Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2119 and Psyr_2120 are separated by 57 nucleotides Psyr_2120 and Psyr_2121 are separated by 52 nucleotides Psyr_2121 and Psyr_2122 are separated by 141 nucleotides Psyr_2122 and Psyr_2123 are separated by 11 nucleotides
Psyr_2119: Psyr_2119 - Acyltransferase 3, at 2,477,478 to 2,478,548
_2119
Psyr_2120: Psyr_2120 - conserved hypothetical protein, at 2,478,606 to 2,478,848
_2120
Psyr_2121: Psyr_2121 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,478,901 to 2,479,821
_2121
Psyr_2122: Psyr_2122 - protocatechuate 3,4-dioxygenase, beta subunit, at 2,479,963 to 2,480,682
_2122
Psyr_2123: Psyr_2123 - protocatechuate 3,4-dioxygenase, alpha subunit, at 2,480,694 to 2,481,296
_2123
Position (kb)
2478
2479
2480 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2477.904 kb on + strand, within Psyr_2119 at 2477.904 kb on + strand, within Psyr_2119 at 2477.904 kb on + strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2478.015 kb on + strand, within Psyr_2119 at 2478.015 kb on + strand, within Psyr_2119 at 2478.049 kb on - strand, within Psyr_2119 at 2478.094 kb on - strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.327 kb on + strand, within Psyr_2119 at 2478.336 kb on + strand, within Psyr_2119 at 2478.336 kb on + strand, within Psyr_2119 at 2478.336 kb on + strand, within Psyr_2119 at 2478.336 kb on + strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.337 kb on - strand, within Psyr_2119 at 2478.408 kb on + strand, within Psyr_2119 at 2478.480 kb on + strand at 2478.480 kb on + strand at 2478.481 kb on - strand at 2478.563 kb on + strand at 2478.568 kb on + strand at 2478.751 kb on - strand, within Psyr_2120 at 2478.906 kb on + strand at 2479.189 kb on - strand, within Psyr_2121 at 2479.206 kb on - strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.403 kb on - strand, within Psyr_2121 at 2479.609 kb on + strand, within Psyr_2121 at 2479.610 kb on - strand, within Psyr_2121 at 2479.702 kb on - strand, within Psyr_2121 at 2479.872 kb on - strand at 2480.035 kb on + strand, within Psyr_2122 at 2480.036 kb on - strand, within Psyr_2122 at 2480.036 kb on - strand, within Psyr_2122 at 2480.036 kb on - strand, within Psyr_2122 at 2480.196 kb on + strand, within Psyr_2122 at 2480.197 kb on - strand, within Psyr_2122 at 2480.308 kb on + strand, within Psyr_2122 at 2480.309 kb on - strand, within Psyr_2122 at 2480.374 kb on + strand, within Psyr_2122 at 2480.374 kb on + strand, within Psyr_2122 at 2480.374 kb on + strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.500 kb on + strand, within Psyr_2122 at 2480.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 2,477,904 + Psyr_2119 0.40 +0.6 2,477,904 + Psyr_2119 0.40 -0.2 2,477,904 + Psyr_2119 0.40 +1.7 2,477,905 - Psyr_2119 0.40 -0.9 2,477,905 - Psyr_2119 0.40 -1.1 2,478,015 + Psyr_2119 0.50 -0.4 2,478,015 + Psyr_2119 0.50 +1.2 2,478,049 - Psyr_2119 0.53 -0.5 2,478,094 - Psyr_2119 0.58 +0.4 2,478,192 + Psyr_2119 0.67 -0.5 2,478,192 + Psyr_2119 0.67 +1.2 2,478,192 + Psyr_2119 0.67 -3.0 2,478,193 - Psyr_2119 0.67 +0.2 2,478,193 - Psyr_2119 0.67 +0.1 2,478,193 - Psyr_2119 0.67 +0.7 2,478,193 - Psyr_2119 0.67 +1.5 2,478,327 + Psyr_2119 0.79 -0.3 2,478,336 + Psyr_2119 0.80 -0.2 2,478,336 + Psyr_2119 0.80 -0.7 2,478,336 + Psyr_2119 0.80 +1.8 2,478,336 + Psyr_2119 0.80 +0.4 2,478,337 - Psyr_2119 0.80 -1.6 2,478,337 - Psyr_2119 0.80 -2.5 2,478,337 - Psyr_2119 0.80 +0.5 2,478,337 - Psyr_2119 0.80 +0.4 2,478,337 - Psyr_2119 0.80 +0.5 2,478,337 - Psyr_2119 0.80 +1.9 2,478,337 - Psyr_2119 0.80 -1.9 2,478,337 - Psyr_2119 0.80 -0.4 2,478,408 + Psyr_2119 0.87 -0.1 2,478,480 + +0.1 2,478,480 + +1.0 2,478,481 - -1.0 2,478,563 + -0.5 2,478,568 + +1.2 2,478,751 - Psyr_2120 0.60 +0.4 2,478,906 + -0.9 2,479,189 - Psyr_2121 0.31 -0.6 2,479,206 - Psyr_2121 0.33 -1.9 2,479,402 + Psyr_2121 0.54 -1.0 2,479,402 + Psyr_2121 0.54 -0.8 2,479,403 - Psyr_2121 0.55 -0.0 2,479,609 + Psyr_2121 0.77 +1.3 2,479,610 - Psyr_2121 0.77 -0.8 2,479,702 - Psyr_2121 0.87 -0.3 2,479,872 - -1.3 2,480,035 + Psyr_2122 0.10 -0.8 2,480,036 - Psyr_2122 0.10 -1.2 2,480,036 - Psyr_2122 0.10 -0.5 2,480,036 - Psyr_2122 0.10 +0.5 2,480,196 + Psyr_2122 0.32 -1.1 2,480,197 - Psyr_2122 0.33 +0.3 2,480,308 + Psyr_2122 0.48 +2.2 2,480,309 - Psyr_2122 0.48 -0.1 2,480,374 + Psyr_2122 0.57 -0.0 2,480,374 + Psyr_2122 0.57 -0.2 2,480,374 + Psyr_2122 0.57 -2.1 2,480,375 - Psyr_2122 0.57 +2.2 2,480,375 - Psyr_2122 0.57 -2.5 2,480,500 + Psyr_2122 0.75 -0.2 2,480,637 + -0.8
Or see this region's nucleotide sequence