Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2614 and Psyr_2615 overlap by 4 nucleotides Psyr_2615 and Psyr_2616 are separated by 425 nucleotides
Psyr_2614: Psyr_2614 - Amino acid adenylation, at 3,098,777 to 3,114,895
_2614
Psyr_2615: Psyr_2615 - Amino acid adenylation, at 3,114,892 to 3,131,301
_2615
Psyr_2616: Psyr_2616 - Amino acid adenylation, at 3,131,727 to 3,172,340
_2616
Position (kb) 3114
3115
3116
3117
3118
3119
3120
3121
3122
3123
3124
3125
3126
3127
3128
3129
3130
3131
3132 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 3114.026 kb on + strand at 3114.105 kb on - strand at 3114.232 kb on + strand at 3114.461 kb on + strand at 3114.462 kb on - strand at 3114.462 kb on - strand at 3114.464 kb on + strand at 3114.465 kb on - strand at 3114.465 kb on - strand at 3114.539 kb on + strand at 3114.539 kb on + strand at 3114.539 kb on + strand at 3114.539 kb on + strand at 3114.540 kb on - strand at 3114.540 kb on - strand at 3114.540 kb on - strand at 3114.541 kb on + strand at 3114.542 kb on - strand at 3114.557 kb on + strand at 3114.682 kb on - strand at 3114.858 kb on - strand at 3115.063 kb on + strand at 3115.066 kb on + strand at 3115.067 kb on - strand at 3115.228 kb on + strand at 3115.229 kb on - strand at 3115.229 kb on - strand at 3115.294 kb on + strand at 3115.298 kb on + strand at 3115.298 kb on + strand at 3115.732 kb on + strand at 3115.732 kb on + strand at 3115.732 kb on + strand at 3115.732 kb on + strand at 3116.031 kb on + strand at 3116.032 kb on - strand at 3116.074 kb on - strand at 3116.313 kb on - strand at 3116.663 kb on - strand, within Psyr_2615 at 3116.809 kb on + strand, within Psyr_2615 at 3116.810 kb on - strand, within Psyr_2615 at 3117.016 kb on + strand, within Psyr_2615 at 3117.025 kb on + strand, within Psyr_2615 at 3117.026 kb on - strand, within Psyr_2615 at 3117.422 kb on - strand, within Psyr_2615 at 3117.445 kb on + strand, within Psyr_2615 at 3117.524 kb on - strand, within Psyr_2615 at 3117.706 kb on + strand, within Psyr_2615 at 3117.880 kb on + strand, within Psyr_2615 at 3117.883 kb on + strand, within Psyr_2615 at 3117.958 kb on + strand, within Psyr_2615 at 3117.976 kb on + strand, within Psyr_2615 at 3118.241 kb on + strand, within Psyr_2615 at 3118.242 kb on - strand, within Psyr_2615 at 3118.462 kb on + strand, within Psyr_2615 at 3118.495 kb on + strand, within Psyr_2615 at 3118.529 kb on - strand, within Psyr_2615 at 3118.532 kb on + strand, within Psyr_2615 at 3118.728 kb on + strand, within Psyr_2615 at 3118.729 kb on - strand, within Psyr_2615 at 3118.729 kb on - strand, within Psyr_2615 at 3118.730 kb on + strand, within Psyr_2615 at 3118.731 kb on - strand, within Psyr_2615 at 3118.808 kb on + strand, within Psyr_2615 at 3118.808 kb on + strand, within Psyr_2615 at 3118.809 kb on - strand, within Psyr_2615 at 3118.809 kb on - strand, within Psyr_2615 at 3118.812 kb on + strand, within Psyr_2615 at 3118.813 kb on - strand, within Psyr_2615 at 3118.966 