Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00395 and PS417_00400 are separated by 56 nucleotides PS417_00400 and PS417_00405 are separated by 74 nucleotides PS417_00405 and PS417_00410 are separated by 269 nucleotides
PS417_00395: PS417_00395 - serine kinase, at 76,603 to 77,556
_00395
PS417_00400: PS417_00400 - hypothetical protein, at 77,613 to 77,903
_00400
PS417_00405: PS417_00405 - DNA polymerase I, at 77,978 to 80,755
_00405
PS417_00410: PS417_00410 - GTP-binding protein, at 81,025 to 81,666
_00410
Position (kb) 77
78
79
80
81 Strain fitness (log2 ratio) -2
-1
0
1
2 at 77.186 kb on - strand, within PS417_00395 at 77.607 kb on - strand at 77.652 kb on - strand, within PS417_00400 at 77.897 kb on + strand at 77.897 kb on + strand at 77.897 kb on + strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.906 kb on + strand at 78.806 kb on + strand, within PS417_00405 at 78.865 kb on - strand, within PS417_00405 at 78.929 kb on + strand, within PS417_00405 at 78.929 kb on + strand, within PS417_00405 at 78.930 kb on - strand, within PS417_00405 at 78.930 kb on - strand at 79.106 kb on + strand, within PS417_00405 at 79.106 kb on + strand, within PS417_00405 at 79.106 kb on + strand, within PS417_00405 at 79.110 kb on + strand, within PS417_00405 at 79.304 kb on + strand, within PS417_00405 at 79.305 kb on - strand, within PS417_00405 at 79.305 kb on - strand, within PS417_00405 at 79.305 kb on - strand, within PS417_00405 at 79.308 kb on + strand, within PS417_00405 at 79.308 kb on + strand, within PS417_00405 at 79.309 kb on - strand, within PS417_00405 at 79.309 kb on - strand, within PS417_00405 at 79.358 kb on + strand, within PS417_00405 at 79.368 kb on - strand, within PS417_00405 at 79.514 kb on - strand, within PS417_00405 at 79.886 kb on + strand, within PS417_00405 at 80.070 kb on + strand, within PS417_00405 at 80.330 kb on - strand, within PS417_00405 at 80.339 kb on + strand, within PS417_00405 at 80.339 kb on + strand, within PS417_00405 at 80.369 kb on + strand, within PS417_00405 at 80.370 kb on - strand, within PS417_00405 at 80.370 kb on - strand, within PS417_00405 at 80.408 kb on + strand, within PS417_00405 at 80.799 kb on - strand at 80.829 kb on + strand at 80.853 kb on + strand at 80.854 kb on - strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.936 kb on - strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 77,186 - PS417_00395 0.61 -1.0 77,607 - -0.8 77,652 - PS417_00400 0.13 +0.4 77,897 + +1.1 77,897 + -0.3 77,897 + -0.8 77,898 - -0.1 77,898 - -0.1 77,898 - -0.2 77,906 + +0.5 78,806 + PS417_00405 0.30 +2.3 78,865 - PS417_00405 0.32 +0.4 78,929 + PS417_00405 0.34 -0.3 78,929 + PS417_00405 0.34 +0.3 78,930 - PS417_00405 0.34 +1.3 78,930 - -0.9 79,106 + PS417_00405 0.41 -1.1 79,106 + PS417_00405 0.41 -0.7 79,106 + PS417_00405 0.41 -0.7 79,110 + PS417_00405 0.41 +0.5 79,304 + PS417_00405 0.48 -1.0 79,305 - PS417_00405 0.48 -0.2 79,305 - PS417_00405 0.48 -1.3 79,305 - PS417_00405 0.48 -1.7 79,308 + PS417_00405 0.48 -1.7 79,308 + PS417_00405 0.48 -0.4 79,309 - PS417_00405 0.48 -1.7 79,309 - PS417_00405 0.48 -0.5 79,358 + PS417_00405 0.50 -0.3 79,368 - PS417_00405 0.50 +1.7 79,514 - PS417_00405 0.55 +0.2 79,886 + PS417_00405 0.69 +0.0 80,070 + PS417_00405 0.75 -0.6 80,330 - PS417_00405 0.85 -0.7 80,339 + PS417_00405 0.85 -1.6 80,339 + PS417_00405 0.85 -0.5 80,369 + PS417_00405 0.86 -0.7 80,370 - PS417_00405 0.86 -1.8 80,370 - PS417_00405 0.86 -0.2 80,408 + PS417_00405 0.87 +0.1 80,799 - +0.3 80,829 + -0.7 80,853 + +0.4 80,854 - -0.6 80,911 + -0.3 80,911 + -0.2 80,911 + -0.7 80,911 + -0.6 80,912 - +0.1 80,912 - -0.3 80,912 - -0.0 80,921 + +1.2 80,921 + -0.2 80,921 + -0.4 80,936 - -0.1 81,740 + -0.1 81,740 + -0.9 81,740 + -0.3 81,740 + -0.7 81,742 + -0.5
Or see this region's nucleotide sequence