Experiment: KB with Physcion 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1320 and Psyr_1321 are separated by 242 nucleotides Psyr_1321 and Psyr_1322 are separated by 2 nucleotides Psyr_1322 and Psyr_1323 are separated by 133 nucleotides
Psyr_1320: Psyr_1320 - Peptidase M22, glycoprotease, at 1,498,812 to 1,499,486
_1320
Psyr_1321: Psyr_1321 - conserved hypothetical protein, at 1,499,729 to 1,500,040
_1321
Psyr_1322: Psyr_1322 - Protein of unknown function DUF72, at 1,500,043 to 1,500,906
_1322
Psyr_1323: Psyr_1323 - Extensin-like, C-terminal, at 1,501,040 to 1,501,729
_1323
Position (kb)
1499
1500
1501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1498.738 kb on + strand at 1498.738 kb on + strand at 1498.738 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.769 kb on + strand at 1498.770 kb on - strand at 1498.770 kb on - strand at 1498.770 kb on - strand at 1498.770 kb on - strand at 1498.770 kb on - strand at 1498.771 kb on + strand at 1498.771 kb on + strand at 1499.474 kb on + strand at 1499.474 kb on + strand at 1499.475 kb on - strand at 1499.475 kb on - strand at 1499.475 kb on - strand at 1499.589 kb on - strand at 1499.644 kb on + strand at 1499.644 kb on + strand at 1499.644 kb on + strand at 1499.644 kb on + strand at 1499.653 kb on + strand at 1499.654 kb on - strand at 1499.679 kb on + strand at 1499.680 kb on - strand at 1499.680 kb on - strand at 1499.680 kb on - strand at 1499.680 kb on - strand at 1499.680 kb on - strand at 1499.695 kb on + strand at 1499.719 kb on + strand at 1499.723 kb on + strand at 1499.723 kb on + strand at 1499.736 kb on + strand at 1499.737 kb on - strand at 1499.904 kb on + strand, within Psyr_1321 at 1499.904 kb on + strand, within Psyr_1321 at 1499.904 kb on + strand, within Psyr_1321 at 1499.905 kb on - strand, within Psyr_1321 at 1499.975 kb on + strand, within Psyr_1321 at 1499.975 kb on + strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.976 kb on - strand, within Psyr_1321 at 1499.978 kb on + strand, within Psyr_1321 at 1499.978 kb on - strand, within Psyr_1321 at 1500.059 kb on - strand at 1500.059 kb on - strand at 1500.059 kb on - strand at 1500.061 kb on + strand at 1500.061 kb on + strand at 1500.061 kb on + strand at 1500.062 kb on - strand at 1500.062 kb on - strand at 1500.110 kb on - strand at 1500.148 kb on + strand, within Psyr_1322 at 1500.190 kb on + strand, within Psyr_1322 at 1500.190 kb on + strand, within Psyr_1322 at 1500.191 kb on - strand, within Psyr_1322 at 1500.211 kb on - strand, within Psyr_1322 at 1500.233 kb on + strand, within Psyr_1322 at 1500.233 kb on + strand, within Psyr_1322 at 1500.233 kb on + strand, within Psyr_1322 at 1500.234 kb on - strand, within Psyr_1322 at 1500.234 kb on - strand, within Psyr_1322 at 1500.234 kb on - strand, within Psyr_1322 at 1500.760 kb on + strand, within Psyr_1322 at 1500.760 kb on + strand, within Psyr_1322 at 1500.761 kb on - strand, within Psyr_1322 at 1500.761 kb on - strand, within Psyr_1322 at 1500.761 kb on - strand, within Psyr_1322 at 1500.956 kb on + strand at 1500.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Physcion 10 mM remove 1,498,738 + -1.5 1,498,738 + +0.2 1,498,738 + -2.1 1,498,769 + -1.0 1,498,769 + +0.6 1,498,769 + +0.6 1,498,769 + -0.3 1,498,769 + +0.1 1,498,769 + +0.2 1,498,769 + -0.8 1,498,769 + -0.8 1,498,770 - -0.9 1,498,770 - -1.6 1,498,770 - -0.3 1,498,770 - -3.0 1,498,770 - -1.9 1,498,771 + -0.8 1,498,771 + +0.7 1,499,474 + -0.5 1,499,474 + +0.1 1,499,475 - +0.5 1,499,475 - +0.5 1,499,475 - -0.5 1,499,589 - -0.4 1,499,644 + -1.5 1,499,644 + +1.2 1,499,644 + -0.7 1,499,644 + -1.2 1,499,653 + -1.3 1,499,654 - +0.9 1,499,679 + +1.1 1,499,680 - +0.1 1,499,680 - -1.6 1,499,680 - +4.6 1,499,680 - -0.1 1,499,680 - +1.2 1,499,695 + -0.7 1,499,719 + +2.8 1,499,723 + +1.2 1,499,723 + +0.6 1,499,736 + +0.5 1,499,737 - -0.3 1,499,904 + Psyr_1321 0.56 +1.2 1,499,904 + Psyr_1321 0.56 +0.2 1,499,904 + Psyr_1321 0.56 -0.9 1,499,905 - Psyr_1321 0.56 -0.0 1,499,975 + Psyr_1321 0.79 +0.1 1,499,975 + Psyr_1321 0.79 -0.6 1,499,976 - Psyr_1321 0.79 -0.2 1,499,976 - Psyr_1321 0.79 -0.6 1,499,976 - Psyr_1321 0.79 +0.8 1,499,976 - Psyr_1321 0.79 -0.0 1,499,976 - Psyr_1321 0.79 -0.5 1,499,976 - Psyr_1321 0.79 +0.8 1,499,976 - Psyr_1321 0.79 +1.2 1,499,976 - Psyr_1321 0.79 -1.6 1,499,976 - Psyr_1321 0.79 +0.3 1,499,978 + Psyr_1321 0.80 +0.7 1,499,978 - Psyr_1321 0.80 +0.2 1,500,059 - +0.8 1,500,059 - -0.3 1,500,059 - +2.0 1,500,061 + +2.2 1,500,061 + -0.4 1,500,061 + +0.0 1,500,062 - +1.2 1,500,062 - -1.9 1,500,110 - -0.6 1,500,148 + Psyr_1322 0.12 -1.0 1,500,190 + Psyr_1322 0.17 +0.3 1,500,190 + Psyr_1322 0.17 +1.1 1,500,191 - Psyr_1322 0.17 +0.2 1,500,211 - Psyr_1322 0.19 +0.7 1,500,233 + Psyr_1322 0.22 +0.1 1,500,233 + Psyr_1322 0.22 +0.7 1,500,233 + Psyr_1322 0.22 +0.9 1,500,234 - Psyr_1322 0.22 -0.3 1,500,234 - Psyr_1322 0.22 +1.5 1,500,234 - Psyr_1322 0.22 -0.1 1,500,760 + Psyr_1322 0.83 +0.2 1,500,760 + Psyr_1322 0.83 +0.4 1,500,761 - Psyr_1322 0.83 -0.8 1,500,761 - Psyr_1322 0.83 +1.1 1,500,761 - Psyr_1322 0.83 -0.8 1,500,956 + +0.4 1,500,990 + -1.6
Or see this region's nucleotide sequence