Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2718

Experiment: KB with Lupeol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2716 and Psyr_2717 are separated by 200 nucleotidesPsyr_2717 and Psyr_2718 are separated by 26 nucleotidesPsyr_2718 and Psyr_2719 are separated by 321 nucleotidesPsyr_2719 and Psyr_2720 are separated by 5 nucleotides Psyr_2716: Psyr_2716 - Mandelate racemase/muconate lactonizing enzyme:Mandelate racemase/muconate lactonizing enzyme, at 3,298,441 to 3,299,715 _2716 Psyr_2717: Psyr_2717 - Antibiotic biosynthesis monooxygenase, at 3,299,916 to 3,300,224 _2717 Psyr_2718: Psyr_2718 - Major royal jelly protein, at 3,300,251 to 3,301,435 _2718 Psyr_2719: Psyr_2719 - transcriptional regulator, TetR family, at 3,301,757 to 3,302,338 _2719 Psyr_2720: Psyr_2720 - Urea amidolyase-related protein, at 3,302,344 to 3,303,318 _2720 Position (kb) 3300 3301 3302Strain fitness (log2 ratio) -2 -1 0 1 2at 3299.596 kb on + strandat 3299.597 kb on - strandat 3299.677 kb on + strandat 3299.677 kb on + strandat 3299.678 kb on - strandat 3299.678 kb on - strandat 3299.678 kb on - strandat 3299.678 kb on - strandat 3299.710 kb on + strandat 3299.710 kb on + strandat 3299.711 kb on - strandat 3299.767 kb on - strandat 3299.940 kb on + strandat 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.055 kb on + strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.056 kb on - strand, within Psyr_2717at 3300.095 kb on - strand, within Psyr_2717at 3300.095 kb on - strand, within Psyr_2717at 3300.369 kb on + strandat 3300.369 kb on + strandat 3300.369 kb on + strandat 3300.370 kb on - strand, within Psyr_2718at 3300.370 kb on - strand, within Psyr_2718at 3300.376 kb on - strand, within Psyr_2718at 3300.475 kb on + strand, within Psyr_2718at 3300.475 kb on + strand, within Psyr_2718at 3300.476 kb on - strand, within Psyr_2718at 3300.476 kb on - strand, within Psyr_2718at 3300.497 kb on + strand, within Psyr_2718at 3300.621 kb on + strand, within Psyr_2718at 3300.621 kb on + strand, within Psyr_2718at 3300.622 kb on - strand, within Psyr_2718at 3300.651 kb on + strand, within Psyr_2718at 3300.651 kb on + strand, within Psyr_2718at 3300.785 kb on - strand, within Psyr_2718at 3300.785 kb on - strand, within Psyr_2718at 3300.876 kb on - strand, within Psyr_2718at 3300.876 kb on - strand, within Psyr_2718at 3300.876 kb on - strand, within Psyr_2718at 3300.876 kb on - strand, within Psyr_2718at 3300.879 kb on + strand, within Psyr_2718at 3300.879 kb on + strand, within Psyr_2718at 3300.879 kb on + strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.880 kb on - strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.930 kb on + strand, within Psyr_2718at 3300.931 kb on - strand, within Psyr_2718at 3300.931 kb on - strand, within Psyr_2718at 3300.931 kb on - strand, within Psyr_2718at 3300.931 kb on - strand, within Psyr_2718at 3301.198 kb on + strand, within