Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02200 and PS417_02205 are separated by 138 nucleotides PS417_02205 and PS417_02210 are separated by 72 nucleotides PS417_02210 and PS417_02215 are separated by 315 nucleotides
PS417_02200: PS417_02200 - lactate dehydrogenase, at 495,965 to 496,801
_02200
PS417_02205: PS417_02205 - peptide methionine sulfoxide reductase, at 496,940 to 497,587
_02205
PS417_02210: PS417_02210 - diguanylate cyclase, at 497,660 to 500,353
_02210
PS417_02215: PS417_02215 - dihydrolipoamide acetyltransferase, at 500,669 to 502,315
_02215
Position (kb) 497
498
499
500
501 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 496.810 kb on + strand at 496.811 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.863 kb on - strand at 496.870 kb on + strand at 496.873 kb on + strand at 496.874 kb on - strand at 496.941 kb on + strand at 496.981 kb on - strand at 497.005 kb on - strand, within PS417_02205 at 497.005 kb on - strand, within PS417_02205 at 497.016 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.031 kb on + strand, within PS417_02205 at 497.101 kb on - strand, within PS417_02205 at 497.151 kb on + strand, within PS417_02205 at 497.152 kb on - strand, within PS417_02205 at 497.166 kb on + strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.269 kb on - strand, within PS417_02205 at 497.322 kb on + strand, within PS417_02205 at 497.323 kb on - strand, within PS417_02205 at 497.822 kb on - strand at 497.941 kb on - strand, within PS417_02210 at 497.941 kb on - strand, within PS417_02210 at 499.038 kb on + strand, within PS417_02210 at 499.038 kb on + strand, within PS417_02210 at 499.252 kb on - strand, within PS417_02210 at 499.455 kb on - strand, within PS417_02210 at 499.983 kb on + strand, within PS417_02210 at 500.014 kb on + strand, within PS417_02210 at 500.015 kb on - strand, within PS417_02210 at 500.016 kb on + strand, within PS417_02210 at 500.016 kb on + strand, within PS417_02210 at 500.017 kb on - strand, within PS417_02210 at 500.161 kb on - strand at 500.257 kb on + strand at 500.258 kb on - strand at 500.472 kb on + strand at 500.472 kb on + strand at 500.472 kb on + strand at 500.473 kb on - strand at 500.474 kb on + strand at 500.475 kb on - strand at 500.475 kb on - strand at 500.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 496,810 + -0.2 496,811 - -0.7 496,863 - +0.2 496,863 - -0.1 496,863 - +0.9 496,863 - -0.7 496,870 + -0.0 496,873 + -0.1 496,874 - +0.5 496,941 + -0.5 496,981 - -0.1 497,005 - PS417_02205 0.10 +0.3 497,005 - PS417_02205 0.10 -0.1 497,016 + PS417_02205 0.12 +0.0 497,031 + PS417_02205 0.14 +0.4 497,031 + PS417_02205 0.14 -0.9 497,101 - PS417_02205 0.25 +0.2 497,151 + PS417_02205 0.33 +1.2 497,152 - PS417_02205 0.33 +0.7 497,166 + PS417_02205 0.35 +0.2 497,269 - PS417_02205 0.51 -1.1 497,269 - PS417_02205 0.51 +0.0 497,269 - PS417_02205 0.51 +0.1 497,269 - PS417_02205 0.51 -1.3 497,322 + PS417_02205 0.59 -0.1 497,323 - PS417_02205 0.59 +1.7 497,822 - -1.3 497,941 - PS417_02210 0.10 +0.1 497,941 - PS417_02210 0.10 +1.7 499,038 + PS417_02210 0.51 -1.7 499,038 + PS417_02210 0.51 -0.2 499,252 - PS417_02210 0.59 -0.7 499,455 - PS417_02210 0.67 -1.7 499,983 + PS417_02210 0.86 -0.3 500,014 + PS417_02210 0.87 -1.7 500,015 - PS417_02210 0.87 -3.0 500,016 + PS417_02210 0.87 +0.3 500,016 + PS417_02210 0.87 -0.4 500,017 - PS417_02210 0.87 -1.1 500,161 - +1.2 500,257 + -0.8 500,258 - -2.3 500,472 + -1.7 500,472 + +0.2 500,472 + +0.4 500,473 - +1.0 500,474 + +0.2 500,475 - -0.8 500,475 - +0.7 500,528 + +0.2
Or see this region's nucleotide sequence