kb on + strand, within Psyr_2615 at 3118.967 kb on - strand, within Psyr_2615 at 3119.075 kb on + strand, within Psyr_2615 at 3119.076 kb on - strand, within Psyr_2615 at 3119.076 kb on - strand, within Psyr_2615 at 3119.314 kb on - strand, within Psyr_2615 at 3120.075 kb on - strand, within Psyr_2615 at 3120.088 kb on + strand, within Psyr_2615 at 3120.088 kb on + strand, within Psyr_2615 at 3120.089 kb on - strand, within Psyr_2615 at 3120.089 kb on - strand, within Psyr_2615 at 3120.090 kb on + strand, within Psyr_2615 at 3120.090 kb on + strand, within Psyr_2615 at 3120.090 kb on + strand, within Psyr_2615 at 3120.090 kb on + strand, within Psyr_2615 at 3120.091 kb on - strand, within Psyr_2615 at 3120.091 kb on - strand, within Psyr_2615 at 3120.091 kb on - strand, within Psyr_2615 at 3120.263 kb on - strand, within Psyr_2615 at 3120.682 kb on + strand, within Psyr_2615 at 3120.761 kb on - strand, within Psyr_2615 at 3121.195 kb on + strand, within Psyr_2615 at 3121.196 kb on - strand, within Psyr_2615 at 3121.196 kb on - strand, within Psyr_2615 at 3121.196 kb on - strand, within Psyr_2615 at 3121.582 kb on + strand, within Psyr_2615 at 3121.582 kb on + strand, within Psyr_2615 at 3121.583 kb on - strand, within Psyr_2615 at 3121.717 kb on + strand, within Psyr_2615 at 3122.188 kb on + strand, within Psyr_2615 at 3122.188 kb on + strand, within Psyr_2615 at 3122.188 kb on + strand, within Psyr_2615 at 3122.189 kb on - strand, within Psyr_2615 at 3122.189 kb on - strand, within Psyr_2615 at 3122.189 kb on - strand, within Psyr_2615 at 3122.841 kb on + strand, within Psyr_2615 at 3122.842 kb on - strand, within Psyr_2615 at 3122.865 kb on - strand, within Psyr_2615 at 3122.865 kb on - strand, within Psyr_2615 at 3122.865 kb on - strand, within Psyr_2615 at 3122.887 kb on + strand, within Psyr_2615 at 3122.887 kb on + strand, within Psyr_2615 at 3122.888 kb on - strand, within Psyr_2615 at 3122.888 kb on - strand, within Psyr_2615 at 3122.991 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.266 kb on - strand, within Psyr_2615 at 3123.269 kb on + strand, within Psyr_2615 at 3123.269 kb on + strand, within Psyr_2615 at 3123.270 kb on - strand, within Psyr_2615 at 3123.460 kb on + strand, within Psyr_2615 at 3123.461 kb on - strand, within Psyr_2615 at 3123.461 kb on - strand, within Psyr_2615 at 3123.469 kb on + strand, within Psyr_2615 at 3123.469 kb on + strand, within Psyr_2615 at 3123.469 kb on + strand, within Psyr_2615 at 3123.469 kb on + strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.470 kb on - strand, within Psyr_2615 at 3123.988 kb on + strand, within Psyr_2615 at 3123.988 kb on + strand, within Psyr_2615 at 3123.989 kb on - strand, within Psyr_2615 at 3123.989 kb on - strand, within Psyr_2615 at 3124.042 kb on + strand, within Psyr_2615 at 3124.042 kb on + strand, within Psyr_2615 at 3124.042 kb on + strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.043 kb on - strand, within Psyr_2615 at 3124.073 kb on - strand, within Psyr_2615 at 3124.148 kb on - strand, within Psyr_2615 at 3124.345 kb on + strand, within Psyr_2615 at 3124.345 kb on + strand, within Psyr_2615 at 3124.345 kb on + strand, within Psyr_2615 at 3124.345 kb on + strand, within Psyr_2615 at 3124.346 kb on - strand, within Psyr_2615 at 3124.424 kb on - strand, within Psyr_2615 at 3124.537 kb on + strand, within Psyr_2615 at 3124.538 kb on - strand, within Psyr_2615 at 3124.677 kb on + strand, within Psyr_2615 at 3124.678 kb on - strand, within Psyr_2615 at 3125.416 kb on + strand, within Psyr_2615 at 3125.416 kb on + strand, within Psyr_2615 at 3125.416 kb on + strand, within Psyr_2615 at 3125.462 kb on + strand, within Psyr_2615 at 3125.687 kb on + strand, within Psyr_2615 at 3125.758 kb on - strand, within Psyr_2615 at 3126.112 kb on + strand, within Psyr_2615 at 3126.113 kb on - strand, within Psyr_2615 at 3126.113 kb on - strand, within Psyr_2615 at 3126.115 kb on - strand, within Psyr_2615 at 3126.533 kb on - strand, within Psyr_2615 at 3126.619 kb on + strand, within Psyr_2615 at 3126.620 kb on - strand, within Psyr_2615 at 3126.694 kb on + strand, within Psyr_2615 at 3126.703 kb on + strand, within Psyr_2615 at 3127.117 kb on - strand, within Psyr_2615 at 3127.127 kb on + strand, within Psyr_2615 at 3127.136 kb on - strand, within Psyr_2615 at 3127.136 kb on - strand, within Psyr_2615 at 3127.136 kb on - strand, within Psyr_2615 at 3127.169 kb on - strand, within Psyr_2615 at 3127.169 kb on - strand, within Psyr_2615 at 3127.300 kb on + strand, within Psyr_2615 at 3127.384 kb on - strand, within Psyr_2615 at 3127.453 kb on + strand, within Psyr_2615 at 3127.603 kb on + strand, within Psyr_2615 at 3127.603 kb on + strand, within Psyr_2615 at 3127.604 kb on - strand, within Psyr_2615 at 3127.604 kb on - strand, within Psyr_2615 at 3127.604 kb on - strand, within Psyr_2615 at 3127.604 kb on - strand, within Psyr_2615 at 3127.682 kb on - strand, within Psyr_2615 at 3127.700 kb on - strand, within Psyr_2615 at 3128.118 kb on - strand, within Psyr_2615 at 3129.187 kb on - strand, within Psyr_2615 at 3129.371 kb on - strand, within Psyr_2615 at 3129.371 kb on - strand, within Psyr_2615 at 3129.753 kb on - strand at 3129.967 kb on + strand at 3129.968 kb on - strand at 3130.388 kb on - strand at 3130.388 kb on - strand at 3130.388 kb on - strand at 3130.519 kb on + strand at 3130.519 kb on + strand at 3130.520 kb on - strand at 3130.538 kb on + strand at 3130.625 kb on - strand at 3130.900 kb on + strand at 3130.901 kb on - strand at 3130.901 kb on - strand at 3130.918 kb on + strand at 3130.919 kb on - strand at 3130.919 kb on - strand at 3130.919 kb on - strand at 3130.921 kb on - strand at 3132.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 3,114,026 + +0.9 3,114,105 - -0.2 3,114,232 + +0.3 3,114,461 + -0.4 3,114,462 - +0.1 3,114,462 - +0.7 3,114,464 + -0.6 3,114,465 - -1.1 3,114,465 - -0.0 3,114,539 + +0.4 3,114,539 + -1.1 3,114,539 + +1.6 3,114,539 + +0.4 3,114,540 - -0.4 3,114,540 - -1.2 3,114,540 - -1.0 3,114,541 + +0.7 3,114,542 - -0.4 3,114,557 + -0.1 3,114,682 - +1.3 3,114,858 - -0.5 3,115,063 + -0.2 3,115,066 + -1.2 3,115,067 - +1.3 3,115,228 + +0.3 3,115,229 - -1.3 3,115,229 - +0.4 3,115,294 + -1.2 3,115,298 + -0.8 3,115,298 + -0.7 3,115,732 + -0.6 3,115,732 + +0.7 3,115,732 + +0.4 3,115,732 + +1.0 3,116,031 + +0.6 3,116,032 - +1.3 3,116,074 - -0.6 3,116,313 - -0.1 3,116,663 - Psyr_2615 0.11 -0.9 3,116,809 + Psyr_2615 0.12 -1.3 3,116,810 - Psyr_2615 0.12 -0.5 3,117,016 + Psyr_2615 0.13 -2.4 3,117,025 + Psyr_2615 0.13 -0.9 3,117,026 - Psyr_2615 0.13 +1.0 3,117,422 - Psyr_2615 0.15 +1.3 3,117,445 + Psyr_2615 0.16 -1.4 3,117,524 - Psyr_2615 0.16 -0.5 3,117,706 + Psyr_2615 0.17 +1.1 3,117,880 + Psyr_2615 0.18 +0.3 3,117,883 + Psyr_2615 0.18 +0.0 3,117,958 + Psyr_2615 0.19 -0.6 3,117,976 + Psyr_2615 0.19 -0.5 3,118,241 + Psyr_2615 0.20 -0.2 3,118,242 - Psyr_2615 0.20 -0.6 3,118,462 + Psyr_2615 0.22 -1.1 3,118,495 + Psyr_2615 0.22 -0.3 3,118,529 - Psyr_2615 0.22 -0.9 3,118,532 + Psyr_2615 0.22 -1.4 3,118,728 + Psyr_2615 0.23 +0.0 3,118,729 - Psyr_2615 0.23 -0.6 3,118,729 - Psyr_2615 0.23 -0.1 3,118,730 + Psyr_2615 0.23 -1.7 3,118,731 - Psyr_2615 0.23 +1.8 3,118,808 + Psyr_2615 0.24 +0.5 3,118,808 + Psyr_2615 0.24 +0.3 3,118,809 - Psyr_2615 0.24 +0.9 3,118,809 - Psyr_2615 0.24 +0.6 3,118,812 + Psyr_2615 0.24 +3.3 3,118,813 - Psyr_2615 0.24 +0.4 3,118,966 + Psyr_2615 0.25 -1.1 3,118,967 - Psyr_2615 0.25 -0.9 3,119,075 + Psyr_2615 0.25 +3.6 3,119,076 - Psyr_2615 0.25 +0.8 3,119,076 - Psyr_2615 0.25 +0.1 3,119,314 - Psyr_2615 0.27 +2.3 3,120,075 - Psyr_2615 0.32 -0.0 3,120,088 + Psyr_2615 0.32 -2.1 3,120,088 + Psyr_2615 0.32 -1.8 3,120,089 - Psyr_2615 0.32 -2.4 3,120,089 - Psyr_2615 0.32 +0.1 3,120,090 + Psyr_2615 0.32 -2.4 3,120,090 + Psyr_2615 0.32 +0.9 3,120,090 + Psyr_2615 0.32 -1.9 3,120,090 + Psyr_2615 0.32 +0.3 3,120,091 - Psyr_2615 0.32 +0.9 3,120,091 - Psyr_2615 0.32 +0.5 3,120,091 - Psyr_2615 0.32 +1.4 3,120,263 - Psyr_2615 0.33 +1.0 3,120,682 + Psyr_2615 0.35 -0.7 3,120,761 - Psyr_2615 0.36 +0.4 3,121,195 + Psyr_2615 0.38 +1.3 3,121,196 - Psyr_2615 0.38 +2.0 3,121,196 - Psyr_2615 0.38 +0.2 3,121,196 - Psyr_2615 0.38 +2.3 3,121,582 + Psyr_2615 0.41 -0.1 3,121,582 + Psyr_2615 0.41 +1.6 3,121,583 - Psyr_2615 0.41 +1.5 3,121,717 + Psyr_2615 0.42 +0.6 3,122,188 + Psyr_2615 0.44 +0.1 3,122,188 + Psyr_2615 0.44 -0.9 3,122,188 + Psyr_2615 0.44 -0.5 3,122,189 - Psyr_2615 0.44 -0.7 3,122,189 - Psyr_2615 0.44 +0.8 3,122,189 - Psyr_2615 0.44 +2.3 3,122,841 + Psyr_2615 0.48 -0.6 3,122,842 - Psyr_2615 0.48 +1.8 3,122,865 - Psyr_2615 0.49 -0.9 3,122,865 - Psyr_2615 0.49 -1.3 3,122,865 - Psyr_2615 0.49 +0.1 3,122,887 + Psyr_2615 0.49 +1.6 3,122,887 + Psyr_2615 0.49 -1.0 3,122,888 - Psyr_2615 0.49 -0.5 3,122,888 - Psyr_2615 0.49 -0.1 3,122,991 - Psyr_2615 0.49 +1.4 3,123,266 - Psyr_2615 0.51 +2.3 3,123,266 - Psyr_2615 0.51 -0.6 3,123,266 - Psyr_2615 0.51 +2.3 3,123,266 - Psyr_2615 0.51 +0.9 3,123,266 - Psyr_2615 0.51 +0.5 3,123,266 - Psyr_2615 0.51 +0.1 3,123,266 - Psyr_2615 0.51 -1.9 3,123,269 + Psyr_2615 0.51 -0.4 3,123,269 + Psyr_2615 0.51 -0.3 3,123,270 - Psyr_2615 0.51 -0.6 3,123,460 + Psyr_2615 0.52 -0.6 3,123,461 - Psyr_2615 0.52 -0.7 3,123,461 - Psyr_2615 0.52 +0.1 3,123,469 + Psyr_2615 0.52 +0.4 3,123,469 + Psyr_2615 0.52 -0.2 3,123,469 + Psyr_2615 0.52 +1.3 3,123,469 + Psyr_2615 0.52 -0.2 3,123,470 - Psyr_2615 0.52 -1.1 3,123,470 - Psyr_2615 0.52 -2.6 3,123,470 - Psyr_2615 0.52 +0.4 3,123,470 - Psyr_2615 0.52 -2.6 3,123,470 - Psyr_2615 0.52 -0.7 3,123,470 - Psyr_2615 0.52 -1.6 3,123,988 + Psyr_2615 0.55 +0.2 3,123,988 + Psyr_2615 0.55 +0.2 3,123,989 - Psyr_2615 0.55 +0.9 3,123,989 - Psyr_2615 0.55 +0.9 3,124,042 + Psyr_2615 0.56 -1.8 3,124,042 + Psyr_2615 0.56 -0.3 3,124,042 + Psyr_2615 0.56 -1.0 3,124,043 - Psyr_2615 0.56 -0.9 3,124,043 - Psyr_2615 0.56 +1.6 3,124,043 - Psyr_2615 0.56 -0.2 3,124,043 - Psyr_2615 0.56 +4.1 3,124,043 - Psyr_2615 0.56 +0.4 3,124,043 - Psyr_2615 0.56 +0.6 3,124,073 - Psyr_2615 0.56 -0.6 3,124,148 - Psyr_2615 0.56 +0.2 3,124,345 + Psyr_2615 0.58 +0.5 3,124,345 + Psyr_2615 0.58 +0.3 3,124,345 + Psyr_2615 0.58 +1.3 3,124,345 + Psyr_2615 0.58 +0.7 3,124,346 - Psyr_2615 0.58 -0.7 3,124,424 - Psyr_2615 0.58 +1.3 3,124,537 + Psyr_2615 0.59 -0.4 3,124,538 - Psyr_2615 0.59 +0.6 3,124,677 + Psyr_2615 0.60 -1.0 3,124,678 - Psyr_2615 0.60 +1.1 3,125,416 + Psyr_2615 0.64 -0.5 3,125,416 + Psyr_2615 0.64 +0.2 3,125,416 + Psyr_2615 0.64 -0.3 3,125,462 + Psyr_2615 0.64 -0.3 3,125,687 + Psyr_2615 0.66 -2.6 3,125,758 - Psyr_2615 0.66 +0.0 3,126,112 + Psyr_2615 0.68 -1.0 3,126,113 - Psyr_2615 0.68 +1.1 3,126,113 - Psyr_2615 0.68 -0.7 3,126,115 - Psyr_2615 0.68 -0.2 3,126,533 - Psyr_2615 0.71 +0.3 3,126,619 + Psyr_2615 0.71 -0.2 3,126,620 - Psyr_2615 0.71 +0.3 3,126,694 + Psyr_2615 0.72 +0.0 3,126,703 + Psyr_2615 0.72 +1.1 3,127,117 - Psyr_2615 0.74 -0.5 3,127,127 + Psyr_2615 0.75 -0.6 3,127,136 - Psyr_2615 0.75 -0.5 3,127,136 - Psyr_2615 0.75 +0.3 3,127,136 - Psyr_2615 0.75 +0.8 3,127,169 - Psyr_2615 0.75 +1.2 3,127,169 - Psyr_2615 0.75 +3.3 3,127,300 + Psyr_2615 0.76 -2.7 3,127,384 - Psyr_2615 0.76 -1.3 3,127,453 + Psyr_2615 0.77 +0.4 3,127,603 + Psyr_2615 0.77 -1.4 3,127,603 + Psyr_2615 0.77 +0.0 3,127,604 - Psyr_2615 0.77 -0.5 3,127,604 - Psyr_2615 0.77 -0.5 3,127,604 - Psyr_2615 0.77 +1.3 3,127,604 - Psyr_2615 0.77 -1.2 3,127,682 - Psyr_2615 0.78 -1.7 3,127,700 - Psyr_2615 0.78 +0.3 3,128,118 - Psyr_2615 0.81 -0.3 3,129,187 - Psyr_2615 0.87 -0.5 3,129,371 - Psyr_2615 0.88 -0.1 3,129,371 - Psyr_2615 0.88 +2.1 3,129,753 - +0.1 3,129,967 + -0.4 3,129,968 - -0.5 3,130,388 - -0.3 3,130,388 - +1.8 3,130,388 - -0.3 3,130,519 + +0.2 3,130,519 + -0.8 3,130,520 - -0.1 3,130,538 + -1.1 3,130,625 - +0.4 3,130,900 + -0.6 3,130,901 - +3.8 3,130,901 - -0.0 3,130,918 + +0.5 3,130,919 - +0.4 3,130,919 - -0.3 3,130,919 - -1.3 3,130,921 - +0.4 3,132,022 - -0.4
Or see this region's nucleotide sequence