyr_0223 0.65 +0.0 238,298 - Psyr_0223 0.65 -0.9 238,298 - Psyr_0223 0.65 -0.8 238,298 - Psyr_0223 0.65 -2.0 238,298 - Psyr_0223 0.65 -2.1 238,298 - Psyr_0223 0.65 +0.0 238,298 - Psyr_0223 0.65 +0.5 238,298 - Psyr_0223 0.65 -0.1 238,300 - Psyr_0223 0.66 -0.6 238,311 + Psyr_0223 0.66 -1.1 238,311 + Psyr_0223 0.66 -0.3 238,311 + Psyr_0223 0.66 -1.5 238,311 + Psyr_0223 0.66 +1.4 238,311 + Psyr_0223 0.66 -0.3 238,311 + Psyr_0223 0.66 -1.3 238,311 + Psyr_0223 0.66 -2.1 238,311 + Psyr_0223 0.66 +0.1 238,312 - Psyr_0223 0.66 -1.0 238,312 - Psyr_0223 0.66 +0.6 238,312 - Psyr_0223 0.66 -0.6 238,312 - Psyr_0223 0.66 +0.3 238,312 - Psyr_0223 0.66 -0.7 238,312 - Psyr_0223 0.66 -0.9 238,551 + Psyr_0223 0.81 -0.0 238,552 - Psyr_0223 0.81 -1.1 238,552 - Psyr_0223 0.81 +0.1 238,596 + Psyr_0223 0.84 -0.1 238,597 - Psyr_0223 0.84 +0.4 238,597 - Psyr_0223 0.84 -0.1 238,597 - Psyr_0223 0.84 +0.4 238,628 - Psyr_0223 0.86 -1.4 238,628 - Psyr_0223 0.86 +2.4 238,628 - Psyr_0223 0.86 +0.1 238,673 + Psyr_0223 0.89 -0.9 238,674 - Psyr_0223 0.89 +0.9 238,674 - Psyr_0223 0.89 +0.3 238,728 + +0.7 238,728 + -0.1 238,728 + -0.4 238,728 + -0.2 238,728 + +3.8 238,728 + +0.6 238,728 + +1.9 238,729 - -0.3 238,729 - -0.9 238,729 - -1.1 238,729 - +0.4 238,729 - -0.2 238,733 + -0.1 238,733 + +0.6 238,733 + +0.2 238,733 + -2.0 238,733 + -1.1 238,734 - -1.1 238,734 - +0.3 238,734 - +0.3 238,734 - -0.5 238,734 - -2.3 238,734 - +4.6 238,734 - +0.0 238,734 - -2.6 238,734 - +2.9 238,852 + +0.0 238,909 + +0.9 238,910 - -1.3 238,918 + -1.2 238,918 + +1.0 238,918 + +0.3 238,919 - -0.1 238,919 - -2.9 239,690 + -2.5 239,694 + -0.7 239,694 + -0.1 239,694 + +0.1 239,694 + -0.5 239,694 + -1.6 239,694 + +0.3 239,694 + +1.3 239,694 + -0.1 239,694 + +0.3 239,694 + -0.1 239,694 + +0.3 239,695 - -0.6 239,695 - -0.0 239,695 - -0.1 239,695 - +1.4 239,695 - -0.9 239,698 + +4.4 239,724 + +0.2 239,724 + +0.1 239,724 + -0.6 239,724 + -0.9 239,724 + +0.4 239,724 + +0.4 239,725 - -0.2 239,725 - +0.1 239,725 - +0.2 239,758 + Psyr_0226 0.18 -0.6 239,758 + Psyr_0226 0.18 -1.5 239,759 - Psyr_0226 0.18 -0.0 239,759 - Psyr_0226 0.18 +0.9
Or see this region's nucleotide sequence