Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3107 and Psyr_3108 are separated by 93 nucleotides Psyr_3108 and Psyr_3109 overlap by 25 nucleotides Psyr_3109 and Psyr_3110 are separated by 168 nucleotides Psyr_3110 and Psyr_3111 are separated by 10 nucleotides
Psyr_3107: Psyr_3107 - conserved hypothetical protein, at 3,725,187 to 3,725,471
_3107
Psyr_3108: Psyr_3108 - conserved hypothetical protein, at 3,725,565 to 3,725,867
_3108
Psyr_3109: Psyr_3109 - conserved hypothetical protein, at 3,725,843 to 3,727,546
_3109
Psyr_3110: Psyr_3110 - Nucleoside 2-deoxyribosyltransferase, at 3,727,715 to 3,728,233
_3110
Psyr_3111: Psyr_3111 - transcriptional regulator, LysR family, at 3,728,244 to 3,729,146
_3111
Position (kb)
3725
3726
3727
3728 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3724.923 kb on + strand at 3724.923 kb on + strand at 3724.924 kb on - strand at 3724.932 kb on - strand at 3724.981 kb on - strand at 3724.987 kb on - strand at 3724.987 kb on - strand at 3725.071 kb on - strand at 3725.103 kb on - strand at 3725.107 kb on - strand at 3725.132 kb on + strand at 3725.132 kb on + strand at 3725.133 kb on - strand at 3725.161 kb on + strand at 3725.162 kb on - strand at 3725.181 kb on - strand at 3725.237 kb on - strand, within Psyr_3107 at 3725.483 kb on - strand at 3725.483 kb on - strand at 3725.547 kb on - strand at 3725.547 kb on - strand at 3725.547 kb on - strand at 3725.550 kb on + strand at 3725.576 kb on + strand at 3725.576 kb on + strand at 3725.577 kb on - strand at 3725.577 kb on - strand at 3725.577 kb on - strand at 3725.590 kb on + strand at 3725.922 kb on + strand at 3725.922 kb on + strand at 3725.923 kb on - strand at 3725.923 kb on - strand at 3726.083 kb on + strand, within Psyr_3109 at 3726.083 kb on + strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.084 kb on - strand, within Psyr_3109 at 3726.139 kb on - strand, within Psyr_3109 at 3726.183 kb on - strand, within Psyr_3109 at 3726.183 kb on - strand, within Psyr_3109 at 3726.240 kb on + strand, within Psyr_3109 at 3726.240 kb on + strand, within Psyr_3109 at 3726.240 kb on + strand, within Psyr_3109 at 3726.241 kb on - strand, within Psyr_3109 at 3726.297 kb on + strand, within Psyr_3109 at 3726.312 kb on + strand, within Psyr_3109 at 3726.312 kb on + strand, within Psyr_3109 at 3726.426 kb on + strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.427 kb on - strand, within Psyr_3109 at 3726.468 kb on - strand, within Psyr_3109 at 3726.718 kb on - strand, within Psyr_3109 at 3726.718 kb on - strand, within Psyr_3109 at 3726.732 kb on + strand, within Psyr_3109 at 3727.041 kb on + strand, within Psyr_3109 at 3727.041 kb on + strand, within Psyr_3109 at 3727.042 kb on - strand, within Psyr_3109 at 3727.042 kb on - strand, within Psyr_3109 at 3727.140 kb on + strand, within Psyr_3109 at 3727.140 kb on + strand, within Psyr_3109 at 3727.513 kb on - strand at 3727.515 kb on + strand at 3727.541 kb on - strand at 3727.541 kb on - strand at 3727.562 kb on - strand at 3727.562 kb on - strand at 3727.653 kb on + strand at 3727.654 