Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18390 and PS417_18395 are separated by 3 nucleotides PS417_18395 and PS417_18400 overlap by 4 nucleotides
PS417_18390: PS417_18390 - LacI family transcriptional regulator, at 4,003,113 to 4,004,135
_18390
PS417_18395: PS417_18395 - D-ribose ABC transporter, permease component RbsC (from data) , at 4,004,139 to 4,005,116
_18395
PS417_18400: PS417_18400 - D-ribose ABC transporter, ATPase component RbsA (from data) , at 4,005,113 to 4,006,666
_18400
Position (kb)
4004
4005
4006 Strain fitness (log2 ratio)
-2
-1
0
1 at 4003.185 kb on - strand at 4003.298 kb on - strand, within PS417_18390 at 4003.298 kb on - strand, within PS417_18390 at 4003.460 kb on - strand, within PS417_18390 at 4003.719 kb on - strand, within PS417_18390 at 4003.774 kb on - strand, within PS417_18390 at 4003.774 kb on - strand, within PS417_18390 at 4003.817 kb on - strand, within PS417_18390 at 4003.817 kb on - strand, within PS417_18390 at 4003.875 kb on - strand at 4003.886 kb on - strand, within PS417_18390 at 4004.003 kb on - strand, within PS417_18390 at 4004.003 kb on - strand, within PS417_18390 at 4004.003 kb on - strand, within PS417_18390 at 4004.092 kb on + strand at 4004.281 kb on - strand, within PS417_18395 at 4004.545 kb on - strand, within PS417_18395 at 4004.660 kb on - strand, within PS417_18395 at 4004.941 kb on - strand, within PS417_18395 at 4005.810 kb on + strand, within PS417_18400 at 4005.810 kb on + strand, within PS417_18400 at 4005.810 kb on + strand, within PS417_18400 at 4005.972 kb on + strand, within PS417_18400 at 4005.973 kb on - strand, within PS417_18400
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,003,185 - +1.0 4,003,298 - PS417_18390 0.18 -0.1 4,003,298 - PS417_18390 0.18 +0.2 4,003,460 - PS417_18390 0.34 -0.1 4,003,719 - PS417_18390 0.59 +0.7 4,003,774 - PS417_18390 0.65 -0.0 4,003,774 - PS417_18390 0.65 +0.1 4,003,817 - PS417_18390 0.69 +0.0 4,003,817 - PS417_18390 0.69 +0.9 4,003,875 - -1.3 4,003,886 - PS417_18390 0.76 -0.3 4,004,003 - PS417_18390 0.87 +0.2 4,004,003 - PS417_18390 0.87 -0.0 4,004,003 - PS417_18390 0.87 -0.4 4,004,092 + -0.9 4,004,281 - PS417_18395 0.15 +0.7 4,004,545 - PS417_18395 0.42 -1.1 4,004,660 - PS417_18395 0.53 -1.9 4,004,941 - PS417_18395 0.82 -0.3 4,005,810 + PS417_18400 0.45 -0.7 4,005,810 + PS417_18400 0.45 +0.7 4,005,810 + PS417_18400 0.45 +0.2 4,005,972 + PS417_18400 0.55 -0.7 4,005,973 - PS417_18400 0.55 -0.7
Or see this region's nucleotide sequence