Psyr_2718at 3301.284 kb on + strand, within Psyr_2718at 3301.378 kb on + strandat 3301.590 kb on - strandat 3301.625 kb on - strandat 3301.625 kb on - strandat 3301.699 kb on + strandat 3301.699 kb on + strandat 3301.766 kb on - strandat 3301.766 kb on - strandat 3301.766 kb on - strandat 3301.766 kb on - strandat 3301.839 kb on - strand, within Psyr_2719at 3301.898 kb on + strand, within Psyr_2719at 3302.007 kb on - strand, within Psyr_2719at 3302.388 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM
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3,299,596 + -1.4
3,299,597 - +2.4
3,299,677 + +0.8
3,299,677 + +0.7
3,299,678 - +1.2
3,299,678 - -1.6
3,299,678 - -0.7
3,299,678 - -1.0
3,299,710 + -0.4
3,299,710 + +0.2
3,299,711 - -0.8
3,299,767 - -1.0
3,299,940 + +1.3
3,300,055 + Psyr_2717 0.45 +0.3
3,300,055 + Psyr_2717 0.45 +0.9
3,300,055 + Psyr_2717 0.45 +1.0
3,300,055 + Psyr_2717 0.45 +0.6
3,300,055 + Psyr_2717 0.45 -0.2
3,300,055 + Psyr_2717 0.45 +1.3
3,300,055 + Psyr_2717 0.45 +0.3
3,300,056 - Psyr_2717 0.45 +0.8
3,300,056 - Psyr_2717 0.45 -0.7
3,300,056 - Psyr_2717 0.45 +0.7
3,300,056 - Psyr_2717 0.45 +1.0
3,300,056 - Psyr_2717 0.45 -0.6
3,300,056 - Psyr_2717 0.45 -0.9
3,300,095 - Psyr_2717 0.58 -0.5
3,300,095 - Psyr_2717 0.58 +2.2
3,300,369 + +2.1
3,300,369 + -2.3
3,300,369 + -0.9
3,300,370 - Psyr_2718 0.10 +0.6
3,300,370 - Psyr_2718 0.10 -0.6
3,300,376 - Psyr_2718 0.11 -0.6
3,300,475 + Psyr_2718 0.19 +1.8
3,300,475 + Psyr_2718 0.19 -1.7
3,300,476 - Psyr_2718 0.19 -0.5
3,300,476 - Psyr_2718 0.19 -0.6
3,300,497 + Psyr_2718 0.21 +0.1
3,300,621 + Psyr_2718 0.31 -0.2
3,300,621 + Psyr_2718 0.31 -0.2
3,300,622 - Psyr_2718 0.31 +0.3
3,300,651 + Psyr_2718 0.34 +0.4
3,300,651 + Psyr_2718 0.34 -1.5
3,300,785 - Psyr_2718 0.45 -0.7
3,300,785 - Psyr_2718 0.45 +1.7
3,300,876 - Psyr_2718 0.53 +1.4
3,300,876 - Psyr_2718 0.53 -1.0
3,300,876 - Psyr_2718 0.53 +0.4
3,300,876 - Psyr_2718 0.53 +0.6
3,300,879 + Psyr_2718 0.53 +2.4
3,300,879 + Psyr_2718 0.53 -0.2
3,300,879 + Psyr_2718 0.53 -0.6
3,300,880 - Psyr_2718 0.53 -0.2
3,300,880 - Psyr_2718 0.53 +0.9
3,300,880 - Psyr_2718 0.53 -0.4
3,300,880 - Psyr_2718 0.53 +0.2
3,300,880 - Psyr_2718 0.53 +0.3
3,300,880 - Psyr_2718 0.53 -1.0
3,300,930 + Psyr_2718 0.57 -0.4
3,300,930 + Psyr_2718 0.57 +1.2
3,300,930 + Psyr_2718 0.57 -1.4
3,300,930 + Psyr_2718 0.57 +0.2
3,300,930 + Psyr_2718 0.57 +0.2
3,300,930 + Psyr_2718 0.57 +2.4
3,300,931 - Psyr_2718 0.57 +0.1
3,300,931 - Psyr_2718 0.57 +1.4
3,300,931 - Psyr_2718 0.57 +0.0
3,300,931 - Psyr_2718 0.57 -1.2
3,301,198 + Psyr_2718 0.80 +0.6
3,301,284 + Psyr_2718 0.87 +0.5
3,301,378 + +0.0
3,301,590 - -0.4
3,301,625 - -0.7
3,301,625 - -0.9
3,301,699 + +0.4
3,301,699 + -0.3
3,301,766 - -0.1
3,301,766 - +0.8
3,301,766 - +0.8
3,301,766 - +1.8
3,301,839 - Psyr_2719 0.14 -0.7
3,301,898 + Psyr_2719 0.24 -0.9
3,302,007 - Psyr_2719 0.43 -0.8
3,302,388 - +1.3

Or see this region's nucleotide sequence