Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3290 and Psyr_3291 are separated by 301 nucleotides Psyr_3291 and Psyr_3292 are separated by 98 nucleotides Psyr_3292 and Psyr_3293 are separated by 130 nucleotides Psyr_3293 and Psyr_3294 are separated by 69 nucleotides
Psyr_3290: Psyr_3290 - 3-hydroxyacyl-CoA dehydrogenase / short chain enoyl-CoA hydratase, at 3,936,776 to 3,938,941
_3290
Psyr_3291: Psyr_3291 - hypothetical protein, at 3,939,243 to 3,939,536
_3291
Psyr_3292: Psyr_3292 - conserved hypothetical protein, at 3,939,635 to 3,940,072
_3292
Psyr_3293: Psyr_3293 - UspA, at 3,940,203 to 3,940,643
_3293
Psyr_3294: Psyr_3294 - ABC transporter, at 3,940,713 to 3,942,623
_3294
Position (kb)
3939
3940
3941 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3938.935 kb on - strand at 3938.959 kb on - strand at 3939.260 kb on - strand at 3939.304 kb on + strand, within Psyr_3291 at 3939.304 kb on + strand, within Psyr_3291 at 3939.304 kb on + strand, within Psyr_3291 at 3939.305 kb on - strand, within Psyr_3291 at 3939.307 kb on + strand, within Psyr_3291 at 3939.374 kb on + strand, within Psyr_3291 at 3939.374 kb on + strand, within Psyr_3291 at 3939.375 kb on - strand, within Psyr_3291 at 3939.462 kb on + strand, within Psyr_3291 at 3939.616 kb on + strand at 3939.703 kb on - strand, within Psyr_3292 at 3939.738 kb on + strand, within Psyr_3292 at 3939.879 kb on + strand, within Psyr_3292 at 3939.880 kb on - strand, within Psyr_3292 at 3940.073 kb on - strand at 3940.166 kb on + strand at 3940.209 kb on + strand at 3940.210 kb on - strand at 3940.210 kb on - strand at 3940.561 kb on + strand, within Psyr_3293 at 3940.817 kb on - strand at 3940.915 kb on + strand, within Psyr_3294 at 3940.916 kb on - strand, within Psyr_3294 at 3940.916 kb on - strand, within Psyr_3294 at 3940.916 kb on - strand, within Psyr_3294 at 3940.916 kb on - strand, within Psyr_3294
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 3,938,935 - -1.6 3,938,959 - -0.4 3,939,260 - -0.3 3,939,304 + Psyr_3291 0.21 +0.5 3,939,304 + Psyr_3291 0.21 -0.1 3,939,304 + Psyr_3291 0.21 +2.1 3,939,305 - Psyr_3291 0.21 +0.5 3,939,307 + Psyr_3291 0.22 -0.1 3,939,374 + Psyr_3291 0.45 -0.3 3,939,374 + Psyr_3291 0.45 -0.3 3,939,375 - Psyr_3291 0.45 +1.3 3,939,462 + Psyr_3291 0.74 +2.9 3,939,616 + +1.1 3,939,703 - Psyr_3292 0.16 -0.8 3,939,738 + Psyr_3292 0.24 -2.4 3,939,879 + Psyr_3292 0.56 -0.2 3,939,880 - Psyr_3292 0.56 -3.3 3,940,073 - +0.1 3,940,166 + +1.0 3,940,209 + -2.1 3,940,210 - -0.6 3,940,210 - -1.1 3,940,561 + Psyr_3293 0.81 -1.5 3,940,817 - +0.5 3,940,915 + Psyr_3294 0.11 -0.6 3,940,916 - Psyr_3294 0.11 +0.1 3,940,916 - Psyr_3294 0.11 +0.3 3,940,916 - Psyr_3294 0.11 -0.2 3,940,916 - Psyr_3294 0.11 -1.6
Or see this region's nucleotide sequence