kb on - strand at 3727.677 kb on - strand at 3727.694 kb on - strand at 3727.694 kb on - strand at 3727.714 kb on + strand at 3727.733 kb on + strand at 3727.734 kb on - strand at 3727.734 kb on - strand at 3727.734 kb on - strand at 3727.784 kb on + strand, within Psyr_3110 at 3727.826 kb on + strand, within Psyr_3110 at 3728.024 kb on + strand, within Psyr_3110 at 3728.024 kb on + strand, within Psyr_3110 at 3728.025 kb on - strand, within Psyr_3110 at 3728.025 kb on - strand, within Psyr_3110 at 3728.025 kb on - strand, within Psyr_3110 at 3728.095 kb on + strand, within Psyr_3110 at 3728.096 kb on - strand, within Psyr_3110 at 3728.195 kb on + strand at 3728.195 kb on + strand at 3728.196 kb on - strand at 3728.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 3,724,923 + +1.4 3,724,923 + +0.8 3,724,924 - +0.6 3,724,932 - -0.8 3,724,981 - -0.6 3,724,987 - +0.3 3,724,987 - -0.9 3,725,071 - +0.7 3,725,103 - -0.1 3,725,107 - -1.0 3,725,132 + -1.7 3,725,132 + +0.6 3,725,133 - -0.5 3,725,161 + +0.9 3,725,162 - -0.4 3,725,181 - -0.7 3,725,237 - Psyr_3107 0.18 -1.8 3,725,483 - -0.5 3,725,483 - -0.6 3,725,547 - +0.3 3,725,547 - -0.7 3,725,547 - -0.3 3,725,550 + -0.5 3,725,576 + +1.3 3,725,576 + +0.7 3,725,577 - +0.8 3,725,577 - +1.5 3,725,577 - +0.9 3,725,590 + +0.8 3,725,922 + -0.7 3,725,922 + -0.7 3,725,923 - +0.2 3,725,923 - -1.4 3,726,083 + Psyr_3109 0.14 -0.0 3,726,083 + Psyr_3109 0.14 +2.4 3,726,084 - Psyr_3109 0.14 -1.8 3,726,084 - Psyr_3109 0.14 +0.9 3,726,084 - Psyr_3109 0.14 -1.8 3,726,084 - Psyr_3109 0.14 +0.1 3,726,084 - Psyr_3109 0.14 -1.2 3,726,084 - Psyr_3109 0.14 -0.4 3,726,084 - Psyr_3109 0.14 +2.2 3,726,139 - Psyr_3109 0.17 +1.8 3,726,183 - Psyr_3109 0.20 -0.2 3,726,183 - Psyr_3109 0.20 -1.3 3,726,240 + Psyr_3109 0.23 -0.7 3,726,240 + Psyr_3109 0.23 -0.7 3,726,240 + Psyr_3109 0.23 -0.8 3,726,241 - Psyr_3109 0.23 +1.9 3,726,297 + Psyr_3109 0.27 +4.4 3,726,312 + Psyr_3109 0.28 -0.1 3,726,312 + Psyr_3109 0.28 +0.1 3,726,426 + Psyr_3109 0.34 +0.1 3,726,427 - Psyr_3109 0.34 +0.7 3,726,427 - Psyr_3109 0.34 -0.5 3,726,427 - Psyr_3109 0.34 -0.0 3,726,468 - Psyr_3109 0.37 -0.0 3,726,718 - Psyr_3109 0.51 -0.3 3,726,718 - Psyr_3109 0.51 -0.5 3,726,732 + Psyr_3109 0.52 -0.1 3,727,041 + Psyr_3109 0.70 +0.9 3,727,041 + Psyr_3109 0.70 +0.1 3,727,042 - Psyr_3109 0.70 +0.1 3,727,042 - Psyr_3109 0.70 +0.7 3,727,140 + Psyr_3109 0.76 +1.1 3,727,140 + Psyr_3109 0.76 -0.7 3,727,513 - +1.4 3,727,515 + +1.6 3,727,541 - -0.0 3,727,541 - +0.4 3,727,562 - +1.4 3,727,562 - +0.7 3,727,653 + -1.1 3,727,654 - +0.4 3,727,677 - -0.3 3,727,694 - -0.1 3,727,694 - -0.1 3,727,714 + +0.9 3,727,733 + +2.5 3,727,734 - +0.5 3,727,734 - -0.3 3,727,734 - -0.2 3,727,784 + Psyr_3110 0.13 -0.8 3,727,826 + Psyr_3110 0.21 -1.3 3,728,024 + Psyr_3110 0.60 +0.7 3,728,024 + Psyr_3110 0.60 +0.1 3,728,025 - Psyr_3110 0.60 -1.2 3,728,025 - Psyr_3110 0.60 -1.3 3,728,025 - Psyr_3110 0.60 +1.6 3,728,095 + Psyr_3110 0.73 -1.9 3,728,096 - Psyr_3110 0.73 -0.3 3,728,195 + -0.3 3,728,195 + +0.4 3,728,196 - -1.7 3,728,318 - +0.4
Or see this region's nucleotide sequence