Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18035 and PS417_18040 are separated by 5 nucleotides PS417_18040 and PS417_18045 overlap by 4 nucleotides PS417_18045 and PS417_18050 overlap by 26 nucleotides
PS417_18035: PS417_18035 - general secretion pathway protein GspD, at 3,930,311 to 3,932,422
_18035
PS417_18040: PS417_18040 - general secretion pathway protein GspN, at 3,932,428 to 3,932,946
_18040
PS417_18045: PS417_18045 - general secretion pathway protein GspM, at 3,932,943 to 3,933,545
_18045
PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512
_18050
Position (kb)
3932
3933 Strain fitness (log2 ratio)
-1
0
1
2 at 3931.443 kb on + strand, within PS417_18035 at 3931.443 kb on + strand at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.444 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.720 kb on - strand, within PS417_18035 at 3931.843 kb on - strand, within PS417_18035 at 3931.947 kb on + strand, within PS417_18035 at 3932.083 kb on - strand, within PS417_18035 at 3932.454 kb on + strand at 3932.894 kb on - strand, within PS417_18040 at 3933.382 kb on + strand, within PS417_18045 at 3933.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 3,931,443 + PS417_18035 0.54 +0.3 3,931,443 + +2.3 3,931,444 - PS417_18035 0.54 -0.4 3,931,444 - PS417_18035 0.54 -0.6 3,931,444 - PS417_18035 0.54 +0.7 3,931,720 - PS417_18035 0.67 -0.1 3,931,720 - PS417_18035 0.67 -0.1 3,931,843 - PS417_18035 0.73 -0.9 3,931,947 + PS417_18035 0.77 +2.3 3,932,083 - PS417_18035 0.84 +0.5 3,932,454 + -0.6 3,932,894 - PS417_18040 0.90 -1.1 3,933,382 + PS417_18045 0.73 -0.6 3,933,562 + -0.6
Or see this region's nucleotide sequence