Experiment: KB with d-limonene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1822 and Psyr_1823 are separated by 5 nucleotides Psyr_1823 and Psyr_1824 are separated by 93 nucleotides Psyr_1824 and Psyr_1825 are separated by 100 nucleotides
Psyr_1822: Psyr_1822 - MscS Mechanosensitive ion channel, at 2,102,041 to 2,103,165
_1822
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Psyr_1824: Psyr_1824 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,104,950 to 2,105,744
_1824
Psyr_1825: Psyr_1825 - DNA polymerase III, tau subunit, at 2,105,845 to 2,108,055
_1825
Position (kb)
2103
2104
2105 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2102.218 kb on + strand, within Psyr_1822 at 2102.218 kb on + strand, within Psyr_1822 at 2102.218 kb on + strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.251 kb on - strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.408 kb on - strand, within Psyr_1822 at 2102.791 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.995 kb on + strand, within Psyr_1822 at 2102.995 kb on + strand, within Psyr_1822 at 2102.995 kb on + strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.006 kb on + strand, within Psyr_1822 at 2103.006 kb on + strand, within Psyr_1822 at 2103.094 kb on + strand at 2103.094 kb on + strand at 2103.095 kb on - strand at 2103.118 kb on - strand at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.578 kb on - strand, within Psyr_1823 at 2103.578 kb on - strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.713 kb on - strand, within Psyr_1823 at 2103.967 kb on + strand, within Psyr_1823 at 2103.967 kb on + strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2104.258 kb on + strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.259 kb on - strand, within Psyr_1823 at 2104.343 kb on - strand, within Psyr_1823 at 2104.558 kb on + strand, within Psyr_1823 at 2104.558 kb on + strand, within Psyr_1823 at 2104.643 kb on - strand, within Psyr_1823 at 2104.753 kb on + strand at 2104.754 kb on - strand at 2104.754 kb on - strand at 2104.760 kb on + strand at 2104.907 kb on + strand at 2104.919 kb on - strand at 2104.919 kb on - strand at 2104.972 kb on + strand at 2104.973 kb on - strand at 2105.173 kb on + strand, within Psyr_1824 at 2105.173 kb on + strand, within Psyr_1824 at 2105.173 kb on + strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.374 kb on + strand, within Psyr_1824 at 2105.375 kb on - strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with d-limonene 10 mM remove 2,102,218 + Psyr_1822 0.16 -0.1 2,102,218 + Psyr_1822 0.16 -2.0 2,102,218 + Psyr_1822 0.16 -0.1 2,102,219 - Psyr_1822 0.16 +0.2 2,102,219 - Psyr_1822 0.16 +0.8 2,102,250 + Psyr_1822 0.19 -0.8 2,102,250 + Psyr_1822 0.19 -0.9 2,102,250 + Psyr_1822 0.19 +1.6 2,102,251 - Psyr_1822 0.19 -0.9 2,102,253 + Psyr_1822 0.19 -2.1 2,102,253 + Psyr_1822 0.19 +1.2 2,102,253 + Psyr_1822 0.19 -0.1 2,102,253 + Psyr_1822 0.19 +0.6 2,102,253 + Psyr_1822 0.19 +0.3 2,102,253 + Psyr_1822 0.19 +1.6 2,102,253 + Psyr_1822 0.19 -2.7 2,102,254 - Psyr_1822 0.19 +0.4 2,102,254 - Psyr_1822 0.19 -1.3 2,102,408 - Psyr_1822 0.33 -0.3 2,102,791 + Psyr_1822 0.67 +0.4 2,102,839 + Psyr_1822 0.71 -0.1 2,102,839 + Psyr_1822 0.71 +0.4 2,102,839 + Psyr_1822 0.71 -0.1 2,102,839 + Psyr_1822 0.71 +3.0 2,102,839 + Psyr_1822 0.71 -0.5 2,102,840 - Psyr_1822 0.71 -0.3 2,102,840 - Psyr_1822 0.71 -1.6 2,102,840 - Psyr_1822 0.71 -2.1 2,102,840 - Psyr_1822 0.71 -0.8 2,102,840 - Psyr_1822 0.71 +0.2 2,102,995 + Psyr_1822 0.85 +0.1 2,102,995 + Psyr_1822 0.85 -2.1 2,102,995 + Psyr_1822 0.85 +1.3 2,102,996 - Psyr_1822 0.85 -1.4 2,102,996 - Psyr_1822 0.85 -1.4 2,103,004 + Psyr_1822 0.86 -2.4 2,103,004 + Psyr_1822 0.86 +1.6 2,103,004 + Psyr_1822 0.86 +0.7 2,103,004 + Psyr_1822 0.86 +0.4 2,103,005 - Psyr_1822 0.86 -0.9 2,103,005 - Psyr_1822 0.86 +0.3 2,103,005 - Psyr_1822 0.86 -0.3 2,103,005 - Psyr_1822 0.86 +1.8 2,103,005 - Psyr_1822 0.86 -1.0 2,103,006 + Psyr_1822 0.86 +0.4 2,103,006 + Psyr_1822 0.86 +0.1 2,103,094 + +0.1 2,103,094 + -0.4 2,103,095 - +1.5 2,103,118 - -0.7 2,103,577 + Psyr_1823 0.24 -0.8 2,103,577 + Psyr_1823 0.24 -0.2 2,103,578 - Psyr_1823 0.24 -0.5 2,103,578 - Psyr_1823 0.24 +0.4 2,103,712 + Psyr_1823 0.32 +0.7 2,103,712 + Psyr_1823 0.32 +1.2 2,103,713 - Psyr_1823 0.32 +0.8 2,103,967 + Psyr_1823 0.47 +0.4 2,103,967 + Psyr_1823 0.47 +0.7 2,103,968 - Psyr_1823 0.47 -1.4 2,103,968 - Psyr_1823 0.47 -0.0 2,104,258 + Psyr_1823 0.64 -0.5 2,104,259 - Psyr_1823 0.65 +0.9 2,104,259 - Psyr_1823 0.65 -2.3 2,104,259 - Psyr_1823 0.65 -1.6 2,104,343 - Psyr_1823 0.70 +0.0 2,104,558 + Psyr_1823 0.82 -2.4 2,104,558 + Psyr_1823 0.82 -0.1 2,104,643 - Psyr_1823 0.87 -0.1 2,104,753 + +0.6 2,104,754 - -0.9 2,104,754 - -0.9 2,104,760 + -1.7 2,104,907 + +0.0 2,104,919 - +0.4 2,104,919 - -0.9 2,104,972 + +0.3 2,104,973 - +0.6 2,105,173 + Psyr_1824 0.28 -0.9 2,105,173 + Psyr_1824 0.28 -0.9 2,105,173 + Psyr_1824 0.28 -1.5 2,105,174 - Psyr_1824 0.28 -0.5 2,105,174 - Psyr_1824 0.28 -2.4 2,105,174 - Psyr_1824 0.28 -0.9 2,105,374 + Psyr_1824 0.53 +1.0 2,105,375 - Psyr_1824 0.53 -1.0 2,105,437 + Psyr_1824 0.61 -0.8 2,105,437 + Psyr_1824 0.61 +2.6 2,105,437 + Psyr_1824 0.61 +0.4 2,105,438 - Psyr_1824 0.61 -0.9 2,105,671 - -0.1 2,105,671 - +0.6 2,105,671 - +0.2 2,105,671 - +0.1 2,105,765 - -1.4
Or see this region's nucleotide